Strain Fitness in Escherichia coli BW25113 around b4382

Experiment: LB Anaerobic with Spectinomycin 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdeoC and deoA are separated by 126 nucleotidesdeoA and deoB are separated by 51 nucleotides b4381: deoC - deoxyribose-phosphate aldolase (NCBI), at 4,615,346 to 4,616,125 deoC b4382: deoA - thymidine phosphorylase (NCBI), at 4,616,252 to 4,617,574 deoA b4383: deoB - phosphopentomutase (NCBI), at 4,617,626 to 4,618,849 deoB Position (kb) 4616 4617 4618Strain fitness (log2 ratio) -2 -1 0 1at 4615.261 kb on + strandat 4615.403 kb on - strandat 4615.505 kb on - strand, within deoCat 4615.505 kb on - strand, within deoCat 4615.724 kb on - strand, within deoCat 4615.727 kb on - strand, within deoCat 4615.734 kb on - strand, within deoCat 4615.750 kb on + strand, within deoCat 4615.829 kb on + strand, within deoCat 4615.829 kb on + strand, within deoCat 4615.837 kb on - strand, within deoCat 4616.063 kb on + strandat 4616.133 kb on + strandat 4616.148 kb on - strandat 4616.174 kb on + strandat 4616.198 kb on + strandat 4616.494 kb on - strand, within deoAat 4616.494 kb on - strand, within deoAat 4616.511 kb on - strand, within deoAat 4616.563 kb on - strand, within deoAat 4616.606 kb on - strand, within deoAat 4616.663 kb on + strand, within deoAat 4616.727 kb on - strand, within deoAat 4616.731 kb on + strand, within deoAat 4616.749 kb on + strand, within deoAat 4616.760 kb on + strand, within deoAat 4616.791 kb on - strand, within deoAat 4616.823 kb on - strand, within deoAat 4616.940 kb on - strand, within deoAat 4616.958 kb on - strand, within deoAat 4616.958 kb on - strand, within deoAat 4616.965 kb on + strand, within deoAat 4616.984 kb on + strand, within deoAat 4616.987 kb on - strand, within deoAat 4616.987 kb on - strand, within deoAat 4616.997 kb on + strand, within deoAat 4617.084 kb on - strand, within deoAat 4617.088 kb on + strand, within deoAat 4617.154 kb on - strand, within deoAat 4617.158 kb on + strand, within deoAat 4617.193 kb on - strand, within deoAat 4617.258 kb on + strand, within deoAat 4617.278 kb on + strand, within deoAat 4617.278 kb on + strand, within deoAat 4617.285 kb on - strand, within deoAat 4617.386 kb on - strand, within deoAat 4617.390 kb on - strand, within deoAat 4617.390 kb on - strand, within deoAat 4617.396 kb on + strand, within deoAat 4617.396 kb on + strand, within deoAat 4617.613 kb on + strandat 4617.631 kb on - strandat 4617.647 kb on + strandat 4617.647 kb on + strandat 4617.647 kb on + strandat 4617.655 kb on - strandat 4617.680 kb on - strandat 4617.691 kb on + strandat 4617.726 kb on - strandat 4617.861 kb on + strand, within deoBat 4617.869 kb on - strand, within deoBat 4617.891 kb on - strand, within deoBat 4617.961 kb on - strand, within deoBat 4618.009 kb on + strand, within deoBat 4618.009 kb on + strand, within deoBat 4618.012 kb on + strand, within deoBat 4618.012 kb on + strand, within deoBat 4618.030 kb on + strand, within deoBat 4618.030 kb on + strand, within deoBat 4618.076 kb on + strand, within deoBat 4618.088 kb on + strand, within deoBat 4618.285 kb on + strand, within deoBat 4618.335 kb on + strand, within deoBat 4618.411 kb on + strandat 4618.411 kb on + strand, within deoBat 4618.411 kb on + strand, within deoBat 4618.411 kb on + strand, within deoBat 4618.512 kb on - strand, within deoBat 4618.512 kb on - strand, within deoBat 4618.528 kb on + strand, within deoBat 4618.553 kb on - strand, within deoB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spectinomycin 0.0000625 mM
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4,615,261 + +0.9
4,615,403 - +0.5
4,615,505 - deoC b4381 0.20 +0.5
4,615,505 - deoC b4381 0.20 -0.4
4,615,724 - deoC b4381 0.48 -0.1
4,615,727 - deoC b4381 0.49 +0.0
4,615,734 - deoC b4381 0.50 -0.0
4,615,750 + deoC b4381 0.52 +0.7
4,615,829 + deoC b4381 0.62 -1.1
4,615,829 + deoC b4381 0.62 -0.3
4,615,837 - deoC b4381 0.63 -0.1
4,616,063 + +0.4
4,616,133 + +0.7
4,616,148 - +0.0
4,616,174 + +0.3
4,616,198 + +0.1
4,616,494 - deoA b4382 0.18 +1.5
4,616,494 - deoA b4382 0.18 +0.6
4,616,511 - deoA b4382 0.20 +0.9
4,616,563 - deoA b4382 0.24 +0.4
4,616,606 - deoA b4382 0.27 -0.1
4,616,663 + deoA b4382 0.31 +0.2
4,616,727 - deoA b4382 0.36 -0.2
4,616,731 + deoA b4382 0.36 +0.3
4,616,749 + deoA b4382 0.38 +1.4
4,616,760 + deoA b4382 0.38 +0.5
4,616,791 - deoA b4382 0.41 +1.7
4,616,823 - deoA b4382 0.43 +0.8
4,616,940 - deoA b4382 0.52 -0.1
4,616,958 - deoA b4382 0.53 -0.3
4,616,958 - deoA b4382 0.53 +0.2
4,616,965 + deoA b4382 0.54 +0.3
4,616,984 + deoA b4382 0.55 +0.6
4,616,987 - deoA b4382 0.56 +0.2
4,616,987 - deoA b4382 0.56 +0.1
4,616,997 + deoA b4382 0.56 +0.7
4,617,084 - deoA b4382 0.63 -0.1
4,617,088 + deoA b4382 0.63 +0.2
4,617,154 - deoA b4382 0.68 -0.0
4,617,158 + deoA b4382 0.68 +0.5
4,617,193 - deoA b4382 0.71 +0.6
4,617,258 + deoA b4382 0.76 +0.2
4,617,278 + deoA b4382 0.78 -0.1
4,617,278 + deoA b4382 0.78 +1.7
4,617,285 - deoA b4382 0.78 +0.5
4,617,386 - deoA b4382 0.86 +0.2
4,617,390 - deoA b4382 0.86 +1.4
4,617,390 - deoA b4382 0.86 -0.2
4,617,396 + deoA b4382 0.86 +0.5
4,617,396 + deoA b4382 0.86 +0.6
4,617,613 + +1.2
4,617,631 - -0.3
4,617,647 + -2.4
4,617,647 + -0.9
4,617,647 + -0.8
4,617,655 - -1.0
4,617,680 - -1.7
4,617,691 + +1.0
4,617,726 - -0.8
4,617,861 + deoB b4383 0.19 -2.1
4,617,869 - deoB b4383 0.20 -1.4
4,617,891 - deoB b4383 0.22 -1.6
4,617,961 - deoB b4383 0.27 -1.9
4,618,009 + deoB b4383 0.31 -1.0
4,618,009 + deoB b4383 0.31 -1.8
4,618,012 + deoB b4383 0.32 -1.4
4,618,012 + deoB b4383 0.32 -1.4
4,618,030 + deoB b4383 0.33 -0.5
4,618,030 + deoB b4383 0.33 -2.4
4,618,076 + deoB b4383 0.37 -0.4
4,618,088 + deoB b4383 0.38 -0.5
4,618,285 + deoB b4383 0.54 -0.7
4,618,335 + deoB b4383 0.58 -1.5
4,618,411 + +1.0
4,618,411 + deoB b4383 0.64 -2.0
4,618,411 + deoB b4383 0.64 -1.0
4,618,411 + deoB b4383 0.64 -2.0
4,618,512 - deoB b4383 0.72 -0.8
4,618,512 - deoB b4383 0.72 -1.1
4,618,528 + deoB b4383 0.74 +0.5
4,618,553 - deoB b4383 0.76 +0.0

Or see this region's nucleotide sequence