Strain Fitness in Escherichia coli BW25113 around b1559

Experiment: LB Anaerobic with Mecillinam 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcspB and cspF are separated by 300 nucleotidescspF and b1559 are separated by 421 nucleotidesb1559 and ydfU are separated by 13 nucleotides b1557: cspB - Qin prophage; cold shock protein (NCBI), at 1,639,363 to 1,639,578 cspB b1558: cspF - Qin prophage; cold shock protein (NCBI), at 1,639,879 to 1,640,091 cspF b1559: b1559 - orf, hypothetical protein (VIMSS), at 1,640,513 to 1,641,265 b1559 b1560: ydfU - Qin prophage; predicted protein (RefSeq), at 1,641,279 to 1,642,328 ydfU Position (kb) 1640 1641 1642Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1639.632 kb on - strandat 1639.632 kb on - strandat 1639.733 kb on - strandat 1639.753 kb on - strandat 1639.797 kb on + strandat 1639.806 kb on - strandat 1639.834 kb on - strandat 1640.045 kb on + strand, within cspFat 1640.045 kb on + strand, within cspFat 1640.069 kb on - strand, within cspFat 1640.110 kb on + strandat 1640.118 kb on - strandat 1640.124 kb on + strandat 1640.128 kb on + strandat 1640.132 kb on - strandat 1640.132 kb on - strandat 1640.132 kb on - strandat 1640.203 kb on + strandat 1640.204 kb on - strandat 1640.204 kb on - strandat 1640.204 kb on - strandat 1640.265 kb on + strandat 1640.350 kb on + strandat 1640.365 kb on - strandat 1640.603 kb on - strand, within b1559at 1640.620 kb on - strand, within b1559at 1640.620 kb on - strand, within b1559at 1640.635 kb on - strand, within b1559at 1640.635 kb on - strand, within b1559at 1640.771 kb on - strand, within b1559at 1640.876 kb on - strand, within b1559at 1640.876 kb on - strand, within b1559at 1640.884 kb on + strand, within b1559at 1640.884 kb on + strand, within b1559at 1640.967 kb on - strand, within b1559at 1641.185 kb on - strand, within b1559at 1641.196 kb on + strandat 1641.253 kb on + strandat 1641.585 kb on + strand, within ydfUat 1641.585 kb on + strand, within ydfUat 1641.779 kb on + strand, within ydfUat 1641.831 kb on - strand, within ydfUat 1641.934 kb on - strand, within ydfUat 1642.013 kb on - strand, within ydfUat 1642.253 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Mecillinam 0.0000078125 mM
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1,639,632 - -0.3
1,639,632 - +0.1
1,639,733 - +0.2
1,639,753 - +0.1
1,639,797 + +0.5
1,639,806 - -1.0
1,639,834 - +0.5
1,640,045 + cspF b1558 0.78 +1.4
1,640,045 + cspF b1558 0.78 -1.0
1,640,069 - cspF b1558 0.89 +0.5
1,640,110 + +0.0
1,640,118 - +1.5
1,640,124 + +1.1
1,640,128 + -2.0
1,640,132 - +2.9
1,640,132 - +0.7
1,640,132 - -0.3
1,640,203 + -0.5
1,640,204 - +0.5
1,640,204 - +0.5
1,640,204 - +0.5
1,640,265 + -1.0
1,640,350 + +0.5
1,640,365 - +0.4
1,640,603 - b1559 b1559 0.12 +0.7
1,640,620 - b1559 b1559 0.14 -0.1
1,640,620 - b1559 b1559 0.14 +0.1
1,640,635 - b1559 b1559 0.16 +0.1
1,640,635 - b1559 b1559 0.16 -0.5
1,640,771 - b1559 b1559 0.34 +1.1
1,640,876 - b1559 b1559 0.48 -0.7
1,640,876 - b1559 b1559 0.48 +1.9
1,640,884 + b1559 b1559 0.49 -0.0
1,640,884 + b1559 b1559 0.49 -0.0
1,640,967 - b1559 b1559 0.60 +0.1
1,641,185 - b1559 b1559 0.89 +0.1
1,641,196 + -1.3
1,641,253 + +0.5
1,641,585 + ydfU b1560 0.29 +0.2
1,641,585 + ydfU b1560 0.29 -0.8
1,641,779 + ydfU b1560 0.48 +0.1
1,641,831 - ydfU b1560 0.53 +0.6
1,641,934 - ydfU b1560 0.62 -0.6
1,642,013 - ydfU b1560 0.70 +0.1
1,642,253 - +0.6

Or see this region's nucleotide sequence