Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaB and ygdG are separated by 111 nucleotides ygdG and fucO are separated by 54 nucleotides fucO and fucA are separated by 27 nucleotides
b2797: sdaB - L-serine deaminase II (NCBI), at 2,927,598 to 2,928,965
sdaB
b2798: ygdG - Ssb-binding protein, misidentified as ExoIX (RefSeq), at 2,929,077 to 2,929,832
ygdG
b2799: fucO - L-1,2-propanediol oxidoreductase (NCBI), at 2,929,887 to 2,931,035
fucO
b2800: fucA - L-fuculose phosphate aldolase (NCBI), at 2,931,063 to 2,931,710
fucA
Position (kb)
2929
2930
2931
2932 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2928.887 kb on - strand at 2928.887 kb on - strand at 2928.919 kb on - strand at 2928.919 kb on - strand at 2928.956 kb on - strand at 2928.956 kb on - strand at 2929.025 kb on - strand at 2929.158 kb on - strand, within ygdG at 2929.189 kb on - strand, within ygdG at 2929.335 kb on + strand, within ygdG at 2929.353 kb on + strand, within ygdG at 2929.391 kb on - strand, within ygdG at 2929.652 kb on + strand, within ygdG at 2929.680 kb on - strand, within ygdG at 2929.683 kb on + strand, within ygdG at 2929.683 kb on + strand, within ygdG at 2929.891 kb on - strand at 2929.915 kb on - strand at 2929.970 kb on - strand at 2929.972 kb on + strand at 2929.974 kb on - strand at 2929.980 kb on - strand at 2929.980 kb on - strand at 2929.996 kb on + strand at 2929.996 kb on + strand at 2930.006 kb on + strand, within fucO at 2930.006 kb on + strand, within fucO at 2930.137 kb on + strand, within fucO at 2930.161 kb on + strand, within fucO at 2930.161 kb on + strand, within fucO at 2930.189 kb on - strand, within fucO at 2930.198 kb on + strand, within fucO at 2930.215 kb on - strand, within fucO at 2930.248 kb on - strand, within fucO at 2930.280 kb on + strand, within fucO at 2930.288 kb on - strand, within fucO at 2930.298 kb on - strand, within fucO at 2930.298 kb on - strand, within fucO at 2930.362 kb on + strand, within fucO at 2930.367 kb on + strand, within fucO at 2930.406 kb on - strand, within fucO at 2930.406 kb on - strand, within fucO at 2930.417 kb on + strand at 2930.427 kb on + strand, within fucO at 2930.427 kb on + strand, within fucO at 2930.622 kb on - strand, within fucO at 2930.660 kb on + strand, within fucO at 2930.676 kb on + strand, within fucO at 2930.677 kb on - strand, within fucO at 2930.806 kb on - strand, within fucO at 2930.806 kb on - strand, within fucO at 2930.832 kb on - strand, within fucO at 2930.856 kb on + strand, within fucO at 2930.872 kb on - strand, within fucO at 2930.877 kb on - strand, within fucO at 2930.877 kb on - strand, within fucO at 2930.892 kb on + strand, within fucO at 2931.131 kb on + strand, within fucA at 2931.172 kb on + strand, within fucA at 2931.349 kb on - strand, within fucA at 2931.349 kb on - strand, within fucA at 2931.381 kb on + strand, within fucA at 2931.435 kb on - strand, within fucA at 2931.489 kb on - strand, within fucA at 2931.528 kb on + strand, within fucA at 2931.528 kb on + strand, within fucA at 2931.549 kb on + strand, within fucA at 2931.596 kb on + strand, within fucA at 2931.602 kb on - strand, within fucA at 2931.602 kb on - strand, within fucA at 2931.606 kb on - strand, within fucA at 2931.606 kb on - strand, within fucA at 2931.606 kb on - strand, within fucA at 2931.611 kb on + strand, within fucA at 2931.611 kb on + strand, within fucA at 2931.611 kb on + strand, within fucA at 2931.613 kb on + strand, within fucA at 2931.613 kb on + strand, within fucA at 2931.621 kb on + strand, within fucA at 2931.637 kb on + strand, within fucA at 2931.644 kb on - strand, within fucA at 2931.644 kb on - strand, within fucA at 2931.645 kb on - strand, within fucA at 2931.650 kb on - strand at 2931.650 kb on - strand at 2931.655 kb on - strand at 2931.673 kb on + strand at 2931.694 kb on + strand at 2931.694 kb on + strand at 2931.707 kb on + strand at 2931.711 kb on + strand at 2931.711 kb on + strand at 2931.711 kb on - strand at 2931.735 kb on - strand at 2931.735 kb on - strand at 2931.735 kb on - strand at 2931.748 kb on - strand at 2931.753 kb on + strand at 2931.762 kb on + strand at 2931.762 kb on + strand at 2931.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM remove 2,928,887 - +2.5 2,928,887 - -0.6 2,928,919 - -1.6 2,928,919 - -0.5 2,928,956 - -0.2 2,928,956 - +0.9 2,929,025 - +0.5 2,929,158 - ygdG b2798 0.11 -0.8 2,929,189 - ygdG b2798 0.15 +0.0 2,929,335 + ygdG b2798 0.34 -2.5 2,929,353 + ygdG b2798 0.37 -0.1 2,929,391 - ygdG b2798 0.42 +0.4 2,929,652 + ygdG b2798 0.76 -0.4 2,929,680 - ygdG b2798 0.80 +0.7 2,929,683 + ygdG b2798 0.80 -0.5 2,929,683 + ygdG b2798 0.80 +1.5 2,929,891 - -0.2 2,929,915 - -0.8 2,929,970 - +0.8 2,929,972 + -1.6 2,929,974 - -0.8 2,929,980 - -0.1 2,929,980 - -0.1 2,929,996 + -1.0 2,929,996 + +1.5 2,930,006 + fucO b2799 0.10 +0.7 2,930,006 + fucO b2799 0.10 +1.9 2,930,137 + fucO b2799 0.22 -0.0 2,930,161 + fucO b2799 0.24 -0.8 2,930,161 + fucO b2799 0.24 +0.5 2,930,189 - fucO b2799 0.26 -1.1 2,930,198 + fucO b2799 0.27 +0.0 2,930,215 - fucO b2799 0.29 -2.2 2,930,248 - fucO b2799 0.31 -0.6 2,930,280 + fucO b2799 0.34 +0.3 2,930,288 - fucO b2799 0.35 +0.8 2,930,298 - fucO b2799 0.36 -1.6 2,930,298 - fucO b2799 0.36 -1.1 2,930,362 + fucO b2799 0.41 -0.1 2,930,367 + fucO b2799 0.42 -0.9 2,930,406 - fucO b2799 0.45 +0.7 2,930,406 - fucO b2799 0.45 -1.5 2,930,417 + +0.5 2,930,427 + fucO b2799 0.47 -0.3 2,930,427 + fucO b2799 0.47 -1.5 2,930,622 - fucO b2799 0.64 +1.5 2,930,660 + fucO b2799 0.67 -0.7 2,930,676 + fucO b2799 0.69 -0.1 2,930,677 - fucO b2799 0.69 -0.7 2,930,806 - fucO b2799 0.80 -1.5 2,930,806 - fucO b2799 0.80 +0.5 2,930,832 - fucO b2799 0.82 +0.7 2,930,856 + fucO b2799 0.84 -0.5 2,930,872 - fucO b2799 0.86 -1.3 2,930,877 - fucO b2799 0.86 -1.1 2,930,877 - fucO b2799 0.86 -1.4 2,930,892 + fucO b2799 0.87 -0.5 2,931,131 + fucA b2800 0.10 +0.4 2,931,172 + fucA b2800 0.17 -0.5 2,931,349 - fucA b2800 0.44 -1.2 2,931,349 - fucA b2800 0.44 -0.9 2,931,381 + fucA b2800 0.49 +0.1 2,931,435 - fucA b2800 0.57 -1.7 2,931,489 - fucA b2800 0.66 +0.5 2,931,528 + fucA b2800 0.72 +2.1 2,931,528 + fucA b2800 0.72 -0.4 2,931,549 + fucA b2800 0.75 -1.1 2,931,596 + fucA b2800 0.82 +1.5 2,931,602 - fucA b2800 0.83 -0.2 2,931,602 - fucA b2800 0.83 +1.1 2,931,606 - fucA b2800 0.84 -0.2 2,931,606 - fucA b2800 0.84 +0.9 2,931,606 - fucA b2800 0.84 +0.8 2,931,611 + fucA b2800 0.85 +0.0 2,931,611 + fucA b2800 0.85 +0.5 2,931,611 + fucA b2800 0.85 +0.9 2,931,613 + fucA b2800 0.85 +0.5 2,931,613 + fucA b2800 0.85 -0.1 2,931,621 + fucA b2800 0.86 +0.4 2,931,637 + fucA b2800 0.89 +0.3 2,931,644 - fucA b2800 0.90 +1.5 2,931,644 - fucA b2800 0.90 -1.3 2,931,645 - fucA b2800 0.90 +1.5 2,931,650 - +1.5 2,931,650 - -0.1 2,931,655 - +2.5 2,931,673 + +0.5 2,931,694 + +0.5 2,931,694 + -0.1 2,931,707 + -0.1 2,931,711 + +1.5 2,931,711 + +2.5 2,931,711 - +1.5 2,931,735 - -0.6 2,931,735 - -1.5 2,931,735 - +1.5 2,931,748 - +0.9 2,931,753 + -0.8 2,931,762 + -0.4 2,931,762 + -0.5 2,931,764 + +0.5
Or see this region's nucleotide sequence