Strain Fitness in Escherichia coli BW25113 around b2701

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecA and ygaD are separated by 79 nucleotidesygaD and mltB are separated by 144 nucleotidesmltB and srlA are separated by 255 nucleotidessrlA and srlE overlap by 4 nucleotides b2699: recA - recombinase A (NCBI), at 2,820,730 to 2,821,791 recA b2700: ygaD - competence damage-inducible protein A (NCBI), at 2,821,871 to 2,822,368 ygaD b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598 mltB b2702: srlA - glucitol/sorbitol-specific enzyme IIC component of PTS (NCBI), at 2,823,854 to 2,824,417 srlA b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373 srlE Position (kb) 2822 2823 2824Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2821.522 kb on + strand, within recAat 2821.638 kb on + strand, within recAat 2821.701 kb on - strandat 2821.810 kb on - strandat 2821.810 kb on - strandat 2822.013 kb on + strand, within ygaDat 2822.231 kb on - strand, within ygaDat 2822.232 kb on + strand, within ygaDat 2822.333 kb on - strandat 2822.358 kb on + strandat 2822.437 kb on - strandat 2822.488 kb on + strandat 2822.520 kb on + strandat 2822.520 kb on + strandat 2822.545 kb on - strandat 2822.598 kb on - strandat 2822.641 kb on - strand, within mltBat 2822.668 kb on + strand, within mltBat 2822.764 kb on - strand, within mltBat 2822.765 kb on - strand, within mltBat 2822.827 kb on + strand, within mltBat 2822.840 kb on - strand, within mltBat 2822.880 kb on - strand, within mltBat 2822.996 kb on + strand, within mltBat 2823.026 kb on - strand, within mltBat 2823.150 kb on + strand, within mltBat 2823.162 kb on + strand, within mltBat 2823.183 kb on + strand, within mltBat 2823.256 kb on + strand, within mltBat 2823.279 kb on - strand, within mltBat 2823.486 kb on - strand, within mltBat 2823.486 kb on - strand, within mltBat 2823.519 kb on - strandat 2823.519 kb on - strandat 2823.633 kb on + strandat 2823.673 kb on - strandat 2823.673 kb on - strandat 2823.689 kb on - strandat 2823.807 kb on - strandat 2823.849 kb on + strandat 2823.872 kb on - strandat 2823.876 kb on + strandat 2823.939 kb on + strand, within srlAat 2823.939 kb on + strand, within srlAat 2823.970 kb on - strand, within srlAat 2823.976 kb on - strand, within srlAat 2824.104 kb on + strand, within srlAat 2824.104 kb on + strand, within srlAat 2824.117 kb on - strand, within srlAat 2824.196 kb on + strand, within srlAat 2824.229 kb on - strand, within srlAat 2824.229 kb on - strand, within srlAat 2824.287 kb on + strand, within srlAat 2824.287 kb on + strand, within srlAat 2824.300 kb on - strand, within srlAat 2824.412 kb on - strandat 2824.412 kb on - strandat 2824.460 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
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2,821,522 + recA b2699 0.75 +3.5
2,821,638 + recA b2699 0.85 +1.5
2,821,701 - +0.5
2,821,810 - -0.7
2,821,810 - +0.3
2,822,013 + ygaD b2700 0.29 -0.9
2,822,231 - ygaD b2700 0.72 -0.2
2,822,232 + ygaD b2700 0.72 -0.5
2,822,333 - -0.7
2,822,358 + -1.7
2,822,437 - -0.2
2,822,488 + +1.0
2,822,520 + +0.3
2,822,520 + +0.1
2,822,545 - +0.3
2,822,598 - +0.0
2,822,641 - mltB b2701 0.12 -0.5
2,822,668 + mltB b2701 0.14 -0.7
2,822,764 - mltB b2701 0.23 -0.6
2,822,765 - mltB b2701 0.23 -0.4
2,822,827 + mltB b2701 0.29 -0.1
2,822,840 - mltB b2701 0.30 -0.2
2,822,880 - mltB b2701 0.34 +0.3
2,822,996 + mltB b2701 0.44 -0.3
2,823,026 - mltB b2701 0.47 -2.6
2,823,150 + mltB b2701 0.59 +1.5
2,823,162 + mltB b2701 0.60 -0.1
2,823,183 + mltB b2701 0.62 +0.8
2,823,256 + mltB b2701 0.68 -0.1
2,823,279 - mltB b2701 0.71 +0.7
2,823,486 - mltB b2701 0.90 -0.1
2,823,486 - mltB b2701 0.90 -0.5
2,823,519 - -1.0
2,823,519 - +0.8
2,823,633 + +0.2
2,823,673 - -1.4
2,823,673 - -1.2
2,823,689 - +0.7
2,823,807 - +0.2
2,823,849 + +0.6
2,823,872 - -1.2
2,823,876 + +0.5
2,823,939 + srlA b2702 0.15 +0.5
2,823,939 + srlA b2702 0.15 +0.4
2,823,970 - srlA b2702 0.21 -0.4
2,823,976 - srlA b2702 0.22 -0.5
2,824,104 + srlA b2702 0.44 -0.9
2,824,104 + srlA b2702 0.44 -0.2
2,824,117 - srlA b2702 0.47 +0.3
2,824,196 + srlA b2702 0.61 -1.4
2,824,229 - srlA b2702 0.66 +0.2
2,824,229 - srlA b2702 0.66 +0.8
2,824,287 + srlA b2702 0.77 +1.5
2,824,287 + srlA b2702 0.77 -0.1
2,824,300 - srlA b2702 0.79 +0.5
2,824,412 - -0.1
2,824,412 - -1.3
2,824,460 + -0.9

Or see this region's nucleotide sequence