Strain Fitness in Escherichia coli BW25113 around b3967

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbtuB and murI overlap by 56 nucleotidesmurI and rrsB are separated by 373 nucleotides b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506 btuB b3967: murI - glutamate racemase, required for biosynthesis of D-glutamate and peptidoglycan (VIMSS), at 4,163,451 to 4,164,308 murI b3968: rrsB - 16S ribosomal RNA (NCBI), at 4,164,682 to 4,166,223 rrsB Position (kb) 4163 4164 4165Strain fitness (log2 ratio) -2 -1 0 1at 4162.514 kb on + strand, within btuBat 4162.524 kb on + strand, within btuBat 4162.532 kb on - strand, within btuBat 4162.537 kb on - strand, within btuBat 4162.541 kb on + strand, within btuBat 4162.541 kb on + strand, within btuBat 4162.591 kb on + strand, within btuBat 4162.596 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.603 kb on + strand, within btuBat 4162.658 kb on - strand, within btuBat 4162.661 kb on + strand, within btuBat 4162.669 kb on - strand, within btuBat 4162.669 kb on - strand, within btuBat 4162.670 kb on - strand, within btuBat 4162.701 kb on - strand, within btuBat 4162.701 kb on - strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.706 kb on + strand, within btuBat 4162.716 kb on + strand, within btuBat 4162.721 kb on + strand, within btuBat 4162.721 kb on + strand, within btuBat 4162.726 kb on + strand, within btuBat 4162.726 kb on + strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.734 kb on - strand, within btuBat 4162.741 kb on + strand, within btuBat 4162.741 kb on + strand, within btuBat 4162.776 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.785 kb on + strand, within btuBat 4162.822 kb on + strand, within btuBat 4162.822 kb on + strand, within btuBat 4162.830 kb on - strand, within btuBat 4162.830 kb on - strand, within btuBat 4162.866 kb on + strand, within btuBat 4162.885 kb on + strand, within btuBat 4162.918 kb on - strand, within btuBat 4162.924 kb on + strand, within btuBat 4162.924 kb on + strand, within btuBat 4162.985 kb on + strand, within btuBat 4162.985 kb on + strand, within btuBat 4163.011 kb on - strand, within btuBat 4163.109 kb on - strand, within btuBat 4163.141 kb on + strand, within btuBat 4163.141 kb on + strand, within btuBat 4163.146 kb on + strand, within btuBat 4163.148 kb on + strand, within btuBat 4163.156 kb on - strand, within btuBat 4163.168 kb on - strand, within btuBat 4163.212 kb on - strand, within btuBat 4163.212 kb on - strand, within btuBat 4163.214 kb on + strand, within btuBat 4163.214 kb on + strand, within btuBat 4163.269 kb on + strand, within btuBat 4163.269 kb on + strand, within btuBat 4163.272 kb on - strand, within btuBat 4163.272 kb on - strand, within btuBat 4163.317 kb on + strand, within btuBat 4163.357 kb on - strandat 4163.357 kb on - strandat 4163.357 kb on - strandat 4163.388 kb on + strandat 4163.388 kb on + strandat 4163.447 kb on + strandat 4163.447 kb on + strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.455 kb on - strandat 4163.505 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM
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4,162,514 + btuB b3966 0.46 -0.9
4,162,524 + btuB b3966 0.47 -0.4
4,162,532 - btuB b3966 0.47 -0.2
4,162,537 - btuB b3966 0.47 +0.2
4,162,541 + btuB b3966 0.48 -0.3
4,162,541 + btuB b3966 0.48 +0.7
4,162,591 + btuB b3966 0.50 +0.6
4,162,596 + btuB b3966 0.51 -0.4
4,162,603 + btuB b3966 0.51 -0.9
4,162,603 + btuB b3966 0.51 -1.7
4,162,603 + btuB b3966 0.51 +0.2
4,162,603 + btuB b3966 0.51 +0.3
4,162,658 - btuB b3966 0.54 -0.5
4,162,661 + btuB b3966 0.54 -1.3
4,162,669 - btuB b3966 0.55 -0.3
4,162,669 - btuB b3966 0.55 -0.1
4,162,670 - btuB b3966 0.55 +1.1
4,162,701 - btuB b3966 0.56 +0.7
4,162,701 - btuB b3966 0.56 -0.9
4,162,706 + btuB b3966 0.57 -0.5
4,162,706 + btuB b3966 0.57 -0.3
4,162,706 + btuB b3966 0.57 -0.7
4,162,716 + btuB b3966 0.57 +0.2
4,162,721 + btuB b3966 0.57 -0.5
4,162,721 + btuB b3966 0.57 -0.4
4,162,726 + btuB b3966 0.58 -0.4
4,162,726 + btuB b3966 0.58 +0.3
4,162,734 - btuB b3966 0.58 +0.4
4,162,734 - btuB b3966 0.58 +0.1
4,162,734 - btuB b3966 0.58 -0.3
4,162,741 + btuB b3966 0.58 +1.1
4,162,741 + btuB b3966 0.58 -0.5
4,162,776 + btuB b3966 0.60 -0.6
4,162,785 + btuB b3966 0.61 +1.1
4,162,785 + btuB b3966 0.61 +0.3
4,162,785 + btuB b3966 0.61 -0.6
4,162,822 + btuB b3966 0.63 -0.7
4,162,822 + btuB b3966 0.63 +0.3
4,162,830 - btuB b3966 0.63 -0.3
4,162,830 - btuB b3966 0.63 -0.5
4,162,866 + btuB b3966 0.65 +0.1
4,162,885 + btuB b3966 0.66 -0.4
4,162,918 - btuB b3966 0.68 -0.4
4,162,924 + btuB b3966 0.68 +0.3
4,162,924 + btuB b3966 0.68 -1.8
4,162,985 + btuB b3966 0.72 +0.1
4,162,985 + btuB b3966 0.72 -0.7
4,163,011 - btuB b3966 0.73 +0.8
4,163,109 - btuB b3966 0.78 -1.1
4,163,141 + btuB b3966 0.80 +0.4
4,163,141 + btuB b3966 0.80 +0.1
4,163,146 + btuB b3966 0.80 +0.6
4,163,148 + btuB b3966 0.81 +0.5
4,163,156 - btuB b3966 0.81 +0.2
4,163,168 - btuB b3966 0.82 -0.9
4,163,212 - btuB b3966 0.84 +0.2
4,163,212 - btuB b3966 0.84 -0.5
4,163,214 + btuB b3966 0.84 -0.1
4,163,214 + btuB b3966 0.84 -1.1
4,163,269 + btuB b3966 0.87 -1.5
4,163,269 + btuB b3966 0.87 -2.2
4,163,272 - btuB b3966 0.87 +1.5
4,163,272 - btuB b3966 0.87 -0.1
4,163,317 + btuB b3966 0.90 -0.6
4,163,357 - +0.9
4,163,357 - -0.5
4,163,357 - +0.6
4,163,388 + +0.5
4,163,388 + -0.1
4,163,447 + +1.1
4,163,447 + -0.3
4,163,455 - -0.4
4,163,455 - +0.5
4,163,455 - +0.6
4,163,455 - -1.7
4,163,455 - +0.6
4,163,505 + -0.9

Or see this region's nucleotide sequence