Strain Fitness in Escherichia coli BW25113 around b1634

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnth and tppB are separated by 610 nucleotidestppB and gst are separated by 105 nucleotidesgst and pdxY are separated by 43 nucleotides b1633: nth - DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI), at 1,709,547 to 1,710,182 nth b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295 tppB b1635: gst - glutathionine S-transferase (NCBI), at 1,712,401 to 1,713,006 gst b1636: pdxY - pyridoxine kinase (NCBI), at 1,713,050 to 1,713,913 pdxY Position (kb) 1710 1711 1712 1713Strain fitness (log2 ratio) -2 -1 0 1 2at 1709.843 kb on + strand, within nthat 1709.859 kb on + strand, within nthat 1709.859 kb on + strand, within nthat 1709.876 kb on + strand, within nthat 1709.876 kb on + strand, within nthat 1710.159 kb on + strandat 1710.274 kb on - strandat 1710.551 kb on - strandat 1710.837 kb on - strandat 1710.838 kb on - strandat 1710.870 kb on - strandat 1711.174 kb on - strand, within tppBat 1711.239 kb on - strand, within tppBat 1711.254 kb on + strand, within tppBat 1711.352 kb on + strand, within tppBat 1711.360 kb on - strand, within tppBat 1711.360 kb on - strand, within tppBat 1711.360 kb on - strand, within tppBat 1711.402 kb on + strand, within tppBat 1711.402 kb on + strand, within tppBat 1711.536 kb on + strand, within tppBat 1711.536 kb on + strand, within tppBat 1711.582 kb on - strand, within tppBat 1711.803 kb on - strand, within tppBat 1711.932 kb on - strand, within tppBat 1711.978 kb on - strand, within tppBat 1712.070 kb on - strand, within tppBat 1712.104 kb on - strand, within tppBat 1712.104 kb on - strand, within tppBat 1712.109 kb on + strand, within tppBat 1712.543 kb on - strand, within gstat 1712.623 kb on - strand, within gstat 1712.715 kb on + strand, within gstat 1713.214 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxY

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
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1,709,843 + nth b1633 0.47 -1.3
1,709,859 + nth b1633 0.49 +0.7
1,709,859 + nth b1633 0.49 +0.7
1,709,876 + nth b1633 0.52 -0.2
1,709,876 + nth b1633 0.52 -0.0
1,710,159 + -2.1
1,710,274 - -0.4
1,710,551 - -0.8
1,710,837 - +0.3
1,710,838 - +0.2
1,710,870 - +0.8
1,711,174 - tppB b1634 0.25 +0.2
1,711,239 - tppB b1634 0.30 -1.1
1,711,254 + tppB b1634 0.31 -1.6
1,711,352 + tppB b1634 0.37 -0.8
1,711,360 - tppB b1634 0.38 +1.2
1,711,360 - tppB b1634 0.38 +1.2
1,711,360 - tppB b1634 0.38 +0.2
1,711,402 + tppB b1634 0.41 -1.4
1,711,402 + tppB b1634 0.41 -0.4
1,711,536 + tppB b1634 0.49 +1.2
1,711,536 + tppB b1634 0.49 -1.1
1,711,582 - tppB b1634 0.52 -0.4
1,711,803 - tppB b1634 0.67 -0.9
1,711,932 - tppB b1634 0.76 -1.6
1,711,978 - tppB b1634 0.79 +1.2
1,712,070 - tppB b1634 0.85 +0.8
1,712,104 - tppB b1634 0.87 -1.4
1,712,104 - tppB b1634 0.87 -0.8
1,712,109 + tppB b1634 0.88 -1.4
1,712,543 - gst b1635 0.23 -1.0
1,712,623 - gst b1635 0.37 -1.5
1,712,715 + gst b1635 0.52 -0.0
1,713,214 + pdxY b1636 0.19 +0.4
1,713,223 + pdxY b1636 0.20 +2.0
1,713,223 + pdxY b1636 0.20 -0.8
1,713,223 + pdxY b1636 0.20 -1.1

Or see this region's nucleotide sequence