Strain Fitness in Escherichia coli BW25113 around b1610

Experiment: LB Anaerobic with Trimethoprim 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrstB and tus are separated by 75 nucleotidestus and fumC overlap by 4 nucleotides b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207 rstB b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212 tus b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612 fumC Position (kb) 1682 1683 1684Strain fitness (log2 ratio) -2 -1 0 1 2at 1681.555 kb on + strand, within rstBat 1681.555 kb on + strand, within rstBat 1681.779 kb on - strand, within rstBat 1681.779 kb on - strand, within rstBat 1681.827 kb on + strand, within rstBat 1682.011 kb on + strand, within rstBat 1682.068 kb on + strand, within rstBat 1682.068 kb on + strand, within rstBat 1682.090 kb on + strandat 1682.098 kb on - strandat 1682.100 kb on - strandat 1682.102 kb on + strandat 1682.102 kb on + strandat 1682.223 kb on + strandat 1682.223 kb on + strandat 1682.306 kb on - strandat 1682.451 kb on - strand, within tusat 1682.567 kb on - strand, within tusat 1682.620 kb on + strand, within tusat 1682.620 kb on + strand, within tusat 1682.820 kb on + strand, within tusat 1682.838 kb on + strand, within tusat 1682.878 kb on + strand, within tusat 1682.886 kb on - strand, within tusat 1682.941 kb on - strand, within tusat 1682.941 kb on - strand, within tusat 1683.046 kb on + strand, within tusat 1683.046 kb on + strand, within tusat 1683.207 kb on + strandat 1683.241 kb on + strandat 1683.295 kb on + strandat 1683.295 kb on + strandat 1683.439 kb on + strand, within fumCat 1683.595 kb on + strand, within fumCat 1683.612 kb on + strand, within fumCat 1683.612 kb on + strand, within fumCat 1683.665 kb on - strand, within fumCat 1683.665 kb on - strand, within fumCat 1683.709 kb on + strand, within fumCat 1683.709 kb on + strand, within fumCat 1683.859 kb on - strand, within fumCat 1683.859 kb on - strand, within fumCat 1683.981 kb on - strand, within fumCat 1684.016 kb on - strand, within fumCat 1684.016 kb on - strand, within fumC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Trimethoprim 0.0000003125 mM
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1,681,555 + rstB b1609 0.50 -2.0
1,681,555 + rstB b1609 0.50 -0.5
1,681,779 - rstB b1609 0.67 +1.3
1,681,779 - rstB b1609 0.67 +0.3
1,681,827 + rstB b1609 0.71 -1.3
1,682,011 + rstB b1609 0.85 -0.5
1,682,068 + rstB b1609 0.89 -2.5
1,682,068 + rstB b1609 0.89 -0.8
1,682,090 + +0.3
1,682,098 - -1.8
1,682,100 - +0.9
1,682,102 + -1.3
1,682,102 + +0.7
1,682,223 + -1.0
1,682,223 + +0.5
1,682,306 - +0.1
1,682,451 - tus b1610 0.18 +0.0
1,682,567 - tus b1610 0.31 +0.5
1,682,620 + tus b1610 0.36 +0.9
1,682,620 + tus b1610 0.36 +0.5
1,682,820 + tus b1610 0.58 -0.1
1,682,838 + tus b1610 0.60 -0.1
1,682,878 + tus b1610 0.64 +0.2
1,682,886 - tus b1610 0.65 +0.5
1,682,941 - tus b1610 0.71 +0.1
1,682,941 - tus b1610 0.71 -0.5
1,683,046 + tus b1610 0.82 +0.9
1,683,046 + tus b1610 0.82 -0.6
1,683,207 + +0.4
1,683,241 + +0.5
1,683,295 + -1.5
1,683,295 + -0.2
1,683,439 + fumC b1611 0.16 +0.9
1,683,595 + fumC b1611 0.27 +0.0
1,683,612 + fumC b1611 0.29 +0.1
1,683,612 + fumC b1611 0.29 -0.8
1,683,665 - fumC b1611 0.32 +1.5
1,683,665 - fumC b1611 0.32 -0.4
1,683,709 + fumC b1611 0.36 -1.5
1,683,709 + fumC b1611 0.36 +2.1
1,683,859 - fumC b1611 0.46 -0.1
1,683,859 - fumC b1611 0.46 +0.5
1,683,981 - fumC b1611 0.55 +0.0
1,684,016 - fumC b1611 0.57 +0.5
1,684,016 - fumC b1611 0.57 +0.2

Or see this region's nucleotide sequence