Strain Fitness in Escherichia coli BW25113 around b2730

Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypD and hypE overlap by 4 nucleotideshypE and fhlA are separated by 73 nucleotides b2729: hypD - protein required for maturation of hydrogenases (NCBI), at 2,850,158 to 2,851,279 hypD b2730: hypE - carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq), at 2,851,276 to 2,852,286 hypE b2731: fhlA - DNA-binding transcriptional activator (NCBI), at 2,852,360 to 2,854,438 fhlA Position (kb) 2851 2852 2853Strain fitness (log2 ratio) -2 -1 0 1 2at 2850.297 kb on - strand, within hypDat 2850.297 kb on - strand, within hypDat 2850.350 kb on - strand, within hypDat 2850.350 kb on - strand, within hypDat 2850.354 kb on + strand, within hypDat 2850.369 kb on - strand, within hypDat 2850.369 kb on - strand, within hypDat 2850.452 kb on + strand, within hypDat 2850.522 kb on - strand, within hypDat 2850.522 kb on - strand, within hypDat 2850.616 kb on - strand, within hypDat 2850.624 kb on - strand, within hypDat 2850.681 kb on + strand, within hypDat 2850.691 kb on - strand, within hypDat 2850.913 kb on - strand, within hypDat 2850.913 kb on - strand, within hypDat 2850.955 kb on - strand, within hypDat 2850.971 kb on + strand, within hypDat 2851.008 kb on + strand, within hypDat 2851.008 kb on + strand, within hypDat 2851.099 kb on + strand, within hypDat 2851.132 kb on + strand, within hypDat 2851.141 kb on - strand, within hypDat 2851.180 kb on + strandat 2851.180 kb on + strandat 2851.254 kb on - strandat 2851.318 kb on + strandat 2851.359 kb on - strandat 2851.359 kb on - strandat 2851.420 kb on - strand, within hypEat 2851.438 kb on - strand, within hypEat 2851.468 kb on + strand, within hypEat 2851.480 kb on + strand, within hypEat 2851.480 kb on + strand, within hypEat 2851.524 kb on - strand, within hypEat 2851.538 kb on + strand, within hypEat 2851.538 kb on + strand, within hypEat 2851.831 kb on - strand, within hypEat 2851.874 kb on + strand, within hypEat 2851.892 kb on - strand, within hypEat 2851.903 kb on + strand, within hypEat 2851.940 kb on - strand, within hypEat 2852.009 kb on - strand, within hypEat 2852.024 kb on + strand, within hypEat 2852.024 kb on + strand, within hypEat 2852.046 kb on - strand, within hypEat 2852.046 kb on - strand, within hypEat 2852.108 kb on - strand, within hypEat 2852.108 kb on - strand, within hypEat 2852.199 kb on - strandat 2852.199 kb on - strandat 2852.234 kb on - strandat 2852.234 kb on - strandat 2852.300 kb on + strandat 2852.300 kb on + strandat 2852.331 kb on + strandat 2852.331 kb on + strandat 2852.339 kb on - strandat 2852.364 kb on + strandat 2852.364 kb on + strandat 2852.428 kb on - strandat 2852.428 kb on - strandat 2852.457 kb on - strandat 2852.524 kb on + strandat 2852.612 kb on - strand, within fhlAat 2852.696 kb on - strand, within fhlAat 2852.796 kb on - strand, within fhlAat 2852.853 kb on + strand, within fhlAat 2852.866 kb on - strand, within fhlAat 2852.866 kb on - strand, within fhlAat 2852.896 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.990 kb on - strand, within fhlAat 2853.030 kb on + strand, within fhlAat 2853.167 kb on - strand, within fhlAat 2853.183 kb on + strand, within fhlAat 2853.183 kb on + strand, within fhlAat 2853.230 kb on - strand, within fhlAat 2853.251 kb on + strand, within fhlA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
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2,850,297 - hypD b2729 0.12 -0.4
2,850,297 - hypD b2729 0.12 -1.6
2,850,350 - hypD b2729 0.17 -0.3
2,850,350 - hypD b2729 0.17 -2.4
2,850,354 + hypD b2729 0.17 -0.8
2,850,369 - hypD b2729 0.19 -1.4
2,850,369 - hypD b2729 0.19 -1.2
2,850,452 + hypD b2729 0.26 -0.4
2,850,522 - hypD b2729 0.32 -0.3
2,850,522 - hypD b2729 0.32 +0.6
2,850,616 - hypD b2729 0.41 -1.5
2,850,624 - hypD b2729 0.42 -1.6
2,850,681 + hypD b2729 0.47 -1.0
2,850,691 - hypD b2729 0.48 -1.0
2,850,913 - hypD b2729 0.67 -1.0
2,850,913 - hypD b2729 0.67 -0.7
2,850,955 - hypD b2729 0.71 -1.4
2,850,971 + hypD b2729 0.72 -1.0
2,851,008 + hypD b2729 0.76 -0.6
2,851,008 + hypD b2729 0.76 -1.3
2,851,099 + hypD b2729 0.84 -0.7
2,851,132 + hypD b2729 0.87 -1.6
2,851,141 - hypD b2729 0.88 +0.8
2,851,180 + +0.0
2,851,180 + +0.4
2,851,254 - -0.4
2,851,318 + -0.6
2,851,359 - -1.9
2,851,359 - +0.0
2,851,420 - hypE b2730 0.14 -0.7
2,851,438 - hypE b2730 0.16 +0.6
2,851,468 + hypE b2730 0.19 -1.6
2,851,480 + hypE b2730 0.20 -0.7
2,851,480 + hypE b2730 0.20 -1.7
2,851,524 - hypE b2730 0.25 -2.1
2,851,538 + hypE b2730 0.26 -0.8
2,851,538 + hypE b2730 0.26 +0.4
2,851,831 - hypE b2730 0.55 +0.0
2,851,874 + hypE b2730 0.59 +0.4
2,851,892 - hypE b2730 0.61 -0.7
2,851,903 + hypE b2730 0.62 -1.4
2,851,940 - hypE b2730 0.66 +0.1
2,852,009 - hypE b2730 0.73 -1.3
2,852,024 + hypE b2730 0.74 -0.2
2,852,024 + hypE b2730 0.74 +0.0
2,852,046 - hypE b2730 0.76 -1.8
2,852,046 - hypE b2730 0.76 -0.2
2,852,108 - hypE b2730 0.82 -1.8
2,852,108 - hypE b2730 0.82 -0.8
2,852,199 - -0.6
2,852,199 - -1.8
2,852,234 - +1.0
2,852,234 - -1.0
2,852,300 + +0.5
2,852,300 + -1.1
2,852,331 + +1.8
2,852,331 + +1.0
2,852,339 - +0.7
2,852,364 + -0.7
2,852,364 + +1.0
2,852,428 - +0.0
2,852,428 - +0.6
2,852,457 - -0.2
2,852,524 + +0.2
2,852,612 - fhlA b2731 0.12 -0.5
2,852,696 - fhlA b2731 0.16 +0.5
2,852,796 - fhlA b2731 0.21 -1.1
2,852,853 + fhlA b2731 0.24 +0.5
2,852,866 - fhlA b2731 0.24 -1.0
2,852,866 - fhlA b2731 0.24 -0.3
2,852,896 + fhlA b2731 0.26 +0.2
2,852,962 + fhlA b2731 0.29 -0.6
2,852,962 + fhlA b2731 0.29 +0.1
2,852,962 + fhlA b2731 0.29 -0.2
2,852,990 - fhlA b2731 0.30 -0.6
2,853,030 + fhlA b2731 0.32 +0.0
2,853,167 - fhlA b2731 0.39 +0.1
2,853,183 + fhlA b2731 0.40 -1.3
2,853,183 + fhlA b2731 0.40 -0.8
2,853,230 - fhlA b2731 0.42 +0.2
2,853,251 + fhlA b2731 0.43 -1.1

Or see this region's nucleotide sequence