Experiment: KB with Cotarnine Chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4302 and Psyr_4303 are separated by 197 nucleotides Psyr_4303 and Psyr_4304 are separated by 33 nucleotides Psyr_4304 and Psyr_4305 are separated by 184 nucleotides Psyr_4305 and Psyr_4306 are separated by 104 nucleotides
Psyr_4302: Psyr_4302 - conserved hypothetical protein, at 5,128,171 to 5,128,875
_4302
Psyr_4303: Psyr_4303 - Protein of unknown function UPF0259, at 5,129,073 to 5,129,735
_4303
Psyr_4304: Psyr_4304 - Endonuclease/exonuclease/phosphatase, at 5,129,769 to 5,130,911
_4304
Psyr_4305: Psyr_4305 - conserved hypothetical protein, at 5,131,096 to 5,131,344
_4305
Psyr_4306: Psyr_4306 - conserved hypothetical protein, at 5,131,449 to 5,131,646
_4306
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5129.035 kb on + strand at 5129.055 kb on - strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.106 kb on + strand at 5129.107 kb on - strand at 5129.120 kb on + strand at 5129.126 kb on - strand at 5129.126 kb on - strand at 5129.223 kb on + strand, within Psyr_4303 at 5129.224 kb on - strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.259 kb on + strand, within Psyr_4303 at 5129.259 kb on + strand, within Psyr_4303 at 5129.260 kb on - strand, within Psyr_4303 at 5129.260 kb on - strand, within Psyr_4303 at 5129.260 kb on - strand, within Psyr_4303 at 5129.261 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.327 kb on - strand, within Psyr_4303 at 5129.327 kb on - strand, within Psyr_4303 at 5129.327 kb on - strand, within Psyr_4303 at 5129.327 kb on - strand, within Psyr_4303 at 5129.327 kb on - strand, within Psyr_4303 at 5129.478 kb on - strand, within Psyr_4303 at 5129.554 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.830 kb on + strand at 5129.837 kb on - strand at 5129.837 kb on - strand at 5129.837 kb on - strand at 5129.850 kb on + strand at 5129.873 kb on - strand at 5129.923 kb on + strand, within Psyr_4304 at 5129.923 kb on + strand, within Psyr_4304 at 5129.923 kb on + strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5130.009 kb on + strand, within Psyr_4304 at 5130.027 kb on + strand, within Psyr_4304 at 5130.040 kb on - strand, within Psyr_4304 at 5130.097 kb on - strand, within Psyr_4304 at 5130.189 kb on + strand, within Psyr_4304 at 5130.190 kb on - strand, within Psyr_4304 at 5130.259 kb on - strand, within Psyr_4304 at 5130.291 kb on - strand, within Psyr_4304 at 5130.291 kb on - strand, within Psyr_4304 at 5130.429 kb on + strand, within Psyr_4304 at 5130.429 kb on + strand, within Psyr_4304 at 5130.430 kb on - strand, within Psyr_4304 at 5130.645 kb on + strand, within Psyr_4304 at 5130.645 kb on + strand, within Psyr_4304 at 5130.673 kb on + strand, within Psyr_4304 at 5130.674 kb on - strand, within Psyr_4304 at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.939 kb on - strand at 5130.988 kb on - strand at 5130.988 kb on - strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5131.027 kb on + strand at 5131.046 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.119 kb on + strand at 5131.119 kb on + strand at 5131.120 kb on - strand at 5131.273 kb on - strand, within Psyr_4305 at 5131.408 kb on + strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.421 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.442 kb on + strand at 5131.443 kb on - strand at 5131.489 kb on + strand, within Psyr_4306 at 5131.604 kb on + strand, within Psyr_4306 at 5131.604 kb on + strand, within Psyr_4306 at 5131.605 kb on - strand, within Psyr_4306 at 5131.638 kb on - strand at 5131.638 kb on - strand at 5131.692 kb on + strand at 5131.693 kb on - strand at 5131.729 kb on + strand at 5131.730 kb on - strand at 5131.730 kb on - strand at 5131.731 kb on + strand at 5131.732 kb on - strand at 5131.732 kb on - strand at 5131.732 kb on - strand at 5131.732 kb on - strand at 5131.732 kb on - strand at 5131.759 kb on - strand at 5131.762 kb on + strand at 5131.763 kb on - strand at 5131.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cotarnine Chloride 10 mM remove 5,129,035 + +0.3 5,129,055 - +1.3 5,129,066 + +0.1 5,129,066 + +0.2 5,129,066 + -1.2 5,129,066 + -1.1 5,129,066 + -0.2 5,129,066 + -0.2 5,129,067 - -0.7 5,129,067 - +1.1 5,129,067 - -1.1 5,129,067 - +1.8 5,129,067 - +0.5 5,129,106 + +0.4 5,129,107 - +0.1 5,129,120 + -0.3 5,129,126 - +0.1 5,129,126 - +0.8 5,129,223 + Psyr_4303 0.23 -0.2 5,129,224 - Psyr_4303 0.23 +0.2 5,129,255 + Psyr_4303 0.27 +0.1 5,129,255 + Psyr_4303 0.27 +0.6 5,129,255 + Psyr_4303 0.27 -0.8 5,129,256 - Psyr_4303 0.28 -0.8 5,129,256 - Psyr_4303 0.28 +0.5 5,129,256 - Psyr_4303 0.28 +0.1 5,129,259 + Psyr_4303 0.28 +0.4 5,129,259 + Psyr_4303 0.28 -0.2 5,129,260 - Psyr_4303 0.28 -1.0 5,129,260 - Psyr_4303 0.28 +1.1 5,129,260 - Psyr_4303 0.28 -0.8 5,129,261 + Psyr_4303 0.28 +0.6 5,129,311 + Psyr_4303 0.36 -0.8 5,129,311 + Psyr_4303 0.36 +0.5 5,129,311 + Psyr_4303 0.36 -0.4 5,129,311 + Psyr_4303 0.36 +1.7 5,129,311 + Psyr_4303 0.36 -0.2 5,129,312 - Psyr_4303 0.36 +0.7 5,129,312 - Psyr_4303 0.36 +1.2 5,129,312 - Psyr_4303 0.36 +0.2 5,129,312 - Psyr_4303 0.36 +0.2 5,129,312 - Psyr_4303 0.36 +0.7 5,129,312 - Psyr_4303 0.36 -1.6 5,129,312 - Psyr_4303 0.36 +0.1 5,129,312 - Psyr_4303 0.36 -0.8 5,129,312 - Psyr_4303 0.36 +2.7 5,129,312 - Psyr_4303 0.36 +0.0 5,129,326 + Psyr_4303 0.38 -0.0 5,129,326 + Psyr_4303 0.38 -0.4 5,129,326 + Psyr_4303 0.38 +1.4 5,129,327 - Psyr_4303 0.38 +0.1 5,129,327 - Psyr_4303 0.38 -0.5 5,129,327 - Psyr_4303 0.38 +0.4 5,129,327 - Psyr_4303 0.38 -0.1 5,129,327 - Psyr_4303 0.38 +0.1 5,129,478 - Psyr_4303 0.61 +0.1 5,129,554 + Psyr_4303 0.73 +0.9 5,129,565 + Psyr_4303 0.74 -0.0 5,129,566 - Psyr_4303 0.74 -0.8 5,129,566 - Psyr_4303 0.74 +0.1 5,129,566 - Psyr_4303 0.74 -1.2 5,129,566 - Psyr_4303 0.74 +2.0 5,129,830 + +0.0 5,129,837 - -0.2 5,129,837 - -0.4 5,129,837 - +0.1 5,129,850 + +0.1 5,129,873 - +0.2 5,129,923 + Psyr_4304 0.13 -0.1 5,129,923 + Psyr_4304 0.13 -0.6 5,129,923 + Psyr_4304 0.13 +0.8 5,129,924 - Psyr_4304 0.14 -0.4 5,129,924 - Psyr_4304 0.14 +2.0 5,129,924 - Psyr_4304 0.14 -0.5 5,129,924 - Psyr_4304 0.14 -0.1 5,130,009 + Psyr_4304 0.21 +0.3 5,130,027 + Psyr_4304 0.23 +0.5 5,130,040 - Psyr_4304 0.24 +0.5 5,130,097 - Psyr_4304 0.29 -0.3 5,130,189 + Psyr_4304 0.37 +0.1 5,130,190 - Psyr_4304 0.37 +1.4 5,130,259 - Psyr_4304 0.43 +1.4 5,130,291 - Psyr_4304 0.46 -0.9 5,130,291 - Psyr_4304 0.46 -0.8 5,130,429 + Psyr_4304 0.58 +0.6 5,130,429 + Psyr_4304 0.58 -0.2 5,130,430 - Psyr_4304 0.58 -0.3 5,130,645 + Psyr_4304 0.77 -0.7 5,130,645 + Psyr_4304 0.77 -0.5 5,130,673 + Psyr_4304 0.79 -0.9 5,130,674 - Psyr_4304 0.79 +0.3 5,130,848 + +0.1 5,130,848 + +0.6 5,130,848 + -0.2 5,130,848 + +0.6 5,130,848 + -0.4 5,130,848 + +1.7 5,130,848 + -1.7 5,130,848 + +0.5 5,130,848 + +0.6 5,130,849 - -0.4 5,130,849 - +0.4 5,130,849 - -0.6 5,130,849 - +0.9 5,130,849 - +0.3 5,130,849 - +0.7 5,130,849 - +0.8 5,130,939 - -0.4 5,130,988 - -0.3 5,130,988 - +1.8 5,130,998 - +0.2 5,130,998 - -0.8 5,131,027 + +0.3 5,131,046 - +0.4 5,131,077 - -1.1 5,131,077 - +0.8 5,131,077 - +0.6 5,131,077 - -0.8 5,131,077 - -0.2 5,131,077 - -0.6 5,131,077 - +0.2 5,131,119 + -0.1 5,131,119 + +1.3 5,131,120 - -0.4 5,131,273 - Psyr_4305 0.71 -0.1 5,131,408 + +0.4 5,131,409 - +0.0 5,131,409 - +1.5 5,131,409 - +0.5 5,131,409 - -0.3 5,131,421 + +0.3 5,131,438 + +1.2 5,131,438 + +0.4 5,131,438 + +0.4 5,131,438 + -0.8 5,131,442 + -1.2 5,131,443 - +0.8 5,131,489 + Psyr_4306 0.20 -0.4 5,131,604 + Psyr_4306 0.78 -1.2 5,131,604 + Psyr_4306 0.78 -0.9 5,131,605 - Psyr_4306 0.79 -2.2 5,131,638 - -0.4 5,131,638 - +1.7 5,131,692 + +0.6 5,131,693 - +0.7 5,131,729 + -0.3 5,131,730 - -0.5 5,131,730 - +0.9 5,131,731 + +0.8 5,131,732 - +0.3 5,131,732 - -0.5 5,131,732 - -0.5 5,131,732 - +0.3 5,131,732 - +0.1 5,131,759 - -0.2 5,131,762 + +0.3 5,131,763 - -0.2 5,131,763 - -0.6
Or see this region's nucleotide sequence