Strain Fitness in Escherichia coli BW25113 around b3477

Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
3,612,272	+	nikA	b3476	0.37	-0.4
3,612,403	+	nikA	b3476	0.45	-2.6
3,612,480	+	nikA	b3476	0.50	-2.0
3,612,480	+	nikA	b3476	0.50	+0.3
3,612,560	-	nikA	b3476	0.55	-1.4
3,612,560	-	nikA	b3476	0.55	-0.7
3,612,582	-	nikA	b3476	0.57	-1.3
3,612,602	+	nikA	b3476	0.58	-1.5
3,612,602	+	nikA	b3476	0.58	-2.5
3,612,612	-	nikA	b3476	0.59	-1.0
3,612,644	+	nikA	b3476	0.61	-0.7
3,612,666	+	nikA	b3476	0.62	-1.5
3,612,687	-	nikA	b3476	0.63	-1.6
3,612,701	+	nikA	b3476	0.64	-0.9
3,612,743	+	nikA	b3476	0.67	-1.2
3,612,801	-	nikA	b3476	0.71	-1.3
3,612,982	-	nikA	b3476	0.82	-0.5
3,613,011	+	nikA	b3476	0.84	-0.1
3,613,103	+	nikA	b3476	0.90	+0.0
3,613,103	+	nikA	b3476	0.90	-1.4
3,613,132	-				-1.1
3,613,165	-				-2.0
3,613,195	+				-1.0
3,613,211	-				-1.2
3,613,211	-				-1.0
3,613,251	-				+0.0
3,613,251	-				-2.0
3,613,252	+				-1.3
3,613,309	+				-0.7
3,613,309	+				-1.6
3,613,360	-	nikB	b3477	0.10	-1.5
3,613,403	+	nikB	b3477	0.15	+1.5
3,613,403	+	nikB	b3477	0.15	-2.6
3,613,445	+	nikB	b3477	0.19	-0.9
3,613,450	+	nikB	b3477	0.20	-1.2
3,613,461	-	nikB	b3477	0.21	-0.4
3,613,461	-	nikB	b3477	0.21	-3.0
3,613,467	-	nikB	b3477	0.21	+0.6
3,613,531	+	nikB	b3477	0.28	-2.0
3,613,539	-	nikB	b3477	0.29	-1.4
3,613,539	-	nikB	b3477	0.29	-1.9
3,613,539	-	nikB	b3477	0.29	-1.2
3,613,539	-	nikB	b3477	0.29	-0.5
3,613,539	-	nikB	b3477	0.29	-1.3
3,613,539	-	nikB	b3477	0.29	-1.0
3,613,539	-	nikB	b3477	0.29	-1.4
3,613,544	+	nikB	b3477	0.30	-0.3
3,613,551	-	nikB	b3477	0.30	-0.0
3,613,592	-	nikB	b3477	0.35	-0.1
3,613,607	+	nikB	b3477	0.36	-0.7
3,613,607	+	nikB	b3477	0.36	-0.8
3,613,607	+	nikB	b3477	0.36	-1.8
3,613,615	-	nikB	b3477	0.37	-0.9
3,613,615	-	nikB	b3477	0.37	-0.2
3,613,670	+	nikB	b3477	0.43	-0.6
3,613,670	+	nikB	b3477	0.43	-1.5
3,613,691	+	nikB	b3477	0.45	-1.7
3,613,773	-	nikB	b3477	0.54	-1.4
3,613,796	-	nikB	b3477	0.56	-2.1
3,613,848	-	nikB	b3477	0.62	-1.2
3,613,848	-	nikB	b3477	0.62	-0.7
3,613,869	+	nikB	b3477	0.64	-2.3
3,613,874	-	nikB	b3477	0.65	-1.0
3,613,878	+	nikB	b3477	0.65	-0.3
3,613,881	-	nikB	b3477	0.65	-0.9
3,613,932	+	nikB	b3477	0.71	-0.1
3,613,934	+	nikB	b3477	0.71	-0.9
3,613,942	+	nikB	b3477	0.72	-0.1
3,613,942	-	nikB	b3477	0.72	+0.0
3,613,947	-	nikB	b3477	0.72	-1.3
3,613,947	-	nikB	b3477	0.72	-1.3
3,613,988	+	nikB	b3477	0.77	-1.2
3,614,094	+	nikB	b3477	0.88	-2.3
3,614,249	+				+0.7
3,614,249	+				-0.0
3,614,272	+				-1.5
3,614,273	+				-0.3
3,614,286	+				-0.8
3,614,290	+	nikC	b3478	0.10	-3.0
3,614,316	-	nikC	b3478	0.13	-2.0
3,614,352	+	nikC	b3478	0.18	-1.4
3,614,371	-	nikC	b3478	0.20	-0.4
3,614,376	+	nikC	b3478	0.21	-1.6
3,614,432	-	nikC	b3478	0.27	-1.2
3,614,432	-	nikC	b3478	0.27	+0.1
3,614,436	+	nikC	b3478	0.28	-2.2
3,614,443	+	nikC	b3478	0.29	-0.6
3,614,443	+	nikC	b3478	0.29	-1.2
3,614,444	-	nikC	b3478	0.29	-0.4
3,614,446	+	nikC	b3478	0.29	-0.9
3,614,543	+	nikC	b3478	0.41	-0.1
3,614,551	-	nikC	b3478	0.41	-1.7
3,614,559	+	nikC	b3478	0.42	-1.9
3,614,639	-	nikC	b3478	0.52	-0.3
3,614,661	+	nikC	b3478	0.55	+0.1
3,614,661	+	nikC	b3478	0.55	-1.0
3,614,688	-	nikC	b3478	0.58	-0.9
3,614,688	-	nikC	b3478	0.58	-1.0
3,614,739	-	nikC	b3478	0.64	-0.1
3,614,739	-	nikC	b3478	0.64	-1.6
3,614,751	+	nikC	b3478	0.65	-0.7
3,614,753	-	nikC	b3478	0.66	-0.2
3,614,809	+	nikC	b3478	0.72	-0.1
3,614,868	+	nikC	b3478	0.79	-0.7
3,614,907	+	nikC	b3478	0.84	-1.0
3,614,911	-	nikC	b3478	0.85	-0.4
3,614,938	-	nikC	b3478	0.88	-0.6
3,614,983	+				+0.1
3,614,983	+				+0.2
3,615,008	-				-0.5
3,615,033	-				-0.9
3,615,047	-				-1.4
3,615,066	-				-1.2
3,615,089	+				-1.2
3,615,100	-				-1.2
3,615,103	+				-1.5
3,615,103	+				-0.6
3,615,164	+	nikD	b3479	0.16	-0.5