Strain Fitness in Escherichia coli BW25113 around b2519

Experiment: LB Anaerobic with Zinc Pyrithione 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfgB and ndk are separated by 149 nucleotidesndk and pbpC are separated by 148 nucleotidespbpC and yfhM are separated by 0 nucleotides b2517: yfgB - predicted enzyme (NCBI), at 2,641,151 to 2,642,305 yfgB b2518: ndk - nucleoside diphosphate kinase (NCBI), at 2,642,455 to 2,642,886 ndk b2519: pbpC - fused transglycosylase/transpeptidase (NCBI), at 2,643,035 to 2,645,347 pbpC b2520: yfhM - hypothetical protein (NCBI), at 2,645,348 to 2,650,309 yfhM Position (kb) 2643 2644 2645 2646Strain fitness (log2 ratio) -1 0 1 2 3at 2642.153 kb on - strand, within yfgBat 2642.153 kb on - strand, within yfgBat 2642.304 kb on - strandat 2642.392 kb on - strandat 2642.392 kb on - strandat 2642.509 kb on - strand, within ndkat 2642.509 kb on - strand, within ndkat 2642.590 kb on - strand, within ndkat 2642.592 kb on + strand, within ndkat 2642.684 kb on - strandat 2642.740 kb on - strand, within ndkat 2642.740 kb on - strand, within ndkat 2642.809 kb on + strand, within ndkat 2642.937 kb on + strandat 2643.036 kb on - strandat 2643.081 kb on + strandat 2643.131 kb on - strandat 2643.171 kb on - strandat 2643.235 kb on - strandat 2643.235 kb on - strandat 2643.567 kb on + strand, within pbpCat 2643.567 kb on + strand, within pbpCat 2643.567 kb on + strand, within pbpCat 2643.575 kb on - strand, within pbpCat 2643.627 kb on + strand, within pbpCat 2643.645 kb on - strand, within pbpCat 2643.690 kb on + strand, within pbpCat 2644.120 kb on + strand, within pbpCat 2644.127 kb on + strand, within pbpCat 2644.128 kb on - strand, within pbpCat 2644.139 kb on - strand, within pbpCat 2644.251 kb on + strand, within pbpCat 2644.251 kb on + strand, within pbpCat 2644.253 kb on + strand, within pbpCat 2644.253 kb on + strand, within pbpCat 2644.379 kb on + strand, within pbpCat 2644.626 kb on - strand, within pbpCat 2644.651 kb on + strand, within pbpCat 2644.752 kb on + strand, within pbpCat 2644.781 kb on + strand, within pbpCat 2644.781 kb on + strand, within pbpCat 2644.928 kb on - strand, within pbpCat 2644.993 kb on - strand, within pbpCat 2645.046 kb on - strand, within pbpCat 2645.113 kb on + strand, within pbpCat 2645.218 kb on + strandat 2645.250 kb on - strandat 2645.255 kb on + strandat 2645.255 kb on + strandat 2645.331 kb on + strandat 2645.331 kb on + strandat 2645.403 kb on + strandat 2645.411 kb on - strandat 2645.484 kb on + strandat 2645.576 kb on + strandat 2645.712 kb on - strandat 2645.715 kb on - strandat 2645.784 kb on - strandat 2645.784 kb on - strandat 2645.885 kb on - strand, within yfhMat 2645.940 kb on + strand, within yfhMat 2645.945 kb on + strand, within yfhMat 2645.945 kb on + strand, within yfhMat 2645.955 kb on - strand, within yfhMat 2646.087 kb on + strand, within yfhMat 2646.101 kb on + strand, within yfhMat 2646.169 kb on - strand, within yfhMat 2646.241 kb on + strand, within yfhMat 2646.241 kb on + strand, within yfhMat 2646.251 kb on - strand, within yfhM

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Zinc Pyrithione 0.0000025 mM
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2,642,153 - yfgB b2517 0.87 -1.6
2,642,153 - yfgB b2517 0.87 -0.5
2,642,304 - +1.1
2,642,392 - -0.3
2,642,392 - +0.6
2,642,509 - ndk b2518 0.12 -1.5
2,642,509 - ndk b2518 0.12 -1.3
2,642,590 - ndk b2518 0.31 -0.0
2,642,592 + ndk b2518 0.32 +1.0
2,642,684 - -0.5
2,642,740 - ndk b2518 0.66 -0.6
2,642,740 - ndk b2518 0.66 -0.2
2,642,809 + ndk b2518 0.82 -0.5
2,642,937 + -1.0
2,643,036 - +1.3
2,643,081 + +0.2
2,643,131 - +0.4
2,643,171 - +0.7
2,643,235 - -0.2
2,643,235 - +0.3
2,643,567 + pbpC b2519 0.23 +0.2
2,643,567 + pbpC b2519 0.23 +0.2
2,643,567 + pbpC b2519 0.23 +0.4
2,643,575 - pbpC b2519 0.23 -0.4
2,643,627 + pbpC b2519 0.26 +3.6
2,643,645 - pbpC b2519 0.26 +0.6
2,643,690 + pbpC b2519 0.28 +0.8
2,644,120 + pbpC b2519 0.47 +0.1
2,644,127 + pbpC b2519 0.47 +0.7
2,644,128 - pbpC b2519 0.47 -0.2
2,644,139 - pbpC b2519 0.48 -0.3
2,644,251 + pbpC b2519 0.53 +0.9
2,644,251 + pbpC b2519 0.53 +0.5
2,644,253 + pbpC b2519 0.53 +0.9
2,644,253 + pbpC b2519 0.53 +0.1
2,644,379 + pbpC b2519 0.58 +1.5
2,644,626 - pbpC b2519 0.69 +1.1
2,644,651 + pbpC b2519 0.70 +0.4
2,644,752 + pbpC b2519 0.74 -0.4
2,644,781 + pbpC b2519 0.75 +0.8
2,644,781 + pbpC b2519 0.75 -0.0
2,644,928 - pbpC b2519 0.82 -0.1
2,644,993 - pbpC b2519 0.85 +0.5
2,645,046 - pbpC b2519 0.87 -0.4
2,645,113 + pbpC b2519 0.90 +0.2
2,645,218 + +1.0
2,645,250 - -0.1
2,645,255 + -0.6
2,645,255 + +0.1
2,645,331 + +0.1
2,645,331 + +0.2
2,645,403 + +0.3
2,645,411 - -0.2
2,645,484 + +0.1
2,645,576 + -0.8
2,645,712 - -0.4
2,645,715 - +0.8
2,645,784 - -0.8
2,645,784 - -0.4
2,645,885 - yfhM b2520 0.11 -1.5
2,645,940 + yfhM b2520 0.12 -0.8
2,645,945 + yfhM b2520 0.12 +0.5
2,645,945 + yfhM b2520 0.12 +0.1
2,645,955 - yfhM b2520 0.12 -0.6
2,646,087 + yfhM b2520 0.15 +0.5
2,646,101 + yfhM b2520 0.15 +1.1
2,646,169 - yfhM b2520 0.17 -1.3
2,646,241 + yfhM b2520 0.18 -0.5
2,646,241 + yfhM b2520 0.18 -0.6
2,646,251 - yfhM b2520 0.18 +0.0

Or see this region's nucleotide sequence