Strain Fitness in Escherichia coli BW25113 around b3650

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgmk and rpoZ are separated by 54 nucleotidesrpoZ and spoT are separated by 18 nucleotidesspoT and trmH are separated by 6 nucleotidestrmH and recG are separated by 5 nucleotides b3648: gmk - guanylate kinase (NCBI), at 3,819,451 to 3,820,074 gmk b3649: rpoZ - DNA-directed RNA polymerase subunit omega (NCBI), at 3,820,129 to 3,820,404 rpoZ b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531 spoT b3651: trmH - tRNA (Guanosine-2'-O-)-methyltransferase (NCBI), at 3,822,538 to 3,823,227 trmH b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314 recG Position (kb) 3820 3821 3822 3823Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3820.087 kb on - strandat 3820.111 kb on - strandat 3820.144 kb on + strandat 3820.144 kb on + strandat 3820.151 kb on + strandat 3820.205 kb on + strand, within rpoZat 3820.205 kb on + strand, within rpoZat 3820.210 kb on + strand, within rpoZat 3820.297 kb on + strand, within rpoZat 3820.297 kb on + strand, within rpoZat 3820.340 kb on + strand, within rpoZat 3820.341 kb on - strand, within rpoZat 3820.365 kb on + strand, within rpoZat 3820.420 kb on + strandat 3820.420 kb on + strandat 3820.448 kb on - strandat 3820.452 kb on + strandat 3821.855 kb on + strand, within spoTat 3821.855 kb on + strand, within spoTat 3821.879 kb on + strand, within spoTat 3821.920 kb on - strand, within spoTat 3821.939 kb on + strand, within spoTat 3821.957 kb on - strand, within spoTat 3821.957 kb on - strand, within spoTat 3821.972 kb on - strand, within spoTat 3822.086 kb on + strand, within spoTat 3822.104 kb on - strand, within spoTat 3822.116 kb on - strand, within spoTat 3822.174 kb on - strand, within spoTat 3822.214 kb on - strand, within spoTat 3822.220 kb on + strand, within spoTat 3822.220 kb on + strand, within spoTat 3822.222 kb on + strand, within spoTat 3822.244 kb on + strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.397 kb on - strandat 3822.397 kb on - strandat 3822.401 kb on - strandat 3822.409 kb on - strandat 3822.409 kb on - strandat 3822.417 kb on + strandat 3822.455 kb on + strandat 3822.455 kb on + strandat 3822.493 kb on + strandat 3822.493 kb on + strandat 3822.592 kb on + strandat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.618 kb on - strand, within trmHat 3822.635 kb on - strand, within trmHat 3822.733 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.749 kb on - strand, within trmHat 3822.761 kb on - strand, within trmHat 3822.778 kb on - strand, within trmHat 3822.796 kb on - strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.807 kb on - strand, within trmHat 3822.922 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.945 kb on - strand, within trmHat 3823.040 kb on - strand, within trmHat 3823.089 kb on - strand, within trmHat 3823.117 kb on - strand, within trmHat 3823.179 kb on + strandat 3823.179 kb on + strandat 3823.189 kb on + strandat 3823.189 kb on + strandat 3823.215 kb on - strandat 3823.215 kb on - strandat 3823.231 kb on - strandat 3823.265 kb on + strandat 3823.267 kb on + strandat 3823.285 kb on + strandat 3823.341 kb on + strandat 3823.341 kb on + strandat 3823.379 kb on + strandat 3823.379 kb on + strandat 3823.387 kb on - strandat 3823.387 kb on - strandat 3823.393 kb on + strandat 3823.439 kb on + strandat 3823.482 kb on - strand, within recG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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3,820,087 - -3.2
3,820,111 - -0.6
3,820,144 + -2.1
3,820,144 + -0.0
3,820,151 + -0.6
3,820,205 + rpoZ b3649 0.28 +2.4
3,820,205 + rpoZ b3649 0.28 -1.2
3,820,210 + rpoZ b3649 0.29 -3.0
3,820,297 + rpoZ b3649 0.61 -0.9
3,820,297 + rpoZ b3649 0.61 -1.4
3,820,340 + rpoZ b3649 0.76 -2.2
3,820,341 - rpoZ b3649 0.77 -1.4
3,820,365 + rpoZ b3649 0.86 -2.1
3,820,420 + -2.1
3,820,420 + -0.5
3,820,448 - -2.2
3,820,452 + -0.3
3,821,855 + spoT b3650 0.68 -0.8
3,821,855 + spoT b3650 0.68 -2.0
3,821,879 + spoT b3650 0.69 -3.0
3,821,920 - spoT b3650 0.71 +0.4
3,821,939 + spoT b3650 0.72 -1.9
3,821,957 - spoT b3650 0.73 +0.4
3,821,957 - spoT b3650 0.73 +0.4
3,821,972 - spoT b3650 0.73 +0.1
3,822,086 + spoT b3650 0.79 -0.5
3,822,104 - spoT b3650 0.80 -2.2
3,822,116 - spoT b3650 0.80 -0.8
3,822,174 - spoT b3650 0.83 -0.2
3,822,214 - spoT b3650 0.85 -0.2
3,822,220 + spoT b3650 0.85 +0.8
3,822,220 + spoT b3650 0.85 -2.1
3,822,222 + spoT b3650 0.85 -2.8
3,822,244 + spoT b3650 0.86 +0.4
3,822,263 - spoT b3650 0.87 -1.7
3,822,263 - spoT b3650 0.87 -0.9
3,822,397 - -1.4
3,822,397 - -0.6
3,822,401 - -1.4
3,822,409 - -0.6
3,822,409 - -0.6
3,822,417 + -1.2
3,822,455 + -1.6
3,822,455 + +1.8
3,822,493 + -0.2
3,822,493 + -2.1
3,822,592 + +0.3
3,822,615 + trmH b3651 0.11 -1.1
3,822,615 + trmH b3651 0.11 +1.1
3,822,615 + trmH b3651 0.11 +0.6
3,822,618 - trmH b3651 0.12 -0.4
3,822,635 - trmH b3651 0.14 +0.8
3,822,733 + trmH b3651 0.28 +0.2
3,822,746 + trmH b3651 0.30 -1.5
3,822,746 + trmH b3651 0.30 -1.1
3,822,749 - trmH b3651 0.31 -0.6
3,822,761 - trmH b3651 0.32 -1.7
3,822,778 - trmH b3651 0.35 -1.7
3,822,796 - trmH b3651 0.37 -1.5
3,822,799 + trmH b3651 0.38 +0.3
3,822,799 + trmH b3651 0.38 -0.1
3,822,807 - trmH b3651 0.39 -1.5
3,822,922 - trmH b3651 0.56 -0.9
3,822,930 - trmH b3651 0.57 +0.7
3,822,930 - trmH b3651 0.57 -1.8
3,822,945 - trmH b3651 0.59 -0.6
3,823,040 - trmH b3651 0.73 -0.4
3,823,089 - trmH b3651 0.80 -2.6
3,823,117 - trmH b3651 0.84 -2.2
3,823,179 + +0.8
3,823,179 + -0.2
3,823,189 + -1.2
3,823,189 + +1.4
3,823,215 - -2.7
3,823,215 - -1.8
3,823,231 - -1.3
3,823,265 + +0.8
3,823,267 + +1.4
3,823,285 + -0.6
3,823,341 + -0.9
3,823,341 + +1.4
3,823,379 + -1.4
3,823,379 + +1.4
3,823,387 - -1.9
3,823,387 - -2.3
3,823,393 + -0.9
3,823,439 + -1.9
3,823,482 - recG b3652 0.12 +0.4

Or see this region's nucleotide sequence