Experiment: LB Anaerobic with Cefoxitin 0.000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sfsA and yadP are separated by 14 nucleotides yadP and hrpB are separated by 73 nucleotides hrpB and mrcB are separated by 195 nucleotides
b0146: sfsA - sugar fermentation stimulation protein A (NCBI), at 160,782 to 161,486
sfsA
b0147: yadP - orf, hypothetical protein (VIMSS), at 161,501 to 162,031
yadP
b0148: hrpB - helicase, ATP-dependent (VIMSS), at 162,105 to 164,534
hrpB
b0149: mrcB - penicillin-binding protein 1b (NCBI), at 164,730 to 167,264
mrcB
Position (kb) 162
163
164
165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 161.141 kb on - strand, within sfsA at 161.146 kb on - strand, within sfsA at 161.202 kb on + strand, within sfsA at 161.202 kb on + strand, within sfsA at 161.215 kb on - strand, within sfsA at 161.351 kb on + strand, within sfsA at 161.375 kb on + strand, within sfsA at 161.375 kb on + strand, within sfsA at 161.385 kb on + strand, within sfsA at 161.385 kb on + strand, within sfsA at 161.393 kb on - strand, within sfsA at 161.393 kb on - strand, within sfsA at 161.393 kb on - strand, within sfsA at 161.393 kb on - strand, within sfsA at 161.398 kb on - strand, within sfsA at 161.418 kb on - strand at 161.418 kb on - strand at 161.437 kb on + strand at 161.463 kb on + strand at 161.463 kb on + strand at 161.463 kb on + strand at 161.463 kb on + strand at 161.471 kb on - strand at 161.471 kb on - strand at 161.471 kb on - strand at 161.471 kb on - strand at 161.529 kb on + strand at 161.529 kb on + strand at 161.532 kb on + strand at 161.545 kb on - strand at 161.564 kb on + strand, within yadP at 161.600 kb on + strand, within yadP at 161.652 kb on + strand, within yadP at 161.659 kb on - strand, within yadP at 161.714 kb on + strand, within yadP at 161.758 kb on + strand, within yadP at 161.758 kb on + strand, within yadP at 161.758 kb on + strand, within yadP at 161.780 kb on + strand, within yadP at 161.780 kb on + strand, within yadP at 161.791 kb on + strand, within yadP at 161.804 kb on - strand, within yadP at 161.854 kb on + strand, within yadP at 161.933 kb on + strand, within yadP at 161.933 kb on + strand, within yadP at 161.938 kb on + strand, within yadP at 161.945 kb on + strand, within yadP at 161.965 kb on + strand, within yadP at 162.008 kb on + strand at 162.030 kb on - strand at 162.038 kb on - strand at 162.038 kb on - strand at 162.068 kb on - strand at 162.068 kb on - strand at 162.071 kb on - strand at 162.091 kb on - strand at 162.091 kb on - strand at 162.100 kb on + strand at 162.129 kb on + strand at 162.129 kb on + strand at 162.137 kb on - strand at 162.148 kb on - strand at 162.171 kb on + strand at 162.219 kb on - strand at 162.421 kb on + strand, within hrpB at 162.432 kb on - strand, within hrpB at 162.432 kb on - strand, within hrpB at 162.433 kb on - strand, within hrpB at 162.434 kb on - strand, within hrpB at 162.434 kb on - strand, within hrpB at 162.451 kb on + strand, within hrpB at 162.459 kb on - strand, within hrpB at 162.459 kb on - strand, within hrpB at 162.584 kb on - strand, within hrpB at 162.584 kb on - strand, within hrpB at 162.584 kb on - strand, within hrpB at 162.636 kb on - strand, within hrpB at 162.674 kb on + strand, within hrpB at 162.707 kb on + strand, within hrpB at 162.707 kb on + strand, within hrpB at 162.820 kb on - strand, within hrpB at 162.825 kb on - strand, within hrpB at 162.828 kb on + strand, within hrpB at 162.837 kb on + strand, within hrpB at 162.837 kb on + strand, within hrpB at 162.839 kb on + strand, within hrpB at 162.852 kb on - strand, within hrpB at 162.973 kb on - strand, within hrpB at 162.983 kb on - strand, within hrpB at 162.983 kb on - strand, within hrpB at 163.088 kb on + strand, within hrpB at 163.088 kb on + strand, within hrpB at 163.288 kb on - strand, within hrpB at 163.302 kb on - strand, within hrpB at 163.326 kb on - strand, within hrpB at 163.326 kb on - strand, within hrpB at 163.349 kb on - strand, within hrpB at 163.401 kb on - strand, within hrpB at 163.437 kb on - strand, within hrpB at 163.437 kb on - strand, within hrpB at 163.447 kb on - strand, within hrpB at 163.459 kb on - strand, within hrpB at 163.507 kb on + strand, within hrpB at 163.776 kb on - strand, within hrpB at 163.782 kb on + strand, within hrpB at 163.811 kb on + strand, within hrpB at 163.811 kb on - strand, within hrpB at 163.811 kb on - strand, within hrpB at 163.819 kb on - strand, within hrpB at 163.819 kb on - strand, within hrpB at 163.834 kb on - strand, within hrpB at 163.904 kb on + strand, within hrpB at 163.904 kb on + strand, within hrpB at 163.938 kb on + strand, within hrpB at 163.938 kb on + strand, within hrpB at 164.040 kb on + strand, within hrpB at 164.040 kb on + strand, within hrpB at 164.114 kb on - strand, within hrpB at 164.115 kb on + strand, within hrpB at 164.174 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.227 kb on - strand, within hrpB at 164.237 kb on - strand, within hrpB at 164.237 kb on - strand, within hrpB at 164.364 kb on - strand at 164.366 kb on + strand at 164.374 kb on - strand at 164.374 kb on - strand at 164.422 kb on - strand at 164.496 kb on - strand at 164.525 kb on + strand at 164.525 kb on + strand at 164.553 kb on + strand at 164.568 kb on - strand at 164.568 kb on - strand at 164.604 kb on + strand at 164.606 kb on - strand at 164.612 kb on - strand at 164.612 kb on - strand at 164.625 kb on - strand at 164.649 kb on - strand at 164.712 kb on + strand at 164.728 kb on - strand at 164.737 kb on + strand at 164.788 kb on + strand at 164.844 kb on + strand at 164.844 kb on + strand at 164.846 kb on + strand at 165.082 kb on - strand, within mrcB at 165.089 kb on - strand, within mrcB at 165.127 kb on + strand, within mrcB at 165.181 kb on + strand, within mrcB at 165.228 kb on - strand, within mrcB at 165.305 kb on + strand, within mrcB at 165.311 kb on + strand, within mrcB at 165.363 kb on - strand, within mrcB at 165.419 kb on + strand, within mrcB at 165.468 kb on - strand, within mrcB at 165.475 kb on + strand, within mrcB at 165.513 kb on + strand, within mrcB at 165.519 kb on - strand, within mrcB at 165.521 kb on - strand, within mrcB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.000003125 mM remove 161,141 - sfsA b0146 0.51 +0.8 161,146 - sfsA b0146 0.52 +0.3 161,202 + sfsA b0146 0.60 +2.8 161,202 + sfsA b0146 0.60 +3.7 161,215 - sfsA b0146 0.61 +0.3 161,351 + sfsA b0146 0.81 +5.2 161,375 + sfsA b0146 0.84 +5.0 161,375 + sfsA b0146 0.84 +7.0 161,385 + sfsA b0146 0.86 +5.0 161,385 + sfsA b0146 0.86 +5.2 161,393 - sfsA b0146 0.87 -0.3 161,393 - sfsA b0146 0.87 +1.3 161,393 - sfsA b0146 0.87 +0.5 161,393 - sfsA b0146 0.87 +0.0 161,398 - sfsA b0146 0.87 +0.8 161,418 - -0.3 161,418 - +0.4 161,437 + +5.3 161,463 + +4.5 161,463 + +4.6 161,463 + +5.0 161,463 + +4.8 161,471 - +0.0 161,471 - +1.1 161,471 - -0.4 161,471 - -0.0 161,529 + +5.0 161,529 + +5.2 161,532 + +5.5 161,545 - -1.2 161,564 + yadP b0147 0.12 +5.0 161,600 + yadP b0147 0.19 +4.6 161,652 + yadP b0147 0.28 +5.9 161,659 - yadP b0147 0.30 +1.1 161,714 + yadP b0147 0.40 +5.6 161,758 + yadP b0147 0.48 +4.5 161,758 + yadP b0147 0.48 +6.3 161,758 + yadP b0147 0.48 +5.6 161,780 + yadP b0147 0.53 +6.0 161,780 + yadP b0147 0.53 +5.8 161,791 + yadP b0147 0.55 +6.3 161,804 - yadP b0147 0.57 +0.7 161,854 + yadP b0147 0.66 +6.0 161,933 + yadP b0147 0.81 +7.5 161,933 + yadP b0147 0.81 +6.9 161,938 + yadP b0147 0.82 +6.5 161,945 + yadP b0147 0.84 +7.2 161,965 + yadP b0147 0.87 +7.4 162,008 + +6.1 162,030 - -0.1 162,038 - +0.4 162,038 - +0.4 162,068 - +1.5 162,068 - -0.8 162,071 - -2.7 162,091 - -0.4 162,091 - -1.8 162,100 + +3.9 162,129 + +6.0 162,129 + +6.6 162,137 - +0.5 162,148 - +0.9 162,171 + +7.8 162,219 - -0.4 162,421 + hrpB b0148 0.13 +6.3 162,432 - hrpB b0148 0.13 +0.3 162,432 - hrpB b0148 0.13 -0.5 162,433 - hrpB b0148 0.13 +0.4 162,434 - hrpB b0148 0.14 +0.1 162,434 - hrpB b0148 0.14 -0.4 162,451 + hrpB b0148 0.14 +7.4 162,459 - hrpB b0148 0.15 +0.7 162,459 - hrpB b0148 0.15 +2.9 162,584 - hrpB b0148 0.20 -0.4 162,584 - hrpB b0148 0.20 -0.6 162,584 - hrpB b0148 0.20 +1.1 162,636 - hrpB b0148 0.22 +0.4 162,674 + hrpB b0148 0.23 +7.2 162,707 + hrpB b0148 0.25 +7.7 162,707 + hrpB b0148 0.25 +6.3 162,820 - hrpB b0148 0.29 -1.5 162,825 - hrpB b0148 0.30 -0.3 162,828 + hrpB b0148 0.30 +6.9 162,837 + hrpB b0148 0.30 +6.3 162,837 + hrpB b0148 0.30 +6.3 162,839 + hrpB b0148 0.30 +7.7 162,852 - hrpB b0148 0.31 -0.4 162,973 - hrpB b0148 0.36 +0.7 162,983 - hrpB b0148 0.36 -0.5 162,983 - hrpB b0148 0.36 -1.0 163,088 + hrpB b0148 0.40 +6.8 163,088 + hrpB b0148 0.40 +6.4 163,288 - hrpB b0148 0.49 +0.7 163,302 - hrpB b0148 0.49 +0.0 163,326 - hrpB b0148 0.50 -0.5 163,326 - hrpB b0148 0.50 -0.1 163,349 - hrpB b0148 0.51 +0.5 163,401 - hrpB b0148 0.53 -1.1 163,437 - hrpB b0148 0.55 +1.0 163,437 - hrpB b0148 0.55 +0.3 163,447 - hrpB b0148 0.55 +0.8 163,459 - hrpB b0148 0.56 -0.3 163,507 + hrpB b0148 0.58 +6.6 163,776 - hrpB b0148 0.69 +0.2 163,782 + hrpB b0148 0.69 +7.0 163,811 + hrpB b0148 0.70 +7.1 163,811 - hrpB b0148 0.70 +0.5 163,811 - hrpB b0148 0.70 -0.5 163,819 - hrpB b0148 0.71 +1.1 163,819 - hrpB b0148 0.71 +1.1 163,834 - hrpB b0148 0.71 +0.3 163,904 + hrpB b0148 0.74 +5.7 163,904 + hrpB b0148 0.74 +4.8 163,938 + hrpB b0148 0.75 +6.1 163,938 + hrpB b0148 0.75 +4.7 164,040 + hrpB b0148 0.80 +7.1 164,040 + hrpB b0148 0.80 +7.0 164,114 - hrpB b0148 0.83 -0.5 164,115 + hrpB b0148 0.83 +7.0 164,174 + hrpB b0148 0.85 +7.0 164,219 + hrpB b0148 0.87 +6.8 164,219 + hrpB b0148 0.87 +6.9 164,219 + hrpB b0148 0.87 +6.6 164,219 + hrpB b0148 0.87 +6.5 164,227 - hrpB b0148 0.87 +0.4 164,237 - hrpB b0148 0.88 -0.3 164,237 - hrpB b0148 0.88 -0.5 164,364 - -0.1 164,366 + +6.3 164,374 - -0.7 164,374 - -1.1 164,422 - -0.8 164,496 - -1.1 164,525 + +6.1 164,525 + +6.0 164,553 + +6.7 164,568 - -1.0 164,568 - -0.4 164,604 + +5.6 164,606 - +1.5 164,612 - +0.7 164,612 - -0.2 164,625 - -0.5 164,649 - -0.6 164,712 + +7.4 164,728 - +4.3 164,737 + +1.1 164,788 + +3.9 164,844 + -1.1 164,844 + -2.7 164,846 + +2.5 165,082 - mrcB b0149 0.14 -1.7 165,089 - mrcB b0149 0.14 -2.3 165,127 + mrcB b0149 0.16 -2.2 165,181 + mrcB b0149 0.18 -2.7 165,228 - mrcB b0149 0.20 -2.3 165,305 + mrcB b0149 0.23 -1.7 165,311 + mrcB b0149 0.23 -1.5 165,363 - mrcB b0149 0.25 -1.1 165,419 + mrcB b0149 0.27 -1.8 165,468 - mrcB b0149 0.29 -2.0 165,475 + mrcB b0149 0.29 -1.3 165,513 + mrcB b0149 0.31 -0.5 165,519 - mrcB b0149 0.31 -3.0 165,521 - mrcB b0149 0.31 -2.4
Or see this region's nucleotide sequence