Experiment: LB Anaerobic with Zinc Pyrithione 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliY and fliZ are separated by 87 nucleotides fliZ and fliA are separated by 45 nucleotides fliA and fliC are separated by 320 nucleotides
b1920: fliY - cystine transporter subunit (NCBI), at 1,997,609 to 1,998,409
fliY
b1921: fliZ - orf, hypothetical protein (VIMSS), at 1,998,497 to 1,999,048
fliZ
b1922: fliA - flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS), at 1,999,094 to 1,999,813
fliA
b1923: fliC - flagellin (NCBI), at 2,000,134 to 2,001,630
fliC
Position (kb)
1999
2000 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1998.266 kb on + strand, within fliY at 1998.375 kb on + strand at 1998.375 kb on + strand at 1998.387 kb on - strand at 1998.549 kb on - strand at 1998.568 kb on + strand, within fliZ at 1998.568 kb on - strand, within fliZ at 1998.616 kb on - strand, within fliZ at 1998.981 kb on + strand, within fliZ at 1998.981 kb on + strand, within fliZ at 1999.139 kb on + strand at 1999.145 kb on - strand at 1999.145 kb on - strand at 1999.177 kb on - strand, within fliA at 1999.209 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.302 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.508 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.541 kb on + strand, within fliA at 1999.595 kb on - strand, within fliA at 1999.600 kb on - strand, within fliA at 1999.615 kb on + strand, within fliA at 1999.655 kb on + strand, within fliA at 1999.800 kb on - strand at 1999.832 kb on + strand at 1999.903 kb on - strand at 1999.969 kb on + strand at 1999.969 kb on + strand at 2000.236 kb on - strand at 2000.439 kb on - strand, within fliC at 2000.523 kb on + strand, within fliC at 2000.591 kb on + strand, within fliC at 2000.591 kb on + strand, within fliC at 2000.766 kb on - strand, within fliC at 2000.772 kb on + strand, within fliC at 2000.772 kb on + strand, within fliC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Zinc Pyrithione 0.0000025 mM remove 1,998,266 + fliY b1920 0.82 -0.1 1,998,375 + -0.5 1,998,375 + -0.5 1,998,387 - -0.1 1,998,549 - -0.3 1,998,568 + fliZ b1921 0.13 -1.1 1,998,568 - fliZ b1921 0.13 -0.3 1,998,616 - fliZ b1921 0.22 -0.5 1,998,981 + fliZ b1921 0.88 -2.3 1,998,981 + fliZ b1921 0.88 -0.4 1,999,139 + -0.9 1,999,145 - -0.5 1,999,145 - -1.1 1,999,177 - fliA b1922 0.12 -3.6 1,999,209 - fliA b1922 0.16 -0.2 1,999,292 - fliA b1922 0.28 -0.5 1,999,292 - fliA b1922 0.28 -1.9 1,999,302 - fliA b1922 0.29 -1.2 1,999,504 - fliA b1922 0.57 -1.3 1,999,504 - fliA b1922 0.57 +0.1 1,999,508 - fliA b1922 0.57 +2.1 1,999,509 - fliA b1922 0.58 -1.5 1,999,509 - fliA b1922 0.58 -1.1 1,999,541 + fliA b1922 0.62 -1.4 1,999,595 - fliA b1922 0.70 -0.7 1,999,600 - fliA b1922 0.70 -0.7 1,999,615 + fliA b1922 0.72 -0.7 1,999,655 + fliA b1922 0.78 -1.5 1,999,800 - -1.6 1,999,832 + +0.7 1,999,903 - -1.1 1,999,969 + -0.4 1,999,969 + -0.0 2,000,236 - -0.7 2,000,439 - fliC b1923 0.20 -1.1 2,000,523 + fliC b1923 0.26 -0.2 2,000,591 + fliC b1923 0.31 -0.3 2,000,591 + fliC b1923 0.31 -0.1 2,000,766 - fliC b1923 0.42 -1.6 2,000,772 + fliC b1923 0.43 -1.5 2,000,772 + fliC b1923 0.43 -2.5
Or see this region's nucleotide sequence