Strain Fitness in Escherichia coli BW25113 around b2454

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutG and eutJ overlap by 11 nucleotideseutJ and eutE are separated by 10 nucleotides b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533 eutG b2454: eutJ - predicted chaperonin, ethanolamine utilization protein (NCBI), at 2,567,523 to 2,568,359 eutJ b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773 eutE Position (kb) 2567 2568 2569Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2566.585 kb on + strand, within eutGat 2566.620 kb on + strand, within eutGat 2566.620 kb on + strand, within eutGat 2566.637 kb on + strand, within eutGat 2566.894 kb on + strand, within eutGat 2566.914 kb on + strand, within eutGat 2566.938 kb on - strand, within eutGat 2566.938 kb on - strand, within eutGat 2566.938 kb on - strand, within eutGat 2567.008 kb on + strand, within eutGat 2567.012 kb on - strand, within eutGat 2567.130 kb on + strand, within eutGat 2567.210 kb on + strand, within eutGat 2567.220 kb on + strand, within eutGat 2567.220 kb on + strand, within eutGat 2567.241 kb on + strand, within eutGat 2567.241 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.325 kb on - strand, within eutGat 2567.341 kb on + strand, within eutGat 2567.341 kb on + strand, within eutGat 2567.418 kb on - strandat 2567.595 kb on + strandat 2567.707 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.785 kb on + strand, within eutJat 2567.963 kb on + strand, within eutJat 2568.118 kb on - strand, within eutJat 2568.140 kb on - strand, within eutJat 2568.149 kb on + strand, within eutJat 2568.149 kb on + strand, within eutJat 2568.219 kb on + strand, within eutJat 2568.298 kb on + strandat 2568.338 kb on + strandat 2568.891 kb on - strand, within eutEat 2568.932 kb on + strand, within eutEat 2568.932 kb on + strand, within eutEat 2568.937 kb on + strand, within eutEat 2568.937 kb on + strand, within eutEat 2569.130 kb on + strand, within eutEat 2569.252 kb on - strand, within eutEat 2569.252 kb on - strand, within eutE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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2,566,585 + eutG b2453 0.20 +0.3
2,566,620 + eutG b2453 0.23 +0.4
2,566,620 + eutG b2453 0.23 +0.1
2,566,637 + eutG b2453 0.24 +0.4
2,566,894 + eutG b2453 0.46 +0.5
2,566,914 + eutG b2453 0.48 -0.1
2,566,938 - eutG b2453 0.50 -2.2
2,566,938 - eutG b2453 0.50 -0.5
2,566,938 - eutG b2453 0.50 +1.9
2,567,008 + eutG b2453 0.56 -0.5
2,567,012 - eutG b2453 0.56 -0.3
2,567,130 + eutG b2453 0.66 +1.7
2,567,210 + eutG b2453 0.73 -0.9
2,567,220 + eutG b2453 0.74 +0.1
2,567,220 + eutG b2453 0.74 +1.8
2,567,241 + eutG b2453 0.75 -0.5
2,567,241 - eutG b2453 0.75 +1.8
2,567,325 - eutG b2453 0.82 -0.5
2,567,325 - eutG b2453 0.82 -0.4
2,567,325 - eutG b2453 0.82 -0.7
2,567,325 - eutG b2453 0.82 +0.3
2,567,341 + eutG b2453 0.84 -0.3
2,567,341 + eutG b2453 0.84 -0.4
2,567,418 - -0.5
2,567,595 + +0.6
2,567,707 + eutJ b2454 0.22 +0.6
2,567,785 + eutJ b2454 0.31 -0.3
2,567,785 + eutJ b2454 0.31 +1.0
2,567,785 + eutJ b2454 0.31 +1.1
2,567,963 + eutJ b2454 0.53 -0.0
2,568,118 - eutJ b2454 0.71 -0.7
2,568,140 - eutJ b2454 0.74 -3.2
2,568,149 + eutJ b2454 0.75 -0.1
2,568,149 + eutJ b2454 0.75 +0.3
2,568,219 + eutJ b2454 0.83 -0.2
2,568,298 + -1.4
2,568,338 + +0.8
2,568,891 - eutE b2455 0.37 +1.1
2,568,932 + eutE b2455 0.40 -0.6
2,568,932 + eutE b2455 0.40 +1.4
2,568,937 + eutE b2455 0.40 +0.6
2,568,937 + eutE b2455 0.40 -0.4
2,569,130 + eutE b2455 0.54 +0.1
2,569,252 - eutE b2455 0.63 -1.2
2,569,252 - eutE b2455 0.63 +1.2

Or see this region's nucleotide sequence