Experiment: LB Anaerobic with Benzethonium chloride 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galK and galT are separated by 3 nucleotides galT and galE are separated by 9 nucleotides
b0757: galK - galactokinase GalK (EC 2.7.1.6) <small>(from data)</small>, at 788,054 to 789,202
galK
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
b0759: galE - UDP-galactose-4-epimerase (VIMSS), at 790,262 to 791,278
galE
Position (kb) 789
790
791 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 788.252 kb on + strand, within galK at 788.406 kb on - strand, within galK at 788.406 kb on - strand, within galK at 788.436 kb on - strand, within galK at 788.479 kb on + strand, within galK at 788.479 kb on + strand, within galK at 788.487 kb on - strand, within galK at 788.487 kb on - strand, within galK at 788.518 kb on - strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.600 kb on - strand, within galK at 788.610 kb on + strand, within galK at 788.644 kb on - strand, within galK at 788.648 kb on - strand, within galK at 788.732 kb on + strand, within galK at 788.829 kb on + strand, within galK at 788.832 kb on - strand, within galK at 789.031 kb on + strand, within galK at 789.039 kb on - strand, within galK at 789.050 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.241 kb on - strand at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.264 kb on + strand at 790.264 kb on + strand at 790.298 kb on + strand at 790.385 kb on + strand, within galE at 790.385 kb on + strand, within galE at 790.393 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.508 kb on - strand, within galE at 790.541 kb on + strand, within galE at 790.604 kb on - strand, within galE at 790.604 kb on - strand, within galE at 790.658 kb on + strand, within galE at 790.658 kb on + strand, within galE at 790.668 kb on + strand, within galE at 790.719 kb on + strand, within galE at 790.759 kb on + strand, within galE at 790.950 kb on + strand, within galE at 790.950 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.152 kb on - strand, within galE at 791.174 kb on - strand, within galE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.000015625 mM remove 788,252 + galK b0757 0.17 +0.1 788,406 - galK b0757 0.31 +0.6 788,406 - galK b0757 0.31 +0.6 788,436 - galK b0757 0.33 -0.6 788,479 + galK b0757 0.37 -2.9 788,479 + galK b0757 0.37 +0.1 788,487 - galK b0757 0.38 -0.6 788,487 - galK b0757 0.38 +0.8 788,518 - galK b0757 0.40 -0.4 788,531 + galK b0757 0.42 +0.4 788,531 + galK b0757 0.42 -1.2 788,531 + galK b0757 0.42 +0.2 788,531 + galK b0757 0.42 +0.2 788,600 - galK b0757 0.48 -0.6 788,610 + galK b0757 0.48 +1.0 788,644 - galK b0757 0.51 -0.1 788,648 - galK b0757 0.52 -0.4 788,732 + galK b0757 0.59 -0.7 788,829 + galK b0757 0.67 +0.0 788,832 - galK b0757 0.68 -0.5 789,031 + galK b0757 0.85 -0.4 789,039 - galK b0757 0.86 +0.4 789,050 + galK b0757 0.87 +0.6 789,063 + galK b0757 0.88 +0.8 789,063 + galK b0757 0.88 +0.1 789,241 - -1.0 789,338 - galT b0758 0.13 +0.0 789,338 - galT b0758 0.13 +0.6 789,425 - galT b0758 0.21 +0.5 789,425 - -1.1 789,433 - galT b0758 0.22 -0.1 789,581 - galT b0758 0.36 +0.5 789,661 + galT b0758 0.43 +0.6 789,712 - galT b0758 0.48 +0.5 789,712 - galT b0758 0.48 +0.6 789,774 - galT b0758 0.54 -0.6 789,811 + galT b0758 0.58 +0.3 789,908 + galT b0758 0.67 -0.5 790,161 - -1.1 790,244 - +0.6 790,244 - -0.4 790,244 - -0.7 790,264 + +0.5 790,264 + -2.5 790,298 + -2.8 790,385 + galE b0759 0.12 -2.4 790,385 + galE b0759 0.12 -2.8 790,393 + galE b0759 0.13 -1.6 790,499 + galE b0759 0.23 -2.4 790,499 + galE b0759 0.23 -0.9 790,508 - galE b0759 0.24 -1.7 790,541 + galE b0759 0.27 -3.5 790,604 - galE b0759 0.34 -3.2 790,604 - galE b0759 0.34 -1.7 790,658 + galE b0759 0.39 -1.9 790,658 + galE b0759 0.39 -0.9 790,668 + galE b0759 0.40 -2.8 790,719 + galE b0759 0.45 -2.5 790,759 + galE b0759 0.49 -4.5 790,950 + galE b0759 0.68 -1.7 790,950 + galE b0759 0.68 -1.4 791,027 + galE b0759 0.75 -2.9 791,027 + galE b0759 0.75 -3.3 791,152 - galE b0759 0.88 -3.5 791,174 - galE b0759 0.90 -3.4
Or see this region's nucleotide sequence