Experiment: LB Anaerobic with Chloramphenicol 0.000001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfnB and ybdF are separated by 93 nucleotides ybdF and ybdJ are separated by 64 nucleotides ybdJ and ybdK are separated by 65 nucleotides ybdK and sokE are separated by 350 nucleotides sokE and hokE are separated by 43 nucleotides hokE and insL-2 are separated by 76 nucleotides
b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647
nfnB
b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109
ybdF
b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422
ybdJ
b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606
ybdK
b4700: sokE - sRNA at remnant mokE/hokE locus (RefSeq), at 606,957 to 607,015
sokE
b4415: hokE - toxic polypeptide, small (NCBI), at 607,059 to 607,211
hokE
b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400
insL-2
Position (kb) 605
606
607 Strain fitness (log2 ratio) -2
-1
0
1 at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.538 kb on + strand, within nfnB at 604.540 kb on + strand, within nfnB at 604.777 kb on - strand at 604.871 kb on + strand, within ybdF at 604.888 kb on - strand, within ybdF at 604.894 kb on - strand, within ybdF at 605.086 kb on + strand at 605.180 kb on - strand at 605.180 kb on - strand at 605.335 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.472 kb on - strand at 605.498 kb on + strand at 605.549 kb on - strand at 605.683 kb on - strand, within ybdK at 605.699 kb on + strand, within ybdK at 605.713 kb on - strand, within ybdK at 605.713 kb on - strand, within ybdK at 605.725 kb on - strand, within ybdK at 605.754 kb on - strand, within ybdK at 605.862 kb on + strand, within ybdK at 605.862 kb on + strand, within ybdK at 605.877 kb on - strand, within ybdK at 605.918 kb on - strand, within ybdK at 605.953 kb on + strand, within ybdK at 605.966 kb on + strand, within ybdK at 606.060 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.317 kb on - strand, within ybdK at 606.367 kb on - strand, within ybdK at 606.498 kb on + strand at 606.506 kb on - strand at 606.516 kb on - strand at 606.516 kb on - strand at 606.602 kb on + strand at 606.621 kb on - strand at 606.673 kb on + strand at 606.937 kb on + strand at 606.988 kb on + strand, within sokE at 607.102 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.122 kb on + strand, within hokE at 607.185 kb on + strand, within hokE at 607.210 kb on - strand at 607.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Chloramphenicol 0.000001953125 mM remove 604,488 - nfnB b0578 0.76 -1.0 604,488 - nfnB b0578 0.76 +0.3 604,488 - nfnB b0578 0.76 +1.3 604,538 + nfnB b0578 0.83 -0.0 604,540 + nfnB b0578 0.83 -0.3 604,777 - +0.3 604,871 + ybdF b0579 0.35 -0.9 604,888 - ybdF b0579 0.40 +0.3 604,894 - ybdF b0579 0.41 +0.1 605,086 + +0.5 605,180 - -0.9 605,180 - -2.0 605,335 + ybdJ b0580 0.65 +0.6 605,355 + ybdJ b0580 0.73 +0.4 605,355 + ybdJ b0580 0.73 -1.6 605,472 - -0.3 605,498 + +0.1 605,549 - +0.3 605,683 - ybdK b0581 0.17 -0.2 605,699 + ybdK b0581 0.19 +1.0 605,713 - ybdK b0581 0.20 -0.2 605,713 - ybdK b0581 0.20 +0.1 605,725 - ybdK b0581 0.21 +1.2 605,754 - ybdK b0581 0.24 +0.5 605,862 + ybdK b0581 0.33 +0.1 605,862 + ybdK b0581 0.33 +0.9 605,877 - ybdK b0581 0.35 -0.3 605,918 - ybdK b0581 0.38 +0.3 605,953 + ybdK b0581 0.42 -0.1 605,966 + ybdK b0581 0.43 -1.5 606,060 + ybdK b0581 0.51 +0.1 606,095 + ybdK b0581 0.54 -2.3 606,095 + ybdK b0581 0.54 +0.5 606,317 - ybdK b0581 0.74 +1.0 606,367 - ybdK b0581 0.79 +0.2 606,498 + +0.4 606,506 - -0.0 606,516 - -1.1 606,516 - -1.0 606,602 + +1.6 606,621 - -1.0 606,673 + -0.8 606,937 + -0.0 606,988 + sokE b4700 0.53 +0.6 607,102 - hokE b4415 0.28 -2.0 607,105 - hokE b4415 0.30 +0.1 607,105 - hokE b4415 0.30 -0.4 607,122 + hokE b4415 0.41 -1.9 607,185 + hokE b4415 0.82 +0.6 607,210 - +0.3 607,224 + +0.2
Or see this region's nucleotide sequence