Experiment: Growth on radish seeds for 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06330 and PS417_06335 are separated by 8 nucleotides PS417_06335 and PS417_06340 are separated by 54 nucleotides PS417_06340 and PS417_06345 overlap by 4 nucleotides
PS417_06330: PS417_06330 - S-(hydroxymethyl)glutathione dehydrogenase, at 1,406,099 to 1,407,211
_06330
PS417_06335: PS417_06335 - S-formylglutathione hydrolase, at 1,407,220 to 1,408,065
_06335
PS417_06340: PS417_06340 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,408,120 to 1,408,593
_06340
PS417_06345: PS417_06345 - pseudouridine synthase, at 1,408,590 to 1,409,648
_06345
Position (kb) 1408
1409 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1407.274 kb on + strand at 1407.541 kb on + strand, within PS417_06335 at 1407.592 kb on + strand, within PS417_06335 at 1407.592 kb on + strand at 1407.593 kb on - strand, within PS417_06335 at 1407.593 kb on - strand, within PS417_06335 at 1407.593 kb on - strand, within PS417_06335 at 1407.989 kb on - strand at 1408.000 kb on + strand at 1408.001 kb on - strand at 1408.001 kb on - strand at 1408.815 kb on + strand, within PS417_06345 at 1408.815 kb on + strand, within PS417_06345 at 1408.816 kb on - strand, within PS417_06345 at 1408.816 kb on - strand, within PS417_06345 at 1409.070 kb on + strand, within PS417_06345 at 1409.070 kb on + strand, within PS417_06345 at 1409.070 kb on + strand, within PS417_06345 at 1409.071 kb on - strand, within PS417_06345
Per-strain Table
Position Strand Gene LocusTag Fraction Growth on radish seeds for 72 hours remove 1,407,274 + +0.7 1,407,541 + PS417_06335 0.38 -1.0 1,407,592 + PS417_06335 0.44 +0.0 1,407,592 + +1.0 1,407,593 - PS417_06335 0.44 -0.6 1,407,593 - PS417_06335 0.44 +0.8 1,407,593 - PS417_06335 0.44 -1.8 1,407,989 - -1.7 1,408,000 + +0.3 1,408,001 - +0.0 1,408,001 - -1.2 1,408,815 + PS417_06345 0.21 -0.2 1,408,815 + PS417_06345 0.21 +0.5 1,408,816 - PS417_06345 0.21 -0.5 1,408,816 - PS417_06345 0.21 +1.0 1,409,070 + PS417_06345 0.45 +0.3 1,409,070 + PS417_06345 0.45 +1.0 1,409,070 + PS417_06345 0.45 +2.0 1,409,071 - PS417_06345 0.45 +0.7
Or see this region's nucleotide sequence