Strain Fitness in Escherichia coli BW25113 around b2458

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutE and cchB are separated by 11 nucleotidescchB and cchA are separated by 106 nucleotidescchA and eutI are separated by 38 nucleotideseutI and eutT overlap by 4 nucleotideseutT and eutQ overlap by 4 nucleotides b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773 eutE b2456: cchB - predicted carboxysome structural protein, ethanolamine utilization protein (NCBI), at 2,569,785 to 2,570,072 cchB b2457: cchA - detox protein (VIMSS), at 2,570,179 to 2,570,472 cchA b2458: eutI - predicted phosphotransacetylase subunit (NCBI), at 2,570,511 to 2,571,527 eutI b2459: eutT - predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI), at 2,571,524 to 2,572,327 eutT b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025 eutQ Position (kb) 2570 2571 2572Strain fitness (log2 ratio) -2 -1 0 1 2at 2569.734 kb on + strandat 2569.820 kb on + strand, within cchBat 2569.837 kb on + strand, within cchBat 2569.849 kb on - strand, within cchBat 2569.849 kb on - strand, within cchBat 2570.117 kb on - strandat 2570.168 kb on + strandat 2570.168 kb on + strandat 2570.261 kb on - strand, within cchAat 2570.318 kb on - strand, within cchAat 2570.350 kb on + strand, within cchAat 2570.353 kb on - strand, within cchAat 2570.353 kb on - strand, within cchAat 2570.368 kb on - strand, within cchAat 2570.435 kb on + strand, within cchAat 2570.435 kb on + strand, within cchAat 2570.490 kb on - strandat 2570.502 kb on - strandat 2570.654 kb on - strand, within eutIat 2570.989 kb on + strand, within eutIat 2570.989 kb on + strand, within eutIat 2571.039 kb on + strand, within eutIat 2571.065 kb on - strand, within eutIat 2571.148 kb on + strand, within eutIat 2571.148 kb on + strand, within eutIat 2571.330 kb on - strand, within eutIat 2571.337 kb on - strand, within eutIat 2571.337 kb on - strand, within eutIat 2571.441 kb on + strandat 2571.561 kb on + strandat 2571.561 kb on + strandat 2571.564 kb on - strandat 2571.599 kb on + strandat 2571.603 kb on - strandat 2571.603 kb on - strandat 2571.655 kb on + strand, within eutTat 2571.812 kb on + strand, within eutTat 2571.924 kb on + strand, within eutTat 2571.924 kb on + strand, within eutTat 2571.985 kb on - strand, within eutTat 2571.985 kb on - strand, within eutTat 2572.113 kb on + strand, within eutTat 2572.134 kb on + strand, within eutTat 2572.134 kb on + strand, within eutTat 2572.153 kb on - strand, within eutTat 2572.405 kb on + strand, within eutQat 2572.471 kb on - strand, within eutQat 2572.471 kb on - strand, within eutQat 2572.477 kb on + strand, within eutQ

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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2,569,734 + -1.6
2,569,820 + cchB b2456 0.12 -0.4
2,569,837 + cchB b2456 0.18 -0.4
2,569,849 - cchB b2456 0.22 -1.4
2,569,849 - cchB b2456 0.22 -1.4
2,570,117 - -0.3
2,570,168 + +1.3
2,570,168 + +0.2
2,570,261 - cchA b2457 0.28 +0.3
2,570,318 - cchA b2457 0.47 +1.2
2,570,350 + cchA b2457 0.58 +2.7
2,570,353 - cchA b2457 0.59 -1.5
2,570,353 - cchA b2457 0.59 -0.0
2,570,368 - cchA b2457 0.64 +0.3
2,570,435 + cchA b2457 0.87 -0.6
2,570,435 + cchA b2457 0.87 +1.0
2,570,490 - -0.8
2,570,502 - +0.8
2,570,654 - eutI b2458 0.14 -0.1
2,570,989 + eutI b2458 0.47 +0.3
2,570,989 + eutI b2458 0.47 -0.4
2,571,039 + eutI b2458 0.52 +2.0
2,571,065 - eutI b2458 0.54 +0.2
2,571,148 + eutI b2458 0.63 +0.0
2,571,148 + eutI b2458 0.63 +0.4
2,571,330 - eutI b2458 0.81 +1.1
2,571,337 - eutI b2458 0.81 -0.7
2,571,337 - eutI b2458 0.81 +0.5
2,571,441 + -0.4
2,571,561 + -0.2
2,571,561 + -1.2
2,571,564 - +0.6
2,571,599 + -0.3
2,571,603 - +0.2
2,571,603 - -0.1
2,571,655 + eutT b2459 0.16 -0.4
2,571,812 + eutT b2459 0.36 +1.2
2,571,924 + eutT b2459 0.50 -0.8
2,571,924 + eutT b2459 0.50 +0.3
2,571,985 - eutT b2459 0.57 -2.7
2,571,985 - eutT b2459 0.57 -1.0
2,572,113 + eutT b2459 0.73 +1.9
2,572,134 + eutT b2459 0.76 -1.3
2,572,134 + eutT b2459 0.76 -0.8
2,572,153 - eutT b2459 0.78 +0.9
2,572,405 + eutQ b2460 0.12 -0.2
2,572,471 - eutQ b2460 0.21 -0.3
2,572,471 - eutQ b2460 0.21 -1.7
2,572,477 + eutQ b2460 0.22 -0.0

Or see this region's nucleotide sequence