Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagA and yagE are separated by 294 nucleotides yagE and yagF are separated by 14 nucleotides
b0267: yagA - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 280,053 to 281,207
yagA
b0268: yagE - 2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6 prophage (NCBI), at 281,502 to 282,410
yagE
b0269: yagF - CP4-6 prophage; predicted dehydratase (NCBI), at 282,425 to 284,392
yagF
Position (kb) 281
282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 280.581 kb on + strand, within yagA at 280.715 kb on + strand, within yagA at 280.940 kb on + strand, within yagA at 280.955 kb on + strand, within yagA at 280.955 kb on + strand, within yagA at 280.973 kb on - strand, within yagA at 281.054 kb on - strand, within yagA at 281.054 kb on - strand, within yagA at 281.236 kb on + strand at 281.236 kb on + strand at 281.271 kb on + strand at 281.293 kb on - strand at 281.293 kb on - strand at 281.359 kb on - strand at 281.359 kb on - strand at 281.464 kb on + strand at 281.464 kb on + strand at 281.579 kb on + strand at 281.579 kb on + strand at 281.639 kb on - strand, within yagE at 281.664 kb on + strand, within yagE at 281.869 kb on + strand, within yagE at 282.021 kb on - strand, within yagE at 282.021 kb on - strand, within yagE at 282.072 kb on + strand, within yagE at 282.101 kb on + strand, within yagE at 282.109 kb on - strand, within yagE at 282.109 kb on - strand, within yagE at 282.114 kb on - strand, within yagE at 282.114 kb on - strand, within yagE at 282.297 kb on + strand, within yagE at 282.305 kb on + strand, within yagE at 282.321 kb on - strand at 282.321 kb on - strand at 282.449 kb on + strand at 282.524 kb on - strand at 282.524 kb on - strand at 282.553 kb on + strand at 282.591 kb on + strand at 282.684 kb on - strand, within yagF at 282.811 kb on + strand, within yagF at 282.811 kb on + strand, within yagF at 282.958 kb on - strand, within yagF at 283.075 kb on - strand, within yagF at 283.090 kb on - strand, within yagF at 283.154 kb on - strand, within yagF at 283.393 kb on + strand, within yagF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM remove 280,581 + yagA b0267 0.46 -1.2 280,715 + yagA b0267 0.57 -0.0 280,940 + yagA b0267 0.77 +0.4 280,955 + yagA b0267 0.78 +0.2 280,955 + yagA b0267 0.78 -0.9 280,973 - yagA b0267 0.80 +0.6 281,054 - yagA b0267 0.87 -0.0 281,054 - yagA b0267 0.87 +0.2 281,236 + -0.5 281,236 + -1.0 281,271 + +0.4 281,293 - -0.8 281,293 - +1.2 281,359 - +0.4 281,359 - -0.0 281,464 + -0.8 281,464 + +0.1 281,579 + +0.3 281,579 + +1.1 281,639 - yagE b0268 0.15 -1.0 281,664 + yagE b0268 0.18 -0.2 281,869 + yagE b0268 0.40 -0.7 282,021 - yagE b0268 0.57 -1.5 282,021 - yagE b0268 0.57 +0.1 282,072 + yagE b0268 0.63 +0.2 282,101 + yagE b0268 0.66 -0.0 282,109 - yagE b0268 0.67 -0.9 282,109 - yagE b0268 0.67 -1.4 282,114 - yagE b0268 0.67 -1.5 282,114 - yagE b0268 0.67 -1.9 282,297 + yagE b0268 0.87 -0.1 282,305 + yagE b0268 0.88 -0.8 282,321 - -1.4 282,321 - +0.3 282,449 + +0.4 282,524 - +0.5 282,524 - +0.8 282,553 + +0.4 282,591 + +0.5 282,684 - yagF b0269 0.13 -3.1 282,811 + yagF b0269 0.20 -0.9 282,811 + yagF b0269 0.20 +0.8 282,958 - yagF b0269 0.27 -0.6 283,075 - yagF b0269 0.33 +0.4 283,090 - yagF b0269 0.34 -0.6 283,154 - yagF b0269 0.37 +0.8 283,393 + yagF b0269 0.49 +0.9
Or see this region's nucleotide sequence