Experiment: LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yceG and tmk overlap by 11 nucleotides tmk and holB overlap by 4 nucleotides holB and ycfH are separated by 10 nucleotides
b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357
yceG
b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988
tmk
b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989
holB
b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797
ycfH
Position (kb)
1154
1155
1156 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1154.104 kb on - strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.160 kb on - strand, within yceG at 1154.280 kb on - strand at 1154.323 kb on + strand at 1154.323 kb on + strand at 1155.056 kb on + strand at 1155.960 kb on + strand at 1155.960 kb on + strand at 1155.966 kb on + strand at 1156.034 kb on - strand at 1156.056 kb on + strand at 1156.056 kb on + strand at 1156.065 kb on + strand at 1156.078 kb on - strand at 1156.157 kb on + strand, within ycfH at 1156.157 kb on + strand, within ycfH at 1156.258 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.318 kb on - strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.358 kb on + strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.392 kb on - strand, within ycfH at 1156.456 kb on + strand, within ycfH at 1156.484 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.513 kb on - strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.568 kb on + strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.598 kb on - strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.602 kb on + strand, within ycfH at 1156.617 kb on + strand, within ycfH at 1156.622 kb on + strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.636 kb on - strand, within ycfH at 1156.686 kb on + strand, within ycfH at 1156.692 kb on - strand, within ycfH at 1156.696 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.698 kb on + strand, within ycfH at 1156.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM remove 1,154,104 - yceG b1097 0.75 +0.8 1,154,150 + yceG b1097 0.80 -0.1 1,154,150 + yceG b1097 0.80 +0.9 1,154,152 - yceG b1097 0.80 +2.2 1,154,152 - yceG b1097 0.80 -0.4 1,154,158 - yceG b1097 0.80 -0.2 1,154,158 - yceG b1097 0.80 -1.5 1,154,160 - yceG b1097 0.81 -0.5 1,154,280 - -0.8 1,154,323 + -1.2 1,154,323 + -0.8 1,155,056 + -0.2 1,155,960 + +0.2 1,155,960 + +0.8 1,155,966 + -0.1 1,156,034 - +0.6 1,156,056 + +0.1 1,156,056 + -0.3 1,156,065 + +0.6 1,156,078 - -0.4 1,156,157 + ycfH b1100 0.20 -1.5 1,156,157 + ycfH b1100 0.20 +0.1 1,156,258 - ycfH b1100 0.32 +0.4 1,156,318 - ycfH b1100 0.40 -0.6 1,156,318 - ycfH b1100 0.40 -0.2 1,156,358 + ycfH b1100 0.45 -2.0 1,156,358 + ycfH b1100 0.45 -0.3 1,156,392 - ycfH b1100 0.49 -1.2 1,156,392 - ycfH b1100 0.49 -0.2 1,156,392 - ycfH b1100 0.49 +0.9 1,156,392 - ycfH b1100 0.49 -1.5 1,156,456 + ycfH b1100 0.57 +0.1 1,156,484 - ycfH b1100 0.61 +0.2 1,156,513 - ycfH b1100 0.64 -0.8 1,156,513 - ycfH b1100 0.64 +0.4 1,156,568 + ycfH b1100 0.71 -2.2 1,156,568 + ycfH b1100 0.71 +0.3 1,156,598 - ycfH b1100 0.75 -0.2 1,156,598 - ycfH b1100 0.75 -0.2 1,156,602 + ycfH b1100 0.75 +0.3 1,156,602 + ycfH b1100 0.75 +1.3 1,156,602 + ycfH b1100 0.75 -0.2 1,156,617 + ycfH b1100 0.77 +0.0 1,156,622 + ycfH b1100 0.78 +0.6 1,156,636 - ycfH b1100 0.80 -0.4 1,156,636 - ycfH b1100 0.80 -1.1 1,156,686 + ycfH b1100 0.86 +0.2 1,156,692 - ycfH b1100 0.87 +0.5 1,156,696 + ycfH b1100 0.87 -0.1 1,156,698 + ycfH b1100 0.87 +0.8 1,156,698 + ycfH b1100 0.87 -0.4 1,156,698 + ycfH b1100 0.87 +0.8 1,156,731 + +0.4
Or see this region's nucleotide sequence