Experiment: KB with Harmol Hydrochloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1428 and Psyr_1429 are separated by 20 nucleotides Psyr_1429 and Psyr_1430 overlap by 4 nucleotides Psyr_1430 and Psyr_1431 are separated by 781 nucleotides
Psyr_1428: Psyr_1428 - conserved hypothetical protein, at 1,616,775 to 1,618,142
_1428
Psyr_1429: Psyr_1429 - Protein of unknown function DUF1527, at 1,618,163 to 1,619,101
_1429
Psyr_1430: Psyr_1430 - Protein of unknown function DUF1525, at 1,619,098 to 1,619,571
_1430
Psyr_1431: Psyr_1431 - hypothetical protein, at 1,620,353 to 1,620,643
_1431
Position (kb)
1619
1620 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.143 kb on - strand at 1618.286 kb on + strand, within Psyr_1429 at 1618.286 kb on + strand, within Psyr_1429 at 1618.407 kb on + strand, within Psyr_1429 at 1618.407 kb on + strand, within Psyr_1429 at 1618.408 kb on - strand, within Psyr_1429 at 1618.408 kb on - strand, within Psyr_1429 at 1618.408 kb on - strand, within Psyr_1429 at 1618.408 kb on - strand, within Psyr_1429 at 1618.476 kb on + strand, within Psyr_1429 at 1618.476 kb on + strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.477 kb on - strand, within Psyr_1429 at 1618.495 kb on + strand, within Psyr_1429 at 1618.642 kb on - strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.650 kb on + strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.651 kb on - strand, within Psyr_1429 at 1618.678 kb on - strand, within Psyr_1429 at 1618.700 kb on - strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.759 kb on + strand, within Psyr_1429 at 1618.975 kb on - strand, within Psyr_1429 at 1618.975 kb on - strand, within Psyr_1429 at 1619.198 kb on + strand, within Psyr_1430 at 1619.199 kb on - strand, within Psyr_1430 at 1619.199 kb on - strand, within Psyr_1430 at 1619.282 kb on - strand, within Psyr_1430 at 1619.282 kb on - strand, within Psyr_1430 at 1619.463 kb on + strand, within Psyr_1430 at 1619.500 kb on + strand, within Psyr_1430 at 1619.572 kb on + strand at 1619.573 kb on - strand at 1619.573 kb on - strand at 1619.573 kb on - strand at 1619.580 kb on - strand at 1619.718 kb on + strand at 1619.719 kb on - strand at 1619.746 kb on + strand at 1619.747 kb on - strand at 1619.752 kb on + strand at 1619.752 kb on + strand at 1619.753 kb on - strand at 1619.804 kb on - strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.860 kb on + strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.861 kb on - strand at 1619.913 kb on - strand at 1619.913 kb on - strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.954 kb on + strand at 1619.955 kb on - strand at 1619.955 kb on - strand at 1619.955 kb on - strand at 1619.957 kb on - strand at 1620.049 kb on - strand at 1620.066 kb on - strand at 1620.092 kb on + strand at 1620.093 kb on - strand at 1620.100 kb on + strand at 1620.137 kb on - strand at 1620.177 kb on + strand at 1620.177 kb on + strand at 1620.177 kb on + strand at 1620.177 kb on + strand at 1620.178 kb on - strand at 1620.178 kb on - strand at 1620.178 kb on - strand at 1620.178 kb on - strand at 1620.191 kb on + strand at 1620.191 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.277 kb on + strand at 1620.278 kb on - strand at 1620.278 kb on - strand at 1620.278 kb on - strand at 1620.282 kb on + strand at 1620.299 kb on + strand at 1620.299 kb on + strand at 1620.299 kb on + strand at 1620.299 kb on + strand at 1620.300 kb on - strand at 1620.300 kb on - strand at 1620.300 kb on - strand at 1620.300 kb on - strand at 1620.330 kb on - strand at 1620.330 kb on - strand at 1620.337 kb on + strand at 1620.364 kb on - strand at 1620.364 kb on - strand at 1620.364 kb on - strand at 1620.381 kb on + strand at 1620.391 kb on + strand, within Psyr_1431 at 1620.412 kb on + strand, within Psyr_1431 at 1620.413 kb on - strand, within Psyr_1431 at 1620.447 kb on + strand, within Psyr_1431 at 1620.448 kb on - strand, within Psyr_1431 at 1620.499 kb on - strand, within Psyr_1431 at 1620.556 kb on + strand, within Psyr_1431 at 1620.556 kb on + strand, within Psyr_1431 at 1620.556 kb on + strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.557 kb on - strand, within Psyr_1431 at 1620.559 kb on + strand, within Psyr_1431 at 1620.560 kb on - strand, within Psyr_1431
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol Hydrochloride 10 mM remove 1,618,143 - -0.4 1,618,143 - +0.9 1,618,143 - +0.2 1,618,143 - -0.9 1,618,143 - +0.3 1,618,143 - +0.1 1,618,143 - -0.4 1,618,286 + Psyr_1429 0.13 +0.7 1,618,286 + Psyr_1429 0.13 +0.1 1,618,407 + Psyr_1429 0.26 -0.7 1,618,407 + Psyr_1429 0.26 +2.1 1,618,408 - Psyr_1429 0.26 +0.3 1,618,408 - Psyr_1429 0.26 -0.8 1,618,408 - Psyr_1429 0.26 -0.1 1,618,408 - Psyr_1429 0.26 +0.6 1,618,476 + Psyr_1429 0.33 -0.6 1,618,476 + Psyr_1429 0.33 -0.1 1,618,477 - Psyr_1429 0.33 -1.3 1,618,477 - Psyr_1429 0.33 -1.1 1,618,477 - Psyr_1429 0.33 -1.3 1,618,477 - Psyr_1429 0.33 +0.7 1,618,495 + Psyr_1429 0.35 +0.2 1,618,642 - Psyr_1429 0.51 +0.6 1,618,650 + Psyr_1429 0.52 +0.4 1,618,650 + Psyr_1429 0.52 -1.4 1,618,650 + Psyr_1429 0.52 -0.7 1,618,651 - Psyr_1429 0.52 -2.0 1,618,651 - Psyr_1429 0.52 +0.5 1,618,651 - Psyr_1429 0.52 +1.5 1,618,651 - Psyr_1429 0.52 -0.5 1,618,678 - Psyr_1429 0.55 +1.4 1,618,700 - Psyr_1429 0.57 +0.5 1,618,759 + Psyr_1429 0.63 +0.5 1,618,759 + Psyr_1429 0.63 -0.0 1,618,759 + Psyr_1429 0.63 +1.4 1,618,759 + Psyr_1429 0.63 +0.7 1,618,759 + Psyr_1429 0.63 +0.5 1,618,759 + Psyr_1429 0.63 +1.4 1,618,975 - Psyr_1429 0.86 +0.4 1,618,975 - Psyr_1429 0.86 -0.3 1,619,198 + Psyr_1430 0.21 +0.3 1,619,199 - Psyr_1430 0.21 +0.2 1,619,199 - Psyr_1430 0.21 +0.4 1,619,282 - Psyr_1430 0.39 -2.5 1,619,282 - Psyr_1430 0.39 +0.6 1,619,463 + Psyr_1430 0.77 -0.2 1,619,500 + Psyr_1430 0.85 +1.1 1,619,572 + -0.5 1,619,573 - +0.3 1,619,573 - -0.0 1,619,573 - -0.8 1,619,580 - +0.0 1,619,718 + -0.4 1,619,719 - +0.4 1,619,746 + -0.1 1,619,747 - -0.0 1,619,752 + +1.0 1,619,752 + +0.4 1,619,753 - +0.5 1,619,804 - -0.4 1,619,860 + -1.0 1,619,860 + +0.9 1,619,860 + -0.0 1,619,861 - -0.5 1,619,861 - -0.4 1,619,861 - -1.5 1,619,861 - -0.5 1,619,861 - +1.4 1,619,861 - +0.3 1,619,861 - +0.2 1,619,913 - -0.5 1,619,913 - -1.1 1,619,954 + +0.5 1,619,954 + +0.5 1,619,954 + +0.4 1,619,954 + +0.7 1,619,955 - -0.1 1,619,955 - +0.3 1,619,955 - -1.2 1,619,957 - -1.0 1,620,049 - -0.8 1,620,066 - -0.5 1,620,092 + -0.0 1,620,093 - +0.1 1,620,100 + +0.1 1,620,137 - -0.6 1,620,177 + +0.0 1,620,177 + +0.5 1,620,177 + -0.5 1,620,177 + -0.1 1,620,178 - -0.3 1,620,178 - +0.2 1,620,178 - -1.2 1,620,178 - -1.2 1,620,191 + -0.4 1,620,191 + -0.5 1,620,277 + +0.7 1,620,277 + -0.9 1,620,277 + -0.6 1,620,278 - -0.3 1,620,278 - +0.4 1,620,278 - -0.7 1,620,282 + +0.2 1,620,299 + +0.7 1,620,299 + -0.4 1,620,299 + -0.1 1,620,299 + -0.7 1,620,300 - -1.1 1,620,300 - -1.5 1,620,300 - -0.3 1,620,300 - +0.9 1,620,330 - +0.1 1,620,330 - +0.1 1,620,337 + -0.9 1,620,364 - -0.5 1,620,364 - -0.4 1,620,364 - -0.8 1,620,381 + -2.7 1,620,391 + Psyr_1431 0.13 -0.1 1,620,412 + Psyr_1431 0.20 +0.5 1,620,413 - Psyr_1431 0.21 -1.2 1,620,447 + Psyr_1431 0.32 -0.6 1,620,448 - Psyr_1431 0.33 -3.0 1,620,499 - Psyr_1431 0.50 -0.5 1,620,556 + Psyr_1431 0.70 -0.2 1,620,556 + Psyr_1431 0.70 -0.6 1,620,556 + Psyr_1431 0.70 -0.9 1,620,557 - Psyr_1431 0.70 -1.0 1,620,557 - Psyr_1431 0.70 +2.1 1,620,557 - Psyr_1431 0.70 -0.7 1,620,557 - Psyr_1431 0.70 -0.2 1,620,559 + Psyr_1431 0.71 -0.1 1,620,560 - Psyr_1431 0.71 -0.9
Or see this region's nucleotide sequence