Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldcA and mltE are separated by 99 nucleotides mltE and ycgR are separated by 1 nucleotides ycgR and ymgE are separated by 200 nucleotides
b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303
ldcA
b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014
mltE
b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750
ycgR
b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205
ymgE
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1241.424 kb on + strand at 1241.425 kb on - strand at 1241.433 kb on + strand at 1241.501 kb on + strand, within ldcA at 1241.572 kb on - strand, within ldcA at 1241.581 kb on + strand, within ldcA at 1241.747 kb on + strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.999 kb on - strand, within ldcA at 1242.033 kb on + strand, within ldcA at 1242.208 kb on - strand, within ldcA at 1242.237 kb on - strand at 1242.284 kb on + strand at 1242.305 kb on - strand at 1242.337 kb on + strand at 1242.355 kb on - strand at 1242.365 kb on - strand at 1242.365 kb on - strand at 1242.465 kb on + strand, within mltE at 1242.655 kb on + strand, within mltE at 1243.159 kb on + strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.521 kb on + strand, within ycgR at 1243.589 kb on + strand, within ycgR at 1243.637 kb on - strand, within ycgR at 1243.688 kb on + strand at 1243.722 kb on - strand at 1243.732 kb on + strand at 1243.798 kb on + strand at 1243.864 kb on + strand at 1243.875 kb on + strand at 1243.890 kb on + strand at 1243.893 kb on - strand at 1243.899 kb on + strand at 1243.904 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM remove 1,241,424 + -4.2 1,241,425 - -2.3 1,241,433 + -2.8 1,241,501 + ldcA b1192 0.12 -3.7 1,241,572 - ldcA b1192 0.20 -2.6 1,241,581 + ldcA b1192 0.21 -2.0 1,241,747 + ldcA b1192 0.39 -1.5 1,241,936 - ldcA b1192 0.60 -3.7 1,241,936 - ldcA b1192 0.60 -3.8 1,241,961 - ldcA b1192 0.63 -2.9 1,241,961 - ldcA b1192 0.63 -1.6 1,241,999 - ldcA b1192 0.67 -2.3 1,242,033 + ldcA b1192 0.70 -3.2 1,242,208 - ldcA b1192 0.90 -2.3 1,242,237 - -2.0 1,242,284 + -0.5 1,242,305 - -2.4 1,242,337 + -0.5 1,242,355 - +0.6 1,242,365 - -0.2 1,242,365 - +0.2 1,242,465 + mltE b1193 0.10 -0.1 1,242,655 + mltE b1193 0.41 -0.6 1,243,159 + ycgR b1194 0.19 -1.7 1,243,291 - ycgR b1194 0.37 -1.2 1,243,291 - ycgR b1194 0.37 -0.2 1,243,521 + ycgR b1194 0.69 +0.0 1,243,589 + ycgR b1194 0.78 -0.1 1,243,637 - ycgR b1194 0.84 -1.0 1,243,688 + +0.2 1,243,722 - +0.5 1,243,732 + +0.5 1,243,798 + -0.3 1,243,864 + -0.6 1,243,875 + +0.5 1,243,890 + -2.2 1,243,893 - +0.5 1,243,899 + +0.1 1,243,904 + +2.0
Or see this region's nucleotide sequence