Strain Fitness in Escherichia coli BW25113 around b4233

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfbp and mpl are separated by 175 nucleotidesmpl and yjgA are separated by 155 nucleotidesyjgA and pmbA are separated by 93 nucleotides b4232: fbp - fructose-1,6-bisphosphatase (NCBI), at 4,452,634 to 4,453,632 fbp b4233: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI), at 4,453,808 to 4,455,181 mpl b4234: yjgA - hypothetical protein (NCBI), at 4,455,337 to 4,455,888 yjgA b4235: pmbA - predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI), at 4,455,982 to 4,457,334 pmbA Position (kb) 4453 4454 4455 4456Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4452.867 kb on - strand, within fbpat 4452.869 kb on - strand, within fbpat 4452.869 kb on - strand, within fbpat 4452.906 kb on + strand, within fbpat 4452.926 kb on + strand, within fbpat 4452.934 kb on - strand, within fbpat 4452.971 kb on + strand, within fbpat 4452.989 kb on + strand, within fbpat 4452.989 kb on + strand, within fbpat 4453.022 kb on - strand, within fbpat 4453.102 kb on + strand, within fbpat 4453.105 kb on - strand, within fbpat 4453.132 kb on - strand, within fbpat 4453.139 kb on + strand, within fbpat 4453.147 kb on + strand, within fbpat 4453.147 kb on + strand, within fbpat 4453.147 kb on + strand, within fbpat 4453.155 kb on - strand, within fbpat 4453.155 kb on - strand, within fbpat 4453.226 kb on + strand, within fbpat 4453.289 kb on + strand, within fbpat 4453.289 kb on + strand, within fbpat 4453.289 kb on + strand, within fbpat 4453.306 kb on + strand, within fbpat 4453.314 kb on - strand, within fbpat 4453.368 kb on - strand, within fbpat 4453.368 kb on - strand, within fbpat 4453.368 kb on - strand, within fbpat 4453.374 kb on - strand, within fbpat 4453.380 kb on - strand, within fbpat 4453.380 kb on - strand, within fbpat 4453.391 kb on - strand, within fbpat 4453.456 kb on + strand, within fbpat 4453.456 kb on + strand, within fbpat 4453.508 kb on - strand, within fbpat 4453.518 kb on - strand, within fbpat 4453.532 kb on - strand, within fbpat 4453.562 kb on + strandat 4453.569 kb on + strandat 4453.580 kb on - strandat 4453.580 kb on - strandat 4453.892 kb on - strandat 4454.033 kb on - strand, within mplat 4454.066 kb on + strand, within mplat 4454.066 kb on + strand, within mplat 4454.072 kb on - strand, within mplat 4454.082 kb on - strand, within mplat 4454.082 kb on - strand, within mplat 4454.086 kb on - strand, within mplat 4454.087 kb on - strand, within mplat 4454.087 kb on - strand, within mplat 4454.112 kb on + strand, within mplat 4454.120 kb on + strand, within mplat 4454.141 kb on - strand, within mplat 4454.141 kb on - strand, within mplat 4454.141 kb on - strand, within mplat 4454.143 kb on + strand, within mplat 4454.178 kb on - strand, within mplat 4454.186 kb on - strand, within mplat 4454.288 kb on + strand, within mplat 4454.296 kb on - strand, within mplat 4454.296 kb on - strand, within mplat 4454.317 kb on + strand, within mplat 4454.317 kb on + strand, within mplat 4454.348 kb on - strand, within mplat 4454.385 kb on + strand, within mplat 4454.506 kb on + strand, within mplat 4454.508 kb on + strand, within mplat 4454.508 kb on + strand, within mplat 4454.624 kb on - strand, within mplat 4454.624 kb on - strand, within mplat 4454.697 kb on + strand, within mplat 4454.783 kb on + strand, within mplat 4454.896 kb on - strand, within mplat 4454.932 kb on + strand, within mplat 4454.944 kb on - strand, within mplat 4454.944 kb on - strand, within mplat 4454.968 kb on - strand, within mplat 4455.002 kb on - strand, within mplat 4455.020 kb on - strand, within mplat 4455.031 kb on - strand, within mplat 4455.047 kb on - strandat 4455.067 kb on + strandat 4455.067 kb on + strandat 4455.093 kb on + strandat 4455.129 kb on + strandat 4455.262 kb on - strandat 4455.351 kb on + strandat 4455.469 kb on - strand, within yjgAat 4455.483 kb on - strand, within yjgAat 4455.582 kb on - strand, within yjgAat 4455.628 kb on - strand, within yjgAat 4455.628 kb on - strand, within yjgAat 4455.695 kb on - strand, within yjgAat 4455.727 kb on - strand, within yjgAat 4455.924 kb on + strandat 4455.960 kb on - strandat 4455.971 kb on + strandat 4456.031 kb on - strandat 4456.031 kb on - strandat 4456.053 kb on - strandat 4456.053 kb on - strandat 4456.054 kb on + strandat 4456.062 kb on - strandat 4456.062 kb on - strandat 4456.083 kb on - strandat 4456.093 kb on - strandat 4456.131 kb on + strand, within pmbAat 4456.132 kb on + strand, within pmbAat 4456.132 kb on + strand, within pmbA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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4,452,867 - fbp b4232 0.23 -0.6
4,452,869 - fbp b4232 0.24 -0.7
4,452,869 - fbp b4232 0.24 -2.7
4,452,906 + fbp b4232 0.27 -2.4
4,452,926 + fbp b4232 0.29 -0.2
4,452,934 - fbp b4232 0.30 -0.4
4,452,971 + fbp b4232 0.34 +1.2
4,452,989 + fbp b4232 0.36 -0.0
4,452,989 + fbp b4232 0.36 +0.6
4,453,022 - fbp b4232 0.39 +0.9
4,453,102 + fbp b4232 0.47 -0.8
4,453,105 - fbp b4232 0.47 -1.9
4,453,132 - fbp b4232 0.50 -0.0
4,453,139 + fbp b4232 0.51 +0.3
4,453,147 + fbp b4232 0.51 -0.7
4,453,147 + fbp b4232 0.51 -1.8
4,453,147 + fbp b4232 0.51 -2.0
4,453,155 - fbp b4232 0.52 -1.5
4,453,155 - fbp b4232 0.52 -0.3
4,453,226 + fbp b4232 0.59 -0.3
4,453,289 + fbp b4232 0.66 -1.1
4,453,289 + fbp b4232 0.66 -1.0
4,453,289 + fbp b4232 0.66 -1.4
4,453,306 + fbp b4232 0.67 -1.3
4,453,314 - fbp b4232 0.68 +1.2
4,453,368 - fbp b4232 0.73 -2.1
4,453,368 - fbp b4232 0.73 +0.5
4,453,368 - fbp b4232 0.73 +1.2
4,453,374 - fbp b4232 0.74 -1.3
4,453,380 - fbp b4232 0.75 -2.0
4,453,380 - fbp b4232 0.75 +1.3
4,453,391 - fbp b4232 0.76 +0.0
4,453,456 + fbp b4232 0.82 +1.5
4,453,456 + fbp b4232 0.82 -1.4
4,453,508 - fbp b4232 0.87 +0.4
4,453,518 - fbp b4232 0.88 +0.4
4,453,532 - fbp b4232 0.90 -1.6
4,453,562 + -1.0
4,453,569 + +0.6
4,453,580 - -1.1
4,453,580 - -0.1
4,453,892 - -1.8
4,454,033 - mpl b4233 0.16 +0.3
4,454,066 + mpl b4233 0.19 +0.6
4,454,066 + mpl b4233 0.19 -2.3
4,454,072 - mpl b4233 0.19 -1.8
4,454,082 - mpl b4233 0.20 +0.0
4,454,082 - mpl b4233 0.20 -0.5
4,454,086 - mpl b4233 0.20 +0.5
4,454,087 - mpl b4233 0.20 +0.7
4,454,087 - mpl b4233 0.20 -1.5
4,454,112 + mpl b4233 0.22 +0.2
4,454,120 + mpl b4233 0.23 -0.1
4,454,141 - mpl b4233 0.24 +0.4
4,454,141 - mpl b4233 0.24 -1.1
4,454,141 - mpl b4233 0.24 -0.7
4,454,143 + mpl b4233 0.24 -0.0
4,454,178 - mpl b4233 0.27 -0.9
4,454,186 - mpl b4233 0.28 +0.3
4,454,288 + mpl b4233 0.35 -0.3
4,454,296 - mpl b4233 0.36 -2.0
4,454,296 - mpl b4233 0.36 -0.1
4,454,317 + mpl b4233 0.37 +0.4
4,454,317 + mpl b4233 0.37 -0.0
4,454,348 - mpl b4233 0.39 -0.6
4,454,385 + mpl b4233 0.42 -1.0
4,454,506 + mpl b4233 0.51 +0.5
4,454,508 + mpl b4233 0.51 -1.8
4,454,508 + mpl b4233 0.51 +0.1
4,454,624 - mpl b4233 0.59 -0.2
4,454,624 - mpl b4233 0.59 -1.3
4,454,697 + mpl b4233 0.65 -0.0
4,454,783 + mpl b4233 0.71 -2.3
4,454,896 - mpl b4233 0.79 -0.3
4,454,932 + mpl b4233 0.82 -0.1
4,454,944 - mpl b4233 0.83 -0.1
4,454,944 - mpl b4233 0.83 -0.2
4,454,968 - mpl b4233 0.84 -0.4
4,455,002 - mpl b4233 0.87 -0.2
4,455,020 - mpl b4233 0.88 -0.1
4,455,031 - mpl b4233 0.89 -1.4
4,455,047 - -2.1
4,455,067 + +0.4
4,455,067 + +0.6
4,455,093 + -0.2
4,455,129 + +0.6
4,455,262 - -2.4
4,455,351 + -2.1
4,455,469 - yjgA b4234 0.24 -0.1
4,455,483 - yjgA b4234 0.26 -1.4
4,455,582 - yjgA b4234 0.44 +0.0
4,455,628 - yjgA b4234 0.53 -1.6
4,455,628 - yjgA b4234 0.53 +1.8
4,455,695 - yjgA b4234 0.65 -1.1
4,455,727 - yjgA b4234 0.71 -0.5
4,455,924 + -0.4
4,455,960 - +1.1
4,455,971 + -0.5
4,456,031 - -0.1
4,456,031 - -1.8
4,456,053 - -2.6
4,456,053 - +0.7
4,456,054 + -3.2
4,456,062 - -0.9
4,456,062 - -1.2
4,456,083 - -1.2
4,456,093 - +1.2
4,456,131 + pmbA b4235 0.11 -0.8
4,456,132 + pmbA b4235 0.11 -0.7
4,456,132 + pmbA b4235 0.11 -0.6

Or see this region's nucleotide sequence