Experiment: Growth on radish seeds for 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10395 and PS417_10400 are separated by 226 nucleotides PS417_10400 and PS417_10405 overlap by 4 nucleotides PS417_10405 and PS417_10410 are separated by 91 nucleotides PS417_10410 and PS417_10415 are separated by 225 nucleotides
PS417_10395: PS417_10395 - amidase, at 2,223,139 to 2,223,918
_10395
PS417_10400: PS417_10400 - membrane protein, at 2,224,145 to 2,225,212
_10400
PS417_10405: PS417_10405 - filamentous hemagglutinin, at 2,225,209 to 2,225,379
_10405
PS417_10410: PS417_10410 - hypothetical protein, at 2,225,471 to 2,225,665
_10410
PS417_10415: PS417_10415 - hypothetical protein, at 2,225,891 to 2,226,265
_10415
Position (kb)
2224
2225
2226 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2223.192 kb on + strand at 2223.357 kb on + strand, within PS417_10395 at 2223.358 kb on - strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395 at 2223.703 kb on + strand, within PS417_10395 at 2223.704 kb on - strand, within PS417_10395 at 2223.910 kb on + strand at 2223.911 kb on - strand at 2224.050 kb on - strand at 2224.050 kb on - strand at 2224.050 kb on - strand at 2224.095 kb on + strand at 2224.109 kb on + strand at 2224.151 kb on - strand at 2224.490 kb on + strand, within PS417_10400 at 2224.652 kb on + strand, within PS417_10400 at 2224.653 kb on - strand, within PS417_10400 at 2224.662 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.941 kb on - strand, within PS417_10400 at 2225.259 kb on + strand at 2225.259 kb on + strand, within PS417_10405 at 2225.410 kb on + strand at 2225.428 kb on - strand at 2225.759 kb on + strand at 2225.760 kb on - strand at 2225.760 kb on - strand at 2225.949 kb on + strand, within PS417_10415 at 2225.950 kb on - strand, within PS417_10415 at 2225.973 kb on + strand, within PS417_10415 at 2225.974 kb on - strand, within PS417_10415 at 2225.974 kb on - strand at 2225.974 kb on - strand, within PS417_10415 at 2225.994 kb on + strand, within PS417_10415 at 2225.995 kb on - strand, within PS417_10415 at 2226.012 kb on + strand, within PS417_10415 at 2226.012 kb on + strand, within PS417_10415 at 2226.013 kb on - strand, within PS417_10415 at 2226.013 kb on - strand, within PS417_10415 at 2226.037 kb on - strand, within PS417_10415 at 2226.078 kb on + strand, within PS417_10415 at 2226.078 kb on + strand, within PS417_10415 at 2226.079 kb on - strand, within PS417_10415 at 2226.079 kb on - strand, within PS417_10415 at 2226.097 kb on - strand, within PS417_10415
Per-strain Table
Position Strand Gene LocusTag Fraction Growth on radish seeds for 24 hours remove 2,223,192 + +0.3 2,223,357 + PS417_10395 0.28 -0.5 2,223,358 - PS417_10395 0.28 +0.4 2,223,545 + PS417_10395 0.52 -1.0 2,223,545 + PS417_10395 0.52 -1.4 2,223,546 - PS417_10395 0.52 +0.4 2,223,546 - PS417_10395 0.52 -0.1 2,223,703 + PS417_10395 0.72 +0.1 2,223,704 - PS417_10395 0.72 -0.1 2,223,910 + -0.4 2,223,911 - +0.6 2,224,050 - -0.4 2,224,050 - +1.6 2,224,050 - -1.0 2,224,095 + +0.3 2,224,109 + -1.4 2,224,151 - +1.3 2,224,490 + PS417_10400 0.32 -0.4 2,224,652 + PS417_10400 0.47 -1.2 2,224,653 - PS417_10400 0.48 -1.6 2,224,662 - PS417_10400 0.48 +0.6 2,224,697 - PS417_10400 0.52 +1.2 2,224,697 - PS417_10400 0.52 -2.0 2,224,697 - PS417_10400 0.52 -0.4 2,224,941 - PS417_10400 0.75 -0.4 2,225,259 + +1.6 2,225,259 + PS417_10405 0.29 -0.5 2,225,410 + -0.4 2,225,428 - +0.9 2,225,759 + +0.3 2,225,760 - +0.4 2,225,760 - +0.1 2,225,949 + PS417_10415 0.15 -0.8 2,225,950 - PS417_10415 0.16 -0.8 2,225,973 + PS417_10415 0.22 +1.6 2,225,974 - PS417_10415 0.22 -1.4 2,225,974 - -1.0 2,225,974 - PS417_10415 0.22 +1.2 2,225,994 + PS417_10415 0.27 +0.4 2,225,995 - PS417_10415 0.28 +1.0 2,226,012 + PS417_10415 0.32 -0.4 2,226,012 + PS417_10415 0.32 -0.7 2,226,013 - PS417_10415 0.33 -1.0 2,226,013 - PS417_10415 0.33 -0.1 2,226,037 - PS417_10415 0.39 +0.5 2,226,078 + PS417_10415 0.50 +1.5 2,226,078 + PS417_10415 0.50 +2.1 2,226,079 - PS417_10415 0.50 -1.1 2,226,079 - PS417_10415 0.50 +0.4 2,226,097 - PS417_10415 0.55 -0.1
Or see this region's nucleotide sequence