Strain Fitness in Escherichia coli BW25113 around b0087

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurF and mraY overlap by 7 nucleotidesmraY and murD are separated by 2 nucleotides b0086: murF - UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase (NCBI), at 94,650 to 96,008 murF b0087: mraY - phospho-N-acetylmuramoyl-pentapeptide-transferase (NCBI), at 96,002 to 97,084 mraY b0088: murD - UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI), at 97,087 to 98,403 murD Position (kb) 96 97 98Strain fitness (log2 ratio) -1 0 1at 95.980 kb on + strandat 95.980 kb on + strandat 95.983 kb on + strandat 95.983 kb on + strandat 95.985 kb on + strandat 97.078 kb on + strandat 97.329 kb on + strand, within murDat 97.380 kb on + strand, within murDat 97.787 kb on + strand, within murD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
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95,980 + -1.5
95,980 + -0.5
95,983 + -1.5
95,983 + -0.8
95,985 + +1.3
97,078 + +0.2
97,329 + murD b0088 0.18 -0.4
97,380 + murD b0088 0.22 -0.6
97,787 + murD b0088 0.53 +0.5

Or see this region's nucleotide sequence