Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2758 and Psyr_2759 are separated by 89 nucleotides Psyr_2759 and Psyr_RNA43 are separated by 167 nucleotides Psyr_RNA43 and Psyr_2760 are separated by 186 nucleotides Psyr_2760 and Psyr_2761 are separated by 46 nucleotides
Psyr_2758: Psyr_2758 - ABC transporter:TOBE, at 3,345,625 to 3,346,713
_2758
Psyr_2759: Psyr_2759 - Eukaryotic-like DNA topoisomerase I, at 3,346,803 to 3,347,873
_2759
Psyr_RNA43: Psyr_RNA43 - tRNA-Leu, at 3,348,041 to 3,348,126
_RNA43
Psyr_2760: Psyr_2760 - hypothetical protein, at 3,348,313 to 3,348,675
_2760
Psyr_2761: Psyr_2761 - DNA-directed DNA polymerase, at 3,348,722 to 3,350,002
_2761
Position (kb)
3346
3347
3348 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3345.854 kb on - strand, within Psyr_2758 at 3345.871 kb on + strand, within Psyr_2758 at 3345.871 kb on + strand, within Psyr_2758 at 3345.872 kb on - strand, within Psyr_2758 at 3345.872 kb on - strand, within Psyr_2758 at 3346.153 kb on + strand, within Psyr_2758 at 3346.153 kb on + strand, within Psyr_2758 at 3346.195 kb on + strand, within Psyr_2758 at 3346.195 kb on + strand, within Psyr_2758 at 3346.196 kb on - strand, within Psyr_2758 at 3346.196 kb on - strand, within Psyr_2758 at 3346.196 kb on - strand, within Psyr_2758 at 3346.265 kb on + strand, within Psyr_2758 at 3346.266 kb on - strand, within Psyr_2758 at 3346.366 kb on + strand, within Psyr_2758 at 3346.379 kb on - strand, within Psyr_2758 at 3346.758 kb on + strand at 3346.759 kb on - strand at 3346.798 kb on + strand at 3346.799 kb on - strand at 3346.848 kb on + strand at 3346.849 kb on - strand at 3346.849 kb on - strand at 3346.849 kb on - strand at 3346.849 kb on - strand at 3346.849 kb on - strand at 3346.853 kb on - strand at 3347.067 kb on + strand, within Psyr_2759 at 3347.089 kb on + strand, within Psyr_2759 at 3347.089 kb on + strand, within Psyr_2759 at 3347.090 kb on - strand, within Psyr_2759 at 3347.107 kb on - strand, within Psyr_2759 at 3347.107 kb on - strand, within Psyr_2759 at 3347.259 kb on + strand, within Psyr_2759 at 3347.468 kb on - strand, within Psyr_2759 at 3347.694 kb on + strand, within Psyr_2759 at 3347.694 kb on + strand, within Psyr_2759 at 3347.802 kb on + strand at 3347.802 kb on + strand at 3347.803 kb on - strand at 3347.812 kb on + strand at 3348.014 kb on + strand at 3348.014 kb on + strand at 3348.014 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.018 kb on + strand at 3348.187 kb on + strand at 3348.188 kb on - strand at 3348.191 kb on + strand at 3348.191 kb on + strand at 3348.191 kb on + strand at 3348.192 kb on - strand at 3348.192 kb on - strand at 3348.192 kb on - strand at 3348.277 kb on - strand at 3348.290 kb on - strand at 3348.402 kb on + strand, within Psyr_2760 at 3348.402 kb on + strand, within Psyr_2760 at 3348.403 kb on - strand, within Psyr_2760 at 3348.403 kb on - strand, within Psyr_2760 at 3348.422 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.590 kb on + strand, within Psyr_2760 at 3348.635 kb on + strand, within Psyr_2760 at 3348.765 kb on + strand at 3348.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 3,345,854 - Psyr_2758 0.21 +1.1 3,345,871 + Psyr_2758 0.23 -1.4 3,345,871 + Psyr_2758 0.23 -0.4 3,345,872 - Psyr_2758 0.23 +0.2 3,345,872 - Psyr_2758 0.23 +0.8 3,346,153 + Psyr_2758 0.48 -0.6 3,346,153 + Psyr_2758 0.48 -0.3 3,346,195 + Psyr_2758 0.52 +0.2 3,346,195 + Psyr_2758 0.52 +0.2 3,346,196 - Psyr_2758 0.52 +0.7 3,346,196 - Psyr_2758 0.52 +1.3 3,346,196 - Psyr_2758 0.52 +0.8 3,346,265 + Psyr_2758 0.59 -2.5 3,346,266 - Psyr_2758 0.59 +1.1 3,346,366 + Psyr_2758 0.68 +0.6 3,346,379 - Psyr_2758 0.69 -0.0 3,346,758 + -0.5 3,346,759 - -2.3 3,346,798 + -1.5 3,346,799 - -2.0 3,346,848 + +0.4 3,346,849 - -0.4 3,346,849 - -1.2 3,346,849 - +1.2 3,346,849 - +0.6 3,346,849 - -0.0 3,346,853 - +1.1 3,347,067 + Psyr_2759 0.25 -0.6 3,347,089 + Psyr_2759 0.27 +0.2 3,347,089 + Psyr_2759 0.27 -1.5 3,347,090 - Psyr_2759 0.27 +0.3 3,347,107 - Psyr_2759 0.28 -1.6 3,347,107 - Psyr_2759 0.28 +2.1 3,347,259 + Psyr_2759 0.43 +0.3 3,347,468 - Psyr_2759 0.62 -0.4 3,347,694 + Psyr_2759 0.83 -0.9 3,347,694 + Psyr_2759 0.83 +2.1 3,347,802 + -0.2 3,347,802 + -1.0 3,347,803 - -0.7 3,347,812 + +1.6 3,348,014 + +0.1 3,348,014 + +0.5 3,348,014 + +0.3 3,348,016 + +0.3 3,348,016 + -0.3 3,348,016 + +0.4 3,348,016 + +1.2 3,348,016 + -1.3 3,348,016 + +3.1 3,348,018 + -2.3 3,348,187 + +0.1 3,348,188 - -0.1 3,348,191 + -0.0 3,348,191 + -0.5 3,348,191 + -0.3 3,348,192 - -0.9 3,348,192 - -0.6 3,348,192 - +0.2 3,348,277 - -0.3 3,348,290 - -0.9 3,348,402 + Psyr_2760 0.25 +0.5 3,348,402 + Psyr_2760 0.25 +0.3 3,348,403 - Psyr_2760 0.25 -0.8 3,348,403 - Psyr_2760 0.25 +0.7 3,348,422 + Psyr_2760 0.30 +1.8 3,348,531 + Psyr_2760 0.60 -1.1 3,348,531 + Psyr_2760 0.60 +0.2 3,348,531 + Psyr_2760 0.60 +1.1 3,348,531 + Psyr_2760 0.60 -0.0 3,348,531 + Psyr_2760 0.60 -0.5 3,348,531 + Psyr_2760 0.60 +0.5 3,348,531 + Psyr_2760 0.60 +0.6 3,348,532 - Psyr_2760 0.60 +0.4 3,348,532 - Psyr_2760 0.60 -1.4 3,348,532 - Psyr_2760 0.60 +0.7 3,348,590 + Psyr_2760 0.76 -0.2 3,348,635 + Psyr_2760 0.89 -0.8 3,348,765 + -0.2 3,348,765 + +0.1
Or see this region's nucleotide sequence