Experiment: LB Anaerobic with Chloramphenicol 0.00000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfJ and prfH are separated by 16 nucleotides prfH and pepD are separated by 56 nucleotides pepD and gpt are separated by 260 nucleotides gpt and frsA are separated by 91 nucleotides
b0235: ykfJ - hypothetical protein (RefSeq), at 253,479 to 253,685
ykfJ
b0236: prfH - peptide chain release factor 2 (NCBI), at 253,702 to 254,202
prfH
b0237: pepD - aminoacyl-histidine dipeptidase (peptidase D) (NCBI), at 254,259 to 255,716
pepD
b0238: gpt - xanthine phosphoribosyltransferase (NCBI), at 255,977 to 256,435
gpt
b0239: frsA - fermentation/respiration switch protein (NCBI), at 256,527 to 257,771
frsA
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.272 kb on - strand at 253.275 kb on + strand at 253.283 kb on - strand at 253.301 kb on + strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.376 kb on - strand at 253.381 kb on - strand at 253.383 kb on - strand at 253.386 kb on + strand at 253.387 kb on + strand at 253.394 kb on + strand at 253.394 kb on + strand at 253.396 kb on - strand at 253.422 kb on - strand at 253.422 kb on - strand at 253.423 kb on - strand at 253.423 kb on - strand at 253.427 kb on - strand at 253.428 kb on - strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.432 kb on - strand at 253.432 kb on - strand at 253.434 kb on + strand at 253.437 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.444 kb on - strand at 253.450 kb on - strand at 253.454 kb on - strand at 253.491 kb on + strand at 253.504 kb on - strand, within ykfJ at 253.504 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.523 kb on + strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.557 kb on - strand, within ykfJ at 253.569 kb on - strand, within ykfJ at 253.571 kb on - strand, within ykfJ at 253.593 kb on + strand, within ykfJ at 253.603 kb on + strand, within ykfJ at 253.608 kb on - strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.633 kb on - strand, within ykfJ at 253.637 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.681 kb on + strand at 253.727 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.734 kb on - strand at 253.734 kb on - strand at 253.735 kb on - strand at 253.735 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.747 kb on + strand at 253.765 kb on + strand, within prfH at 253.773 kb on + strand, within prfH at 253.805 kb on - strand, within prfH at 253.805 kb on - strand, within prfH at 253.838 kb on - strand, within prfH at 253.891 kb on - strand, within prfH at 254.167 kb on - strand at 254.336 kb on + strand at 254.354 kb on - strand at 254.354 kb on - strand at 254.354 kb on - strand at 254.517 kb on - strand, within pepD at 254.592 kb on - strand, within pepD at 254.593 kb on + strand, within pepD at 254.632 kb on - strand, within pepD at 254.632 kb on - strand, within pepD at 254.693 kb on - strand, within pepD at 254.693 kb on - strand, within pepD at 254.694 kb on - strand, within pepD at 254.694 kb on - strand, within pepD at 254.738 kb on - strand, within pepD at 254.738 kb on - strand, within pepD at 254.781 kb on - strand, within pepD at 254.816 kb on - strand at 255.011 kb on + strand, within pepD at 255.011 kb on + strand, within pepD at 255.037 kb on + strand, within pepD at 255.042 kb on + strand, within pepD at 255.050 kb on - strand, within pepD at 255.128 kb on - strand, within pepD at 255.171 kb on - strand, within pepD at 255.206 kb on - strand, within pepD at 255.206 kb on - strand, within pepD at 255.246 kb on + strand, within pepD at 255.267 kb on + strand, within pepD at 255.267 kb on + strand, within pepD at 255.275 kb on - strand, within pepD at 255.355 kb on - strand, within pepD at 255.371 kb on + strand, within pepD at 255.396 kb on + strand, within pepD at 255.406 kb on - strand, within pepD at 255.426 kb on - strand, within pepD at 255.426 kb on - strand, within pepD at 255.428 kb on + strand, within pepD at 255.428 kb on + strand, within pepD at 255.447 kb on + strand, within pepD at 255.450 kb on + strand, within pepD at 255.455 kb on - strand, within pepD at 255.527 kb on - strand, within pepD at 255.715 kb on - strand at 255.791 kb on + strand at 255.826 kb on - strand at 255.841 kb on - strand at 255.841 kb on - strand at 255.902 kb on + strand at 255.912 kb on - strand at 255.912 kb on - strand at 255.971 kb on + strand at 256.050 kb on + strand, within gpt at 256.050 kb on + strand, within gpt at 256.050 kb on + strand, within gpt at 256.092 kb on + strand, within gpt at 256.250 kb on - strand, within gpt at 256.250 kb on - strand, within gpt at 256.401 kb on - strand at 256.429 kb on - strand at 256.429 kb on - strand at 256.479 kb on - strand at 256.491 kb on + strand at 256.510 kb on + strand at 256.554 kb on - strand at 256.603 kb on + strand at 256.621 kb on - strand at 256.621 kb on - strand at 256.675 kb on + strand, within frsA at 256.705 kb on + strand, within frsA at 256.705 kb on + strand, within frsA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Chloramphenicol 0.00000390625 mM remove 253,272 - +1.0 253,275 + +1.0 253,283 - +1.0 253,301 + +1.0 253,350 - -0.1 253,350 - -0.9 253,350 - -1.3 253,350 - +0.1 253,376 - -0.3 253,381 - -0.6 253,383 - -0.3 253,386 + +1.0 253,387 + +1.0 253,394 + +1.0 253,394 + +1.0 253,396 - +0.2 253,422 - -1.3 253,422 - +2.3 253,423 - +1.0 253,423 - -0.3 253,427 - -0.0 253,428 - -0.7 253,430 + +2.0 253,430 + +1.0 253,430 + -0.6 253,430 + +1.0 253,432 - -1.8 253,432 - +0.4 253,434 + +1.0 253,437 - -0.9 253,438 - +1.0 253,438 - -0.0 253,438 - -0.6 253,438 - -0.6 253,444 - +1.0 253,450 - +1.0 253,454 - +0.4 253,491 + +1.0 253,504 - ykfJ b0235 0.12 +1.5 253,504 - ykfJ b0235 0.12 -0.1 253,520 - ykfJ b0235 0.20 +0.3 253,520 - ykfJ b0235 0.20 +0.3 253,520 - ykfJ b0235 0.20 -0.0 253,523 + ykfJ b0235 0.21 +1.0 253,533 - ykfJ b0235 0.26 -1.6 253,533 - ykfJ b0235 0.26 +1.0 253,533 - ykfJ b0235 0.26 -0.1 253,533 - ykfJ b0235 0.26 +1.0 253,535 + ykfJ b0235 0.27 +1.0 253,535 + ykfJ b0235 0.27 +1.0 253,543 - ykfJ b0235 0.31 +0.4 253,543 - ykfJ b0235 0.31 +0.2 253,543 - ykfJ b0235 0.31 -0.9 253,543 - ykfJ b0235 0.31 +0.6 253,543 - ykfJ b0235 0.31 +0.4 253,543 - ykfJ b0235 0.31 -0.6 253,543 - ykfJ b0235 0.31 +0.1 253,543 - ykfJ b0235 0.31 -0.1 253,557 - ykfJ b0235 0.38 +0.1 253,569 - ykfJ b0235 0.43 -0.1 253,571 - ykfJ b0235 0.44 -0.3 253,593 + ykfJ b0235 0.55 -0.0 253,603 + ykfJ b0235 0.60 -0.0 253,608 - ykfJ b0235 0.62 -0.1 253,617 + ykfJ b0235 0.67 +0.1 253,617 + ykfJ b0235 0.67 -0.9 253,617 + ykfJ b0235 0.67 -1.8 253,617 + ykfJ b0235 0.67 +1.5 253,625 - ykfJ b0235 0.71 -0.0 253,625 - ykfJ b0235 0.71 +1.0 253,625 - ykfJ b0235 0.71 +1.3 253,633 - ykfJ b0235 0.74 -0.0 253,637 - ykfJ b0235 0.76 +0.4 253,642 - ykfJ b0235 0.79 -0.5 253,642 - ykfJ b0235 0.79 +0.4 253,642 - ykfJ b0235 0.79 +0.1 253,681 + +0.4 253,727 + +0.8 253,728 + -0.4 253,728 + +0.2 253,728 + +0.1 253,734 - -0.2 253,734 - +0.7 253,735 - -0.6 253,735 - +0.5 253,736 - +1.0 253,736 - -0.8 253,736 - +0.1 253,747 + -0.5 253,765 + prfH b0236 0.13 +0.4 253,773 + prfH b0236 0.14 +0.2 253,805 - prfH b0236 0.21 +0.4 253,805 - prfH b0236 0.21 -0.3 253,838 - prfH b0236 0.27 -0.3 253,891 - prfH b0236 0.38 +0.2 254,167 - -0.7 254,336 + -1.6 254,354 - +0.6 254,354 - +1.0 254,354 - -1.4 254,517 - pepD b0237 0.18 +1.3 254,592 - pepD b0237 0.23 +0.1 254,593 + pepD b0237 0.23 +0.1 254,632 - pepD b0237 0.26 +0.3 254,632 - pepD b0237 0.26 +0.3 254,693 - pepD b0237 0.30 -0.3 254,693 - pepD b0237 0.30 -0.2 254,694 - pepD b0237 0.30 -0.6 254,694 - pepD b0237 0.30 -0.0 254,738 - pepD b0237 0.33 +0.4 254,738 - pepD b0237 0.33 +0.4 254,781 - pepD b0237 0.36 -0.0 254,816 - +1.0 255,011 + pepD b0237 0.52 -1.4 255,011 + pepD b0237 0.52 +0.4 255,037 + pepD b0237 0.53 -1.6 255,042 + pepD b0237 0.54 -1.1 255,050 - pepD b0237 0.54 +1.0 255,128 - pepD b0237 0.60 -0.8 255,171 - pepD b0237 0.63 -0.0 255,206 - pepD b0237 0.65 -1.0 255,206 - pepD b0237 0.65 -1.0 255,246 + pepD b0237 0.68 -0.6 255,267 + pepD b0237 0.69 -0.8 255,267 + pepD b0237 0.69 -1.7 255,275 - pepD b0237 0.70 -0.0 255,355 - pepD b0237 0.75 +0.8 255,371 + pepD b0237 0.76 -0.7 255,396 + pepD b0237 0.78 -1.1 255,406 - pepD b0237 0.79 -0.9 255,426 - pepD b0237 0.80 -2.8 255,426 - pepD b0237 0.80 -0.5 255,428 + pepD b0237 0.80 +1.0 255,428 + pepD b0237 0.80 +0.7 255,447 + pepD b0237 0.81 +0.4 255,450 + pepD b0237 0.82 -1.6 255,455 - pepD b0237 0.82 -0.4 255,527 - pepD b0237 0.87 -0.7 255,715 - -0.6 255,791 + -2.3 255,826 - +0.3 255,841 - +1.1 255,841 - -0.0 255,902 + +0.7 255,912 - +0.4 255,912 - -0.6 255,971 + +0.6 256,050 + gpt b0238 0.16 -0.7 256,050 + gpt b0238 0.16 +0.1 256,050 + gpt b0238 0.16 -0.2 256,092 + gpt b0238 0.25 +1.2 256,250 - gpt b0238 0.59 +0.4 256,250 - gpt b0238 0.59 +0.5 256,401 - +0.3 256,429 - -0.1 256,429 - +0.8 256,479 - -0.1 256,491 + +0.6 256,510 + +0.4 256,554 - -0.5 256,603 + -1.1 256,621 - -2.2 256,621 - -0.9 256,675 + frsA b0239 0.12 -1.2 256,705 + frsA b0239 0.14 -0.2 256,705 + frsA b0239 0.14 -1.9
Or see this region's nucleotide sequence