Strain Fitness in Escherichia coli BW25113 around b0985

Experiment: LB Anaerobic with Spectinomycin 0.00003125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Spectinomycin 0.00003125 mM
1,046,171	+	ymcA	b0984	0.52	+0.3
1,046,193	+	ymcA	b0984	0.53	+0.1
1,046,193	+	ymcA	b0984	0.53	+0.8
1,046,372	+	ymcA	b0984	0.62	+1.0
1,046,390	-	ymcA	b0984	0.63	-0.2
1,046,537	-	ymcA	b0984	0.70	-0.6
1,046,537	-	ymcA	b0984	0.70	-1.0
1,046,618	-	ymcA	b0984	0.74	+0.2
1,046,663	-	ymcA	b0984	0.76	+0.3
1,046,663	-	ymcA	b0984	0.76	+0.6
1,046,683	+	ymcA	b0984	0.77	-0.0
1,046,688	+	ymcA	b0984	0.77	+0.7
1,046,697	-	ymcA	b0984	0.77	-0.4
1,046,852	+	ymcA	b0984	0.85	-0.1
1,046,852	+	ymcA	b0984	0.85	+0.2
1,046,852	+	ymcA	b0984	0.85	+0.4
1,046,867	-	ymcA	b0984	0.86	+0.0
1,046,898	-	ymcA	b0984	0.87	+0.3
1,046,910	-	ymcA	b0984	0.88	-1.6
1,046,910	-	ymcA	b0984	0.88	+0.2
1,046,941	-	ymcA	b0984	0.89	+1.1
1,046,957	-	ymcA	b0984	0.90	+0.3
1,046,957	-	ymcA	b0984	0.90	+0.1
1,046,960	+				-0.4
1,046,960	+				+0.6
1,046,971	+				+1.1
1,046,971	+				-0.7
1,046,973	-				-0.3
1,047,102	-				+0.3
1,047,116	+				-0.0
1,047,482	+	ymcB	b0985	0.42	+1.1
1,047,487	+	ymcB	b0985	0.43	-0.0
1,047,487	+	ymcB	b0985	0.43	+0.9
1,047,487	+	ymcB	b0985	0.43	+0.9
1,047,487	+	ymcB	b0985	0.43	-0.8
1,047,493	+	ymcB	b0985	0.44	-0.8
1,047,495	-	ymcB	b0985	0.44	+0.7
1,047,495	-	ymcB	b0985	0.44	+0.3
1,047,533	-	ymcB	b0985	0.49	+1.2
1,047,533	-	ymcB	b0985	0.49	+0.7
1,047,638	-	ymcB	b0985	0.63	+0.6
1,047,641	-	ymcB	b0985	0.63	-1.9
1,047,827	+	ymcB	b0985	0.88	+0.4
1,047,848	+				+1.0
1,047,856	-				+0.8
1,047,861	+				-0.2
1,047,880	-				+0.3
1,047,952	+				-0.3
1,047,955	-				-1.6
1,047,989	+	ymcC	b0986	0.12	+0.1
1,047,998	-	ymcC	b0986	0.13	+0.4
1,048,018	-	ymcC	b0986	0.17	+0.8
1,048,050	+	ymcC	b0986	0.22	-0.2
1,048,050	+	ymcC	b0986	0.22	+1.6
1,048,051	+	ymcC	b0986	0.22	-1.8
1,048,051	+	ymcC	b0986	0.22	-0.2
1,048,058	-	ymcC	b0986	0.23	+0.7
1,048,060	+	ymcC	b0986	0.23	+0.4
1,048,065	+	ymcC	b0986	0.24	+0.5
1,048,130	+	ymcC	b0986	0.34	+0.2
1,048,130	+	ymcC	b0986	0.34	-0.6
1,048,172	-	ymcC	b0986	0.40	+0.6
1,048,189	-	ymcC	b0986	0.43	+0.6
1,048,189	-	ymcC	b0986	0.43	-1.0
1,048,198	+	ymcC	b0986	0.44	+0.9
1,048,201	-	ymcC	b0986	0.45	-2.0
1,048,206	-	ymcC	b0986	0.46	-0.4
1,048,206	-	ymcC	b0986	0.46	-0.8
1,048,212	+	ymcC	b0986	0.47	-1.2
1,048,212	+	ymcC	b0986	0.47	+0.6
1,048,217	+	ymcC	b0986	0.47	-0.0
1,048,220	+	ymcC	b0986	0.48	-0.4
1,048,221	+	ymcC	b0986	0.48	-0.3
1,048,228	-	ymcC	b0986	0.49	+0.2
1,048,277	+	ymcC	b0986	0.57	-1.0
1,048,291	-	ymcC	b0986	0.59	+0.1
1,048,297	+	ymcC	b0986	0.60	+0.8
1,048,297	+	ymcC	b0986	0.60	+0.2
1,048,297	+	ymcC	b0986	0.60	+1.6
1,048,300	-	ymcC	b0986	0.60	+0.6
1,048,304	-	ymcC	b0986	0.61	-0.4
1,048,306	+	ymcC	b0986	0.61	-1.0
1,048,306	+	ymcC	b0986	0.61	-0.4
1,048,310	+	ymcC	b0986	0.62	+0.7
1,048,311	+	ymcC	b0986	0.62	-0.7
1,048,311	+	ymcC	b0986	0.62	-0.4
1,048,311	+	ymcC	b0986	0.62	-0.1
1,048,311	+	ymcC	b0986	0.62	+0.6
1,048,311	+	ymcC	b0986	0.62	+1.1
1,048,313	-	ymcC	b0986	0.62	-0.4
1,048,324	-	ymcC	b0986	0.64	+0.6
1,048,329	-	ymcC	b0986	0.65	-0.0
1,048,350	+	ymcC	b0986	0.68	-0.6
1,048,355	+	ymcC	b0986	0.69	-0.5
1,048,355	+	ymcC	b0986	0.69	+1.0
1,048,355	+	ymcC	b0986	0.69	+0.8
1,048,363	-	ymcC	b0986	0.70	+0.6
1,048,363	-	ymcC	b0986	0.70	+0.6
1,048,363	-	ymcC	b0986	0.70	-0.4
1,048,363	-	ymcC	b0986	0.70	+0.6
1,048,402	+	ymcC	b0986	0.76	-1.0
1,048,413	+	ymcC	b0986	0.78	-1.0
1,048,413	+	ymcC	b0986	0.78	+1.1
1,048,417	+	ymcC	b0986	0.78	-1.8
1,048,426	-	ymcC	b0986	0.80	+1.7
1,048,432	-	ymcC	b0986	0.81	-1.9
1,048,437	+	ymcC	b0986	0.82	-0.3
1,048,443	-	ymcC	b0986	0.82	-0.4
1,048,443	-	ymcC	b0986	0.82	-0.4
1,048,451	-	ymcC	b0986	0.84	-0.4
1,048,451	-	ymcC	b0986	0.84	+1.6
1,048,502	+				-1.4
1,048,503	+				-1.6
1,048,503	+				+0.3
1,048,503	+				-0.4
1,048,504	+				+0.3
1,048,511	-				+2.9
1,048,511	-				-0.8
1,048,511	-				-0.4
1,048,511	-				-1.0
1,048,513	+				+0.2
1,048,523	-				-1.0
1,048,523	-				-0.4
1,048,555	+				+0.7
1,048,611	-				+0.4
1,048,657	-				+1.3
1,048,667	-				-1.8
1,048,667	-				+0.1
1,048,667	-				+0.6
1,048,667	-				-1.2
1,048,667	-				-0.4
1,048,677	-				+0.0
1,048,681	-				-0.4
1,048,681	-				+0.3
1,048,684	+				+0.6
1,048,689	+				+0.6
1,048,691	-				+0.4
1,048,691	-				-2.8
1,048,692	+				+0.6
1,048,697	-	ymcD	b0987	0.11	-1.8
1,048,698	-	ymcD	b0987	0.12	-0.4
1,048,698	-	ymcD	b0987	0.12	+0.1
1,048,700	-	ymcD	b0987	0.12	+1.0
1,048,700	-	ymcD	b0987	0.12	+0.9
1,048,700	-	ymcD	b0987	0.12	+0.2
1,048,700	-	ymcD	b0987	0.12	+0.6
1,048,700	-	ymcD	b0987	0.12	-0.3
1,048,704	+	ymcD	b0987	0.14	+1.6
1,048,711	-	ymcD	b0987	0.16	+1.6
1,048,716	+	ymcD	b0987	0.18	-1.4
1,048,793	+	ymcD	b0987	0.43	-0.7
1,048,807	+	ymcD	b0987	0.47	+0.1
1,048,813	+	ymcD	b0987	0.49	-0.3
1,048,892	-	ymcD	b0987	0.75	+0.4