Experiment: Growth on radish seeds for 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04515 and PS417_04520 are separated by 134 nucleotides PS417_04520 and PS417_04525 are separated by 260 nucleotides PS417_04525 and PS417_04530 are separated by 30 nucleotides
PS417_04515: PS417_04515 - peptidase M48, at 997,720 to 998,538
_04515
PS417_04520: PS417_04520 - membrane protein, at 998,673 to 999,257
_04520
PS417_04525: PS417_04525 - 16S rRNA methyltransferase, at 999,518 to 1,000,516
_04525
PS417_04530: PS417_04530 - glycerate dehydrogenase, at 1,000,547 to 1,001,512
_04530
Position (kb)
999
1000
1001 Strain fitness (log2 ratio)
-1
0
1
2
3 at 998.543 kb on - strand at 998.562 kb on - strand at 998.608 kb on - strand at 998.608 kb on - strand at 998.674 kb on + strand at 998.674 kb on + strand at 998.675 kb on - strand at 998.675 kb on - strand at 998.704 kb on + strand at 998.705 kb on - strand at 998.705 kb on - strand at 998.705 kb on - strand at 999.262 kb on + strand at 999.263 kb on - strand at 999.335 kb on + strand at 999.335 kb on + strand at 999.336 kb on - strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.447 kb on + strand at 999.448 kb on - strand at 999.735 kb on - strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 1000.371 kb on - strand, within PS417_04525 at 1000.496 kb on + strand at 1000.497 kb on - strand at 1000.497 kb on - strand at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.724 kb on + strand, within PS417_04530 at 1001.140 kb on - strand, within PS417_04530
Per-strain Table
Position Strand Gene LocusTag Fraction Growth on radish seeds for 24 hours remove 998,543 - -0.2 998,562 - +0.9 998,608 - -0.6 998,608 - -0.6 998,674 + +0.3 998,674 + -0.4 998,675 - +1.1 998,675 - -1.2 998,704 + +0.4 998,705 - -0.3 998,705 - -0.6 998,705 - -1.0 999,262 + +0.5 999,263 - +1.1 999,335 + +1.1 999,335 + +0.7 999,336 - +2.1 999,405 + +2.5 999,405 + +2.5 999,405 + -0.6 999,405 + +0.6 999,406 - -0.9 999,406 - -0.1 999,406 - +1.1 999,406 - +0.5 999,447 + -0.2 999,448 - -0.4 999,735 - PS417_04525 0.22 -0.0 999,854 + PS417_04525 0.34 -1.3 999,854 + PS417_04525 0.34 +1.0 999,855 - PS417_04525 0.34 +1.1 999,855 - PS417_04525 0.34 +1.6 999,855 - PS417_04525 0.34 +0.1 999,855 - PS417_04525 0.34 +3.0 999,855 - PS417_04525 0.34 -0.2 1,000,371 - PS417_04525 0.85 +2.1 1,000,496 + -0.1 1,000,497 - -1.4 1,000,497 - +0.5 1,000,715 + PS417_04530 0.17 +0.6 1,000,715 + PS417_04530 0.17 -0.2 1,000,715 + PS417_04530 0.17 +1.7 1,000,724 + PS417_04530 0.18 +0.6 1,001,140 - PS417_04530 0.61 +3.1
Or see this region's nucleotide sequence