Experiment: KB with Isosafrole 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2258 and Psyr_2259 overlap by 10 nucleotides Psyr_2259 and Psyr_2260 are separated by 9 nucleotides Psyr_2260 and Psyr_2261 are separated by 118 nucleotides
Psyr_2258: Psyr_2258 - Guanylate kinase/L-type calcium channel region, at 2,622,773 to 2,623,339
_2258
Psyr_2259: Psyr_2259 - Beta-lactamase-like protein, at 2,623,330 to 2,624,082
_2259
Psyr_2260: Psyr_2260 - Amidase, at 2,624,092 to 2,625,615
_2260
Psyr_2261: Psyr_2261 - Peptidase M20:Peptidase M20, at 2,625,734 to 2,626,885
_2261
Position (kb)
2624
2625
2626 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2623.378 kb on + strand at 2623.654 kb on + strand, within Psyr_2259 at 2623.762 kb on - strand, within Psyr_2259 at 2623.762 kb on - strand, within Psyr_2259 at 2624.048 kb on + strand at 2624.049 kb on - strand at 2624.049 kb on - strand at 2624.049 kb on - strand at 2624.049 kb on - strand at 2624.049 kb on - strand at 2624.049 kb on - strand at 2624.068 kb on - strand at 2624.135 kb on + strand at 2624.135 kb on + strand at 2624.378 kb on + strand, within Psyr_2260 at 2624.378 kb on + strand, within Psyr_2260 at 2624.379 kb on - strand, within Psyr_2260 at 2624.399 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.438 kb on + strand, within Psyr_2260 at 2624.438 kb on + strand, within Psyr_2260 at 2624.472 kb on + strand, within Psyr_2260 at 2624.473 kb on - strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2625.636 kb on + strand at 2625.637 kb on - strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.641 kb on - strand at 2625.641 kb on - strand at 2625.642 kb on + strand at 2625.643 kb on - strand at 2625.659 kb on + strand at 2625.660 kb on - strand at 2625.665 kb on + strand at 2625.670 kb on + strand at 2625.955 kb on - strand, within Psyr_2261 at 2625.955 kb on - strand, within Psyr_2261 at 2625.958 kb on + strand, within Psyr_2261 at 2625.958 kb on + strand, within Psyr_2261 at 2625.959 kb on - strand, within Psyr_2261 at 2625.959 kb on - strand, within Psyr_2261 at 2625.959 kb on - strand, within Psyr_2261 at 2626.076 kb on + strand, within Psyr_2261 at 2626.076 kb on + strand, within Psyr_2261 at 2626.156 kb on + strand, within Psyr_2261 at 2626.226 kb on + strand, within Psyr_2261 at 2626.226 kb on + strand, within Psyr_2261 at 2626.227 kb on - strand, within Psyr_2261
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Isosafrole 10 mM remove 2,623,378 + +2.0 2,623,654 + Psyr_2259 0.43 -1.3 2,623,762 - Psyr_2259 0.57 +0.7 2,623,762 - Psyr_2259 0.57 +0.8 2,624,048 + -0.5 2,624,049 - +0.0 2,624,049 - -0.3 2,624,049 - +3.0 2,624,049 - -0.4 2,624,049 - -0.4 2,624,049 - -0.3 2,624,068 - +1.3 2,624,135 + +1.5 2,624,135 + +0.3 2,624,378 + Psyr_2260 0.19 -0.7 2,624,378 + Psyr_2260 0.19 +0.4 2,624,379 - Psyr_2260 0.19 +2.3 2,624,399 + Psyr_2260 0.20 -0.1 2,624,402 + Psyr_2260 0.20 +0.7 2,624,402 + Psyr_2260 0.20 -0.3 2,624,402 + Psyr_2260 0.20 +0.3 2,624,403 - Psyr_2260 0.20 +0.3 2,624,438 + Psyr_2260 0.23 -0.6 2,624,438 + Psyr_2260 0.23 +0.2 2,624,472 + Psyr_2260 0.25 +0.2 2,624,473 - Psyr_2260 0.25 +0.2 2,624,495 + Psyr_2260 0.26 -0.3 2,624,495 + Psyr_2260 0.26 +0.2 2,624,881 - Psyr_2260 0.52 +1.0 2,624,881 - Psyr_2260 0.52 +0.5 2,624,881 - Psyr_2260 0.52 +0.3 2,624,882 + Psyr_2260 0.52 -1.2 2,624,882 + Psyr_2260 0.52 +0.7 2,624,883 - Psyr_2260 0.52 +0.6 2,625,636 + -1.1 2,625,637 - -1.0 2,625,640 + +0.7 2,625,640 + +0.2 2,625,641 - -1.7 2,625,641 - -0.3 2,625,642 + -1.5 2,625,643 - +0.4 2,625,659 + -0.7 2,625,660 - -0.1 2,625,665 + +0.1 2,625,670 + +0.4 2,625,955 - Psyr_2261 0.19 +0.6 2,625,955 - Psyr_2261 0.19 -0.8 2,625,958 + Psyr_2261 0.19 +3.0 2,625,958 + Psyr_2261 0.19 -0.3 2,625,959 - Psyr_2261 0.20 -0.5 2,625,959 - Psyr_2261 0.20 -1.0 2,625,959 - Psyr_2261 0.20 -0.4 2,626,076 + Psyr_2261 0.30 -1.0 2,626,076 + Psyr_2261 0.30 -0.6 2,626,156 + Psyr_2261 0.37 +0.3 2,626,226 + Psyr_2261 0.43 +0.2 2,626,226 + Psyr_2261 0.43 -0.1 2,626,227 - Psyr_2261 0.43 -2.8
Or see this region's nucleotide sequence