Experiment: LB Anaerobic with Furaltadone 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frlD and yhfR are separated by 99 nucleotides yhfR and yhfS are separated by 151 nucleotides yhfS and yhfT are separated by 11 nucleotides
b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974
frlD
b3375: yhfR - putative transcriptional regulator (VIMSS), at 3,502,074 to 3,502,805
yhfR
b3376: yhfS - hypothetical protein (NCBI), at 3,502,957 to 3,504,042
yhfS
b3377: yhfT - predicted inner membrane protein (NCBI), at 3,504,054 to 3,505,358
yhfT
Position (kb)
3502
3503
3504
3505 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3501.962 kb on - strand at 3501.964 kb on - strand at 3501.975 kb on + strand at 3501.975 kb on + strand at 3501.980 kb on + strand at 3501.998 kb on - strand at 3501.998 kb on - strand at 3502.109 kb on + strand at 3502.115 kb on - strand at 3502.147 kb on + strand at 3502.157 kb on + strand, within yhfR at 3502.161 kb on - strand, within yhfR at 3502.175 kb on - strand, within yhfR at 3502.205 kb on + strand, within yhfR at 3502.205 kb on + strand, within yhfR at 3502.244 kb on - strand, within yhfR at 3502.244 kb on - strand, within yhfR at 3502.269 kb on - strand, within yhfR at 3502.319 kb on - strand, within yhfR at 3502.326 kb on + strand, within yhfR at 3502.333 kb on - strand, within yhfR at 3502.333 kb on - strand, within yhfR at 3502.337 kb on - strand, within yhfR at 3502.337 kb on - strand, within yhfR at 3502.393 kb on + strand, within yhfR at 3502.401 kb on - strand, within yhfR at 3502.403 kb on + strand, within yhfR at 3502.403 kb on + strand, within yhfR at 3502.403 kb on + strand, within yhfR at 3502.403 kb on + strand, within yhfR at 3502.405 kb on - strand, within yhfR at 3502.411 kb on - strand, within yhfR at 3502.518 kb on + strand, within yhfR at 3502.518 kb on - strand, within yhfR at 3502.518 kb on - strand, within yhfR at 3502.518 kb on - strand at 3502.529 kb on + strand, within yhfR at 3502.710 kb on + strand, within yhfR at 3502.734 kb on + strand at 3502.879 kb on + strand at 3502.884 kb on + strand at 3502.893 kb on - strand at 3502.894 kb on - strand at 3502.894 kb on - strand at 3502.896 kb on + strand at 3502.924 kb on + strand at 3502.924 kb on + strand at 3502.944 kb on + strand at 3502.952 kb on - strand at 3502.957 kb on - strand at 3502.975 kb on - strand at 3502.975 kb on - strand at 3502.987 kb on + strand at 3503.157 kb on + strand, within yhfS at 3503.157 kb on + strand, within yhfS at 3503.166 kb on + strand, within yhfS at 3503.166 kb on + strand, within yhfS at 3503.217 kb on + strand, within yhfS at 3503.217 kb on + strand, within yhfS at 3503.248 kb on - strand, within yhfS at 3503.257 kb on + strand, within yhfS at 3503.258 kb on + strand, within yhfS at 3503.260 kb on - strand, within yhfS at 3503.260 kb on - strand, within yhfS at 3503.266 kb on - strand, within yhfS at 3503.266 kb on - strand, within yhfS at 3503.278 kb on - strand, within yhfS at 3503.305 kb on + strand, within yhfS at 3503.330 kb on - strand, within yhfS at 3503.330 kb on - strand, within yhfS at 3503.364 kb on - strand, within yhfS at 3503.378 kb on + strand, within yhfS at 3503.386 kb on - strand, within yhfS at 3503.386 kb on - strand, within yhfS at 3503.409 kb on + strand, within yhfS at 3503.409 kb on + strand, within yhfS at 3503.427 kb on + strand, within yhfS at 3503.427 kb on + strand, within yhfS at 3503.510 kb on + strand, within yhfS at 3503.520 kb on + strand, within yhfS at 3503.527 kb on + strand, within yhfS at 3503.588 kb on + strand, within yhfS at 3503.604 kb on - strand, within yhfS at 3503.718 kb on + strand, within yhfS at 3503.737 kb on + strand, within yhfS at 3503.760 kb on + strand, within yhfS at 3503.824 kb on - strand, within yhfS at 3503.824 kb on - strand, within yhfS at 3503.827 kb on - strand, within yhfS at 3503.871 kb on - strand, within yhfS at 3503.890 kb on - strand, within yhfS at 3503.890 kb on - strand, within yhfS at 3503.928 kb on - strand, within yhfS at 3504.066 kb on + strand at 3504.118 kb on + strand at 3504.118 kb on + strand at 3504.146 kb on + strand at 3504.148 kb on + strand at 3504.153 kb on + strand at 3504.153 kb on + strand at 3504.153 kb on + strand at 3504.153 kb on + strand at 3504.153 kb on + strand at 3504.161 kb on - strand at 3504.244 kb on + strand, within yhfT at 3504.277 kb on + strand, within yhfT at 3504.331 kb on - strand, within yhfT at 3504.375 kb on + strand, within yhfT at 3504.383 kb on - strand, within yhfT at 3504.383 kb on - strand, within yhfT at 3504.448 kb on + strand, within yhfT at 3504.450 kb on + strand, within yhfT at 3504.450 kb on + strand, within yhfT at 3504.453 kb on - strand, within yhfT at 3504.458 kb on - strand, within yhfT at 3504.458 kb on - strand, within yhfT at 3504.458 kb on - strand, within yhfT at 3504.515 kb on + strand, within yhfT at 3504.537 kb on - strand, within yhfT at 3504.537 kb on - strand, within yhfT at 3504.747 kb on + strand, within yhfT at 3504.747 kb on + strand, within yhfT at 3504.763 kb on - strand, within yhfT at 3504.774 kb on + strand, within yhfT at 3504.774 kb on + strand, within yhfT at 3504.837 kb on - strand, within yhfT at 3504.855 kb on + strand, within yhfT at 3504.855 kb on + strand, within yhfT at 3504.871 kb on - strand, within yhfT at 3504.871 kb on - strand, within yhfT at 3504.887 kb on - strand, within yhfT at 3504.887 kb on - strand, within yhfT at 3504.887 kb on - strand, within yhfT at 3504.932 kb on + strand, within yhfT at 3504.932 kb on + strand at 3504.985 kb on + strand, within yhfT at 3504.985 kb on + strand, within yhfT at 3505.002 kb on + strand, within yhfT at 3505.025 kb on + strand, within yhfT at 3505.035 kb on + strand, within yhfT
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM remove 3,501,962 - -1.2 3,501,964 - -0.3 3,501,975 + -0.2 3,501,975 + +0.8 3,501,980 + -0.5 3,501,998 - +2.1 3,501,998 - -0.0 3,502,109 + -1.3 3,502,115 - -0.7 3,502,147 + -1.2 3,502,157 + yhfR b3375 0.11 -2.1 3,502,161 - yhfR b3375 0.12 -0.3 3,502,175 - yhfR b3375 0.14 -0.9 3,502,205 + yhfR b3375 0.18 -1.1 3,502,205 + yhfR b3375 0.18 +0.4 3,502,244 - yhfR b3375 0.23 -1.3 3,502,244 - yhfR b3375 0.23 -1.1 3,502,269 - yhfR b3375 0.27 -0.5 3,502,319 - yhfR b3375 0.33 -0.6 3,502,326 + yhfR b3375 0.34 -0.6 3,502,333 - yhfR b3375 0.35 -0.9 3,502,333 - yhfR b3375 0.35 -1.7 3,502,337 - yhfR b3375 0.36 +1.3 3,502,337 - yhfR b3375 0.36 -0.3 3,502,393 + yhfR b3375 0.44 -0.6 3,502,401 - yhfR b3375 0.45 -1.1 3,502,403 + yhfR b3375 0.45 -1.4 3,502,403 + yhfR b3375 0.45 -1.6 3,502,403 + yhfR b3375 0.45 +0.4 3,502,403 + yhfR b3375 0.45 -1.5 3,502,405 - yhfR b3375 0.45 -0.5 3,502,411 - yhfR b3375 0.46 +0.7 3,502,518 + yhfR b3375 0.61 -2.0 3,502,518 - yhfR b3375 0.61 -0.2 3,502,518 - yhfR b3375 0.61 +0.3 3,502,518 - +0.7 3,502,529 + yhfR b3375 0.62 -1.2 3,502,710 + yhfR b3375 0.87 -0.7 3,502,734 + -0.3 3,502,879 + +0.9 3,502,884 + -2.4 3,502,893 - +2.0 3,502,894 - +0.1 3,502,894 - +0.4 3,502,896 + -0.6 3,502,924 + -0.5 3,502,924 + -0.6 3,502,944 + -0.5 3,502,952 - -1.9 3,502,957 - -0.3 3,502,975 - +0.3 3,502,975 - -0.4 3,502,987 + -1.8 3,503,157 + yhfS b3376 0.18 -0.9 3,503,157 + yhfS b3376 0.18 -1.7 3,503,166 + yhfS b3376 0.19 -1.5 3,503,166 + yhfS b3376 0.19 -1.8 3,503,217 + yhfS b3376 0.24 -1.5 3,503,217 + yhfS b3376 0.24 -0.3 3,503,248 - yhfS b3376 0.27 +1.1 3,503,257 + yhfS b3376 0.28 -1.3 3,503,258 + yhfS b3376 0.28 -0.0 3,503,260 - yhfS b3376 0.28 -1.2 3,503,260 - yhfS b3376 0.28 +0.1 3,503,266 - yhfS b3376 0.28 -0.7 3,503,266 - yhfS b3376 0.28 +0.5 3,503,278 - yhfS b3376 0.30 -2.3 3,503,305 + yhfS b3376 0.32 +0.4 3,503,330 - yhfS b3376 0.34 +0.0 3,503,330 - yhfS b3376 0.34 +0.3 3,503,364 - yhfS b3376 0.37 +0.9 3,503,378 + yhfS b3376 0.39 -0.0 3,503,386 - yhfS b3376 0.40 -1.1 3,503,386 - yhfS b3376 0.40 -0.5 3,503,409 + yhfS b3376 0.42 -0.4 3,503,409 + yhfS b3376 0.42 -1.7 3,503,427 + yhfS b3376 0.43 -0.1 3,503,427 + yhfS b3376 0.43 +0.3 3,503,510 + yhfS b3376 0.51 +0.3 3,503,520 + yhfS b3376 0.52 -0.7 3,503,527 + yhfS b3376 0.52 -0.8 3,503,588 + yhfS b3376 0.58 +0.6 3,503,604 - yhfS b3376 0.60 -1.8 3,503,718 + yhfS b3376 0.70 -0.8 3,503,737 + yhfS b3376 0.72 -1.1 3,503,760 + yhfS b3376 0.74 +1.7 3,503,824 - yhfS b3376 0.80 +0.8 3,503,824 - yhfS b3376 0.80 -0.7 3,503,827 - yhfS b3376 0.80 +0.4 3,503,871 - yhfS b3376 0.84 -0.5 3,503,890 - yhfS b3376 0.86 +0.4 3,503,890 - yhfS b3376 0.86 -0.2 3,503,928 - yhfS b3376 0.89 +0.4 3,504,066 + -1.6 3,504,118 + +1.6 3,504,118 + -0.4 3,504,146 + -1.1 3,504,148 + -0.5 3,504,153 + -0.8 3,504,153 + -0.7 3,504,153 + -0.4 3,504,153 + +0.7 3,504,153 + +0.3 3,504,161 - -0.7 3,504,244 + yhfT b3377 0.15 -0.4 3,504,277 + yhfT b3377 0.17 +0.4 3,504,331 - yhfT b3377 0.21 +0.2 3,504,375 + yhfT b3377 0.25 -2.2 3,504,383 - yhfT b3377 0.25 +3.1 3,504,383 - yhfT b3377 0.25 +0.6 3,504,448 + yhfT b3377 0.30 -1.3 3,504,450 + yhfT b3377 0.30 -0.9 3,504,450 + yhfT b3377 0.30 -1.3 3,504,453 - yhfT b3377 0.31 -1.3 3,504,458 - yhfT b3377 0.31 -1.1 3,504,458 - yhfT b3377 0.31 -0.1 3,504,458 - yhfT b3377 0.31 -0.9 3,504,515 + yhfT b3377 0.35 -0.6 3,504,537 - yhfT b3377 0.37 -2.0 3,504,537 - yhfT b3377 0.37 +1.4 3,504,747 + yhfT b3377 0.53 +0.3 3,504,747 + yhfT b3377 0.53 +1.4 3,504,763 - yhfT b3377 0.54 +0.5 3,504,774 + yhfT b3377 0.55 -1.3 3,504,774 + yhfT b3377 0.55 -1.5 3,504,837 - yhfT b3377 0.60 -1.1 3,504,855 + yhfT b3377 0.61 +0.7 3,504,855 + yhfT b3377 0.61 -0.7 3,504,871 - yhfT b3377 0.63 +2.6 3,504,871 - yhfT b3377 0.63 -1.3 3,504,887 - yhfT b3377 0.64 -0.6 3,504,887 - yhfT b3377 0.64 -1.5 3,504,887 - yhfT b3377 0.64 +0.3 3,504,932 + yhfT b3377 0.67 +0.3 3,504,932 + -1.2 3,504,985 + yhfT b3377 0.71 +0.1 3,504,985 + yhfT b3377 0.71 -0.7 3,505,002 + yhfT b3377 0.73 -0.2 3,505,025 + yhfT b3377 0.74 +0.8 3,505,035 + yhfT b3377 0.75 +0.3
Or see this region's nucleotide sequence