Strain Fitness in Escherichia coli BW25113 around b2688

Experiment: LB Anaerobic with Chloramphenicol 0.00000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntemrB and luxS are separated by 63 nucleotidesluxS and micA are separated by 68 nucleotidesmicA and gshA are separated by 3 nucleotidesgshA and yqaA are separated by 72 nucleotidesyqaA and yqaB overlap by 4 nucleotides b2686: emrB - multidrug efflux system protein (NCBI), at 2,810,638 to 2,812,176 emrB b2687: luxS - S-ribosylhomocysteinase (NCBI), at 2,812,240 to 2,812,755 luxS b4442: micA - sRNA effector of ompA mRNA instability in stationary phase; requires Hfq (RefSeq), at 2,812,824 to 2,812,901 micA b2688: gshA - glutamate--cysteine ligase (NCBI), at 2,812,905 to 2,814,461 gshA b2689: yqaA - conserved inner membrane protein (NCBI), at 2,814,534 to 2,814,962 yqaA b2690: yqaB - predicted hydrolase (NCBI), at 2,814,959 to 2,815,525 yqaB Position (kb) 2812 2813 2814 2815Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2811.906 kb on - strand, within emrBat 2811.983 kb on + strand, within emrBat 2811.983 kb on + strand, within emrBat 2812.033 kb on - strandat 2812.033 kb on - strandat 2812.113 kb on - strandat 2812.113 kb on - strandat 2812.127 kb on + strandat 2812.150 kb on + strandat 2812.150 kb on + strandat 2812.178 kb on - strandat 2812.327 kb on + strand, within luxSat 2812.334 kb on - strand, within luxSat 2812.375 kb on - strand, within luxSat 2812.383 kb on - strand, within luxSat 2812.383 kb on - strand, within luxSat 2812.467 kb on + strand, within luxSat 2812.470 kb on - strand, within luxSat 2812.525 kb on + strand, within luxSat 2812.525 kb on + strand, within luxSat 2812.534 kb on - strand, within luxSat 2812.541 kb on - strand, within luxSat 2812.547 kb on - strand, within luxSat 2812.585 kb on + strand, within luxSat 2812.604 kb on - strand, within luxSat 2812.604 kb on - strand, within luxSat 2812.637 kb on - strand, within luxSat 2812.719 kb on + strandat 2812.819 kb on - strandat 2813.178 kb on - strand, within gshAat 2813.297 kb on + strand, within gshAat 2813.297 kb on + strand, within gshAat 2813.320 kb on - strand, within gshAat 2813.347 kb on - strand, within gshAat 2813.392 kb on + strand, within gshAat 2813.417 kb on + strand, within gshAat 2813.471 kb on - strand, within gshAat 2813.485 kb on - strand, within gshAat 2813.616 kb on + strand, within gshAat 2813.624 kb on - strand, within gshAat 2813.779 kb on + strand, within gshAat 2813.812 kb on - strand, within gshAat 2813.878 kb on - strand, within gshAat 2813.881 kb on - strand, within gshAat 2813.915 kb on - strand, within gshAat 2813.915 kb on - strand, within gshAat 2814.108 kb on - strand, within gshAat 2814.115 kb on + strand, within gshAat 2814.131 kb on - strand, within gshAat 2814.131 kb on - strand, within gshAat 2814.139 kb on + strand, within gshAat 2814.139 kb on + strand, within gshAat 2814.144 kb on + strand, within gshAat 2814.191 kb on + strand, within gshAat 2814.233 kb on + strand, within gshAat 2814.233 kb on + strand, within gshAat 2814.303 kb on + strand, within gshAat 2814.308 kb on + strandat 2814.308 kb on + strandat 2814.316 kb on - strandat 2814.331 kb on - strandat 2814.373 kb on + strandat 2814.552 kb on + strandat 2814.552 kb on + strandat 2814.709 kb on - strand, within yqaAat 2814.709 kb on - strand, within yqaAat 2814.745 kb on - strand, within yqaAat 2814.798 kb on - strand, within yqaAat 2814.900 kb on + strand, within yqaAat 2814.900 kb on + strand, within yqaAat 2814.981 kb on - strandat 2814.981 kb on - strandat 2814.983 kb on + strandat 2814.983 kb on + strandat 2814.991 kb on + strandat 2815.035 kb on + strand, within yqaBat 2815.035 kb on + strand, within yqaBat 2815.051 kb on + strand, within yqaBat 2815.059 kb on + strand, within yqaBat 2815.152 kb on + strand, within yqaBat 2815.153 kb on + strand, within yqaBat 2815.194 kb on - strand, within yqaBat 2815.279 kb on + strand, within yqaBat 2815.381 kb on - strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.460 kb on + strand, within yqaB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chloramphenicol 0.00000390625 mM
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2,811,906 - emrB b2686 0.82 +0.3
2,811,983 + emrB b2686 0.87 +0.5
2,811,983 + emrB b2686 0.87 -0.2
2,812,033 - -0.0
2,812,033 - +1.1
2,812,113 - +0.2
2,812,113 - -1.2
2,812,127 + +0.7
2,812,150 + +0.4
2,812,150 + +0.1
2,812,178 - +1.5
2,812,327 + luxS b2687 0.17 +0.4
2,812,334 - luxS b2687 0.18 -1.3
2,812,375 - luxS b2687 0.26 +0.4
2,812,383 - luxS b2687 0.28 +0.2
2,812,383 - luxS b2687 0.28 +0.6
2,812,467 + luxS b2687 0.44 +0.1
2,812,470 - luxS b2687 0.45 -0.2
2,812,525 + luxS b2687 0.55 -0.0
2,812,525 + luxS b2687 0.55 +1.2
2,812,534 - luxS b2687 0.57 -0.0
2,812,541 - luxS b2687 0.58 -0.3
2,812,547 - luxS b2687 0.59 -1.3
2,812,585 + luxS b2687 0.67 -0.6
2,812,604 - luxS b2687 0.71 +0.1
2,812,604 - luxS b2687 0.71 +0.1
2,812,637 - luxS b2687 0.77 +0.1
2,812,719 + +0.3
2,812,819 - +0.3
2,813,178 - gshA b2688 0.18 +1.0
2,813,297 + gshA b2688 0.25 +2.0
2,813,297 + gshA b2688 0.25 -0.0
2,813,320 - gshA b2688 0.27 +0.7
2,813,347 - gshA b2688 0.28 +1.9
2,813,392 + gshA b2688 0.31 +1.7
2,813,417 + gshA b2688 0.33 +1.5
2,813,471 - gshA b2688 0.36 +2.1
2,813,485 - gshA b2688 0.37 +1.3
2,813,616 + gshA b2688 0.46 +2.3
2,813,624 - gshA b2688 0.46 +0.5
2,813,779 + gshA b2688 0.56 +2.8
2,813,812 - gshA b2688 0.58 +0.7
2,813,878 - gshA b2688 0.62 +2.8
2,813,881 - gshA b2688 0.63 +2.2
2,813,915 - gshA b2688 0.65 +0.7
2,813,915 - gshA b2688 0.65 +1.1
2,814,108 - gshA b2688 0.77 +1.8
2,814,115 + gshA b2688 0.78 +1.5
2,814,131 - gshA b2688 0.79 +1.0
2,814,131 - gshA b2688 0.79 +2.1
2,814,139 + gshA b2688 0.79 -0.5
2,814,139 + gshA b2688 0.79 +0.8
2,814,144 + gshA b2688 0.80 +0.5
2,814,191 + gshA b2688 0.83 +2.5
2,814,233 + gshA b2688 0.85 -0.6
2,814,233 + gshA b2688 0.85 +3.0
2,814,303 + gshA b2688 0.90 +1.6
2,814,308 + +0.4
2,814,308 + -0.0
2,814,316 - +1.0
2,814,331 - +1.7
2,814,373 + +2.3
2,814,552 + +0.9
2,814,552 + +0.6
2,814,709 - yqaA b2689 0.41 -0.8
2,814,709 - yqaA b2689 0.41 -0.1
2,814,745 - yqaA b2689 0.49 -0.6
2,814,798 - yqaA b2689 0.62 -1.0
2,814,900 + yqaA b2689 0.85 +1.0
2,814,900 + yqaA b2689 0.85 -0.5
2,814,981 - +2.0
2,814,981 - -0.6
2,814,983 + -0.5
2,814,983 + -0.4
2,814,991 + +2.5
2,815,035 + yqaB b2690 0.13 +0.5
2,815,035 + yqaB b2690 0.13 +0.5
2,815,051 + yqaB b2690 0.16 +0.4
2,815,059 + yqaB b2690 0.18 -0.7
2,815,152 + yqaB b2690 0.34 +1.2
2,815,153 + yqaB b2690 0.34 +0.2
2,815,194 - yqaB b2690 0.41 -0.9
2,815,279 + yqaB b2690 0.56 -0.8
2,815,381 - yqaB b2690 0.74 -2.7
2,815,430 + yqaB b2690 0.83 -0.1
2,815,430 + yqaB b2690 0.83 -0.3
2,815,430 + yqaB b2690 0.83 +0.5
2,815,460 + yqaB b2690 0.88 +0.4

Or see this region's nucleotide sequence