Strain Fitness in Escherichia coli BW25113 around b2727

Experiment: LB Anaerobic with Chloramphenicol 0.000001953125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycA and hypA are separated by 211 nucleotideshypA and hypB are separated by 3 nucleotideshypB and hypC overlap by 10 nucleotideshypC and hypD overlap by 1 nucleotides b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457 hycA b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019 hypA b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895 hypB b2728: hypC - protein required for maturation of hydrogenases 1 and 3 (NCBI), at 2,849,886 to 2,850,158 hypC b2729: hypD - protein required for maturation of hydrogenases (NCBI), at 2,850,158 to 2,851,279 hypD Position (kb) 2849 2850Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2848.071 kb on - strand, within hycAat 2848.073 kb on - strand, within hycAat 2848.073 kb on - strand, within hycAat 2848.082 kb on - strand, within hycAat 2848.153 kb on - strand, within hycAat 2848.153 kb on - strand, within hycAat 2848.157 kb on + strand, within hycAat 2848.157 kb on + strand, within hycAat 2848.253 kb on - strand, within hycAat 2848.253 kb on - strand, within hycAat 2848.684 kb on - strandat 2848.684 kb on - strandat 2848.684 kb on - strandat 2848.719 kb on - strand, within hypAat 2848.850 kb on - strand, within hypAat 2848.892 kb on + strand, within hypAat 2848.912 kb on + strand, within hypAat 2848.944 kb on + strand, within hypAat 2848.965 kb on - strand, within hypAat 2848.965 kb on - strand, within hypAat 2849.025 kb on + strandat 2849.025 kb on + strandat 2849.290 kb on - strand, within hypBat 2849.290 kb on - strand, within hypBat 2849.345 kb on + strand, within hypBat 2849.390 kb on - strand, within hypBat 2849.430 kb on + strand, within hypBat 2849.430 kb on + strand, within hypBat 2849.486 kb on + strand, within hypBat 2849.486 kb on + strand, within hypBat 2849.494 kb on + strand, within hypBat 2849.507 kb on - strand, within hypBat 2849.581 kb on - strand, within hypBat 2849.748 kb on + strand, within hypBat 2849.884 kb on - strandat 2849.917 kb on + strand, within hypCat 2849.917 kb on + strand, within hypCat 2849.917 kb on - strand, within hypCat 2849.917 kb on - strand, within hypCat 2850.031 kb on - strand, within hypCat 2850.085 kb on + strand, within hypCat 2850.093 kb on - strand, within hypCat 2850.093 kb on - strand, within hypCat 2850.145 kb on - strandat 2850.170 kb on + strandat 2850.170 kb on + strandat 2850.222 kb on + strandat 2850.236 kb on + strandat 2850.246 kb on - strandat 2850.297 kb on - strand, within hypDat 2850.297 kb on - strand, within hypDat 2850.350 kb on - strand, within hypDat 2850.350 kb on - strand, within hypDat 2850.354 kb on + strand, within hypDat 2850.369 kb on - strand, within hypDat 2850.369 kb on - strand, within hypDat 2850.452 kb on + strand, within hypDat 2850.522 kb on - strand, within hypDat 2850.522 kb on - strand, within hypDat 2850.616 kb on - strand, within hypDat 2850.624 kb on - strand, within hypDat 2850.681 kb on + strand, within hypDat 2850.691 kb on - strand, within hypD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chloramphenicol 0.000001953125 mM
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2,848,071 - hycA b2725 0.16 -2.4
2,848,073 - hycA b2725 0.17 +0.4
2,848,073 - hycA b2725 0.17 -0.6
2,848,082 - hycA b2725 0.19 -0.0
2,848,153 - hycA b2725 0.34 +0.5
2,848,153 - hycA b2725 0.34 +1.2
2,848,157 + hycA b2725 0.35 +2.0
2,848,157 + hycA b2725 0.35 +1.0
2,848,253 - hycA b2725 0.56 -0.4
2,848,253 - hycA b2725 0.56 -0.1
2,848,684 - -1.9
2,848,684 - +0.1
2,848,684 - +1.0
2,848,719 - hypA b2726 0.14 +0.3
2,848,850 - hypA b2726 0.52 -0.9
2,848,892 + hypA b2726 0.64 +0.0
2,848,912 + hypA b2726 0.69 +0.7
2,848,944 + hypA b2726 0.78 -1.4
2,848,965 - hypA b2726 0.84 +0.4
2,848,965 - hypA b2726 0.84 +0.1
2,849,025 + -0.4
2,849,025 + -1.5
2,849,290 - hypB b2727 0.31 -0.3
2,849,290 - hypB b2727 0.31 -1.4
2,849,345 + hypB b2727 0.37 -1.4
2,849,390 - hypB b2727 0.42 -0.8
2,849,430 + hypB b2727 0.47 +0.4
2,849,430 + hypB b2727 0.47 -0.6
2,849,486 + hypB b2727 0.53 -1.8
2,849,486 + hypB b2727 0.53 -2.5
2,849,494 + hypB b2727 0.54 -0.3
2,849,507 - hypB b2727 0.55 -2.0
2,849,581 - hypB b2727 0.64 -0.2
2,849,748 + hypB b2727 0.83 -1.4
2,849,884 - -1.2
2,849,917 + hypC b2728 0.11 -0.9
2,849,917 + hypC b2728 0.11 +1.2
2,849,917 - hypC b2728 0.11 -0.5
2,849,917 - hypC b2728 0.11 +0.5
2,850,031 - hypC b2728 0.53 -0.8
2,850,085 + hypC b2728 0.73 +0.2
2,850,093 - hypC b2728 0.76 -3.3
2,850,093 - hypC b2728 0.76 -0.5
2,850,145 - +1.5
2,850,170 + -1.2
2,850,170 + -2.7
2,850,222 + -1.2
2,850,236 + -0.0
2,850,246 - -1.2
2,850,297 - hypD b2729 0.12 +0.8
2,850,297 - hypD b2729 0.12 -1.6
2,850,350 - hypD b2729 0.17 -2.2
2,850,350 - hypD b2729 0.17 -1.5
2,850,354 + hypD b2729 0.17 +0.2
2,850,369 - hypD b2729 0.19 -0.9
2,850,369 - hypD b2729 0.19 -0.5
2,850,452 + hypD b2729 0.26 -1.3
2,850,522 - hypD b2729 0.32 -0.7
2,850,522 - hypD b2729 0.32 +0.5
2,850,616 - hypD b2729 0.41 -1.5
2,850,624 - hypD b2729 0.42 +0.4
2,850,681 + hypD b2729 0.47 -2.7
2,850,691 - hypD b2729 0.48 -1.4

Or see this region's nucleotide sequence