Experiment: KB with Cantharidin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0820 and Psyr_0821 are separated by 355 nucleotides Psyr_0821 and Psyr_0822 overlap by 1 nucleotides Psyr_0822 and Psyr_0823 are separated by 12 nucleotides
Psyr_0820: Psyr_0820 - transcriptional regulator, LacI family, at 925,431 to 926,426
_0820
Psyr_0821: Psyr_0821 - PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase, at 926,782 to 929,652
_0821
Psyr_0822: Psyr_0822 - 1-phosphofructokinase, at 929,652 to 930,593
_0822
Psyr_0823: Psyr_0823 - PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family, at 930,606 to 932,348
_0823
Position (kb) 926
927
928
929
930 Strain fitness (log2 ratio) -2
-1
0
1
2 at 926.162 kb on + strand, within Psyr_0820 at 926.162 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.247 kb on - strand, within Psyr_0820 at 926.340 kb on - strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.372 kb on - strand at 926.442 kb on + strand at 926.443 kb on - strand at 926.443 kb on - strand at 926.445 kb on + strand at 926.445 kb on + strand at 926.446 kb on - strand at 926.446 kb on - strand at 926.446 kb on - strand at 926.446 kb on - strand at 926.496 kb on + strand at 926.523 kb on + strand at 926.523 kb on + strand at 926.524 kb on - strand at 926.533 kb on - strand at 926.533 kb on - strand at 926.538 kb on - strand at 926.691 kb on - strand at 926.691 kb on - strand at 926.735 kb on - strand at 926.735 kb on - strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.848 kb on - strand at 926.971 kb on - strand at 926.971 kb on - strand at 927.194 kb on + strand, within Psyr_0821 at 927.194 kb on + strand, within Psyr_0821 at 927.195 kb on - strand, within Psyr_0821 at 927.195 kb on - strand, within Psyr_0821 at 927.195 kb on - strand, within Psyr_0821 at 927.195 kb on - strand, within Psyr_0821 at 927.451 kb on + strand, within Psyr_0821 at 927.452 kb on - strand, within Psyr_0821 at 927.452 kb on - strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.440 kb on - strand, within Psyr_0821 at 928.613 kb on + strand, within Psyr_0821 at 928.614 kb on - strand, within Psyr_0821 at 928.682 kb on + strand, within Psyr_0821 at 928.960 kb on - strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.650 kb on + strand at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.783 kb on - strand, within Psyr_0822 at 929.928 kb on - strand, within Psyr_0822 at 930.571 kb on + strand at 930.572 kb on - strand at 930.591 kb on + strand at 930.591 kb on + strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.623 kb on + strand at 930.623 kb on + strand at 930.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cantharidin 10 mM remove 926,162 + Psyr_0820 0.73 +0.6 926,162 + Psyr_0820 0.73 -0.5 926,200 + Psyr_0820 0.77 -1.2 926,200 + Psyr_0820 0.77 -0.9 926,200 + Psyr_0820 0.77 +0.5 926,200 + Psyr_0820 0.77 -0.2 926,200 + Psyr_0820 0.77 -0.3 926,201 - Psyr_0820 0.77 +0.2 926,201 - Psyr_0820 0.77 +0.2 926,246 + Psyr_0820 0.82 -0.0 926,246 + Psyr_0820 0.82 +0.0 926,247 - Psyr_0820 0.82 -0.1 926,340 - -1.0 926,371 + -1.3 926,371 + +1.0 926,371 + +0.9 926,371 + -2.3 926,372 - +0.1 926,442 + +0.1 926,443 - -0.3 926,443 - +0.5 926,445 + -0.2 926,445 + -0.1 926,446 - -0.4 926,446 - -0.3 926,446 - +0.8 926,446 - +0.7 926,496 + -0.8 926,523 + -0.9 926,523 + +0.3 926,524 - -0.1 926,533 - +0.3 926,533 - -0.0 926,538 - -0.1 926,691 - -0.8 926,691 - +0.3 926,735 - -1.0 926,735 - +0.4 926,847 + +0.9 926,847 + -0.2 926,847 + -0.2 926,848 - -0.8 926,971 - +0.3 926,971 - -0.7 927,194 + Psyr_0821 0.14 +0.7 927,194 + Psyr_0821 0.14 +1.7 927,195 - Psyr_0821 0.14 -0.3 927,195 - Psyr_0821 0.14 -0.3 927,195 - Psyr_0821 0.14 +0.0 927,195 - Psyr_0821 0.14 -0.7 927,451 + Psyr_0821 0.23 -2.0 927,452 - Psyr_0821 0.23 +0.8 927,452 - Psyr_0821 0.23 +0.7 928,429 + Psyr_0821 0.57 +0.6 928,429 + Psyr_0821 0.57 +0.1 928,430 - Psyr_0821 0.57 -0.1 928,430 - Psyr_0821 0.57 -0.4 928,430 - Psyr_0821 0.57 -0.8 928,430 - Psyr_0821 0.57 +0.1 928,430 - Psyr_0821 0.57 -0.9 928,430 - Psyr_0821 0.57 +1.1 928,440 - Psyr_0821 0.58 +0.8 928,613 + Psyr_0821 0.64 -0.0 928,614 - Psyr_0821 0.64 -0.5 928,682 + Psyr_0821 0.66 +1.0 928,960 - Psyr_0821 0.76 +0.5 929,320 + Psyr_0821 0.88 +0.3 929,320 + Psyr_0821 0.88 +2.6 929,321 - Psyr_0821 0.88 +0.2 929,321 - Psyr_0821 0.88 -0.3 929,321 - Psyr_0821 0.88 +0.1 929,650 + -0.0 929,782 + Psyr_0822 0.14 +0.2 929,782 + Psyr_0822 0.14 -2.0 929,782 + Psyr_0822 0.14 -0.7 929,782 + Psyr_0822 0.14 +0.1 929,782 + Psyr_0822 0.14 -0.7 929,782 + Psyr_0822 0.14 +0.3 929,783 - Psyr_0822 0.14 +0.7 929,928 - Psyr_0822 0.29 +0.0 930,571 + +0.8 930,572 - +1.2 930,591 + +0.9 930,591 + +0.5 930,592 - -0.2 930,592 - -0.6 930,619 + -0.3 930,619 + -1.6 930,619 + -0.2 930,620 - -0.1 930,620 - +0.3 930,620 - +1.1 930,623 + +0.3 930,623 + +0.5 930,624 - -0.8
Or see this region's nucleotide sequence