Experiment: LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemX and hemD are separated by 21 nucleotides hemD and hemC overlap by 4 nucleotides
b3803: hemX - predicted uroporphyrinogen III methylase (NCBI), at 3,985,908 to 3,987,089
hemX
b3804: hemD - uroporphyrinogen-III synthetase (NCBI), at 3,987,111 to 3,987,851
hemD
b3805: hemC - porphobilinogen deaminase (NCBI), at 3,987,848 to 3,988,789
hemC
Position (kb)
3987
3988 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3986.129 kb on + strand, within hemX at 3986.139 kb on + strand, within hemX at 3986.139 kb on + strand, within hemX at 3986.149 kb on - strand, within hemX at 3986.150 kb on + strand, within hemX at 3986.151 kb on + strand, within hemX at 3986.151 kb on + strand, within hemX at 3986.168 kb on + strand, within hemX at 3986.168 kb on + strand, within hemX at 3986.180 kb on + strand, within hemX at 3986.180 kb on + strand, within hemX at 3986.207 kb on + strand, within hemX at 3986.207 kb on + strand, within hemX at 3986.215 kb on - strand, within hemX at 3986.215 kb on - strand, within hemX at 3986.280 kb on - strand, within hemX at 3986.356 kb on + strand, within hemX at 3986.356 kb on + strand, within hemX at 3986.385 kb on - strand, within hemX at 3986.387 kb on + strand, within hemX at 3986.432 kb on - strand, within hemX at 3986.491 kb on + strand, within hemX at 3986.491 kb on + strand, within hemX at 3986.498 kb on - strand, within hemX at 3986.510 kb on + strand, within hemX at 3986.510 kb on + strand, within hemX at 3986.512 kb on + strand, within hemX at 3986.536 kb on + strand, within hemX at 3986.536 kb on + strand, within hemX at 3986.539 kb on - strand, within hemX at 3986.564 kb on - strand, within hemX at 3986.571 kb on + strand, within hemX at 3986.571 kb on + strand, within hemX at 3986.601 kb on + strand, within hemX at 3986.609 kb on + strand, within hemX at 3986.618 kb on - strand, within hemX at 3986.620 kb on + strand, within hemX at 3986.623 kb on + strand, within hemX at 3986.627 kb on + strand, within hemX at 3986.627 kb on + strand, within hemX at 3986.628 kb on + strand, within hemX at 3986.638 kb on + strand, within hemX at 3986.638 kb on + strand, within hemX at 3986.812 kb on - strand, within hemX at 3986.846 kb on - strand, within hemX at 3986.897 kb on + strand, within hemX at 3986.897 kb on + strand, within hemX at 3986.922 kb on + strand, within hemX at 3986.948 kb on + strand, within hemX at 3986.964 kb on - strand, within hemX at 3986.964 kb on - strand, within hemX at 3987.023 kb on + strand at 3987.023 kb on + strand at 3987.027 kb on - strand at 3987.027 kb on - strand at 3987.031 kb on + strand at 3987.033 kb on + strand at 3987.048 kb on + strand at 3987.048 kb on - strand at 3987.051 kb on - strand at 3987.059 kb on - strand at 3987.101 kb on + strand at 3987.114 kb on + strand at 3988.789 kb on - strand at 3988.800 kb on - strand at 3988.800 kb on - strand at 3988.830 kb on - strand at 3988.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM remove 3,986,129 + hemX b3803 0.19 -2.7 3,986,139 + hemX b3803 0.20 +0.0 3,986,139 + hemX b3803 0.20 -1.3 3,986,149 - hemX b3803 0.20 -2.9 3,986,150 + hemX b3803 0.20 -4.4 3,986,151 + hemX b3803 0.21 -2.5 3,986,151 + hemX b3803 0.21 -3.5 3,986,168 + hemX b3803 0.22 -0.3 3,986,168 + hemX b3803 0.22 +0.8 3,986,180 + hemX b3803 0.23 -0.5 3,986,180 + hemX b3803 0.23 +0.0 3,986,207 + hemX b3803 0.25 -4.5 3,986,207 + hemX b3803 0.25 -2.4 3,986,215 - hemX b3803 0.26 +1.6 3,986,215 - hemX b3803 0.26 -1.5 3,986,280 - hemX b3803 0.31 +0.9 3,986,356 + hemX b3803 0.38 -4.0 3,986,356 + hemX b3803 0.38 -1.0 3,986,385 - hemX b3803 0.40 -1.0 3,986,387 + hemX b3803 0.41 -1.3 3,986,432 - hemX b3803 0.44 -1.1 3,986,491 + hemX b3803 0.49 -0.5 3,986,491 + hemX b3803 0.49 +0.6 3,986,498 - hemX b3803 0.50 -1.5 3,986,510 + hemX b3803 0.51 -1.3 3,986,510 + hemX b3803 0.51 -2.3 3,986,512 + hemX b3803 0.51 -2.3 3,986,536 + hemX b3803 0.53 -1.2 3,986,536 + hemX b3803 0.53 -1.3 3,986,539 - hemX b3803 0.53 -3.5 3,986,564 - hemX b3803 0.55 -1.0 3,986,571 + hemX b3803 0.56 -1.5 3,986,571 + hemX b3803 0.56 -0.8 3,986,601 + hemX b3803 0.59 -2.9 3,986,609 + hemX b3803 0.59 -2.8 3,986,618 - hemX b3803 0.60 -1.2 3,986,620 + hemX b3803 0.60 -2.3 3,986,623 + hemX b3803 0.60 -1.9 3,986,627 + hemX b3803 0.61 -2.7 3,986,627 + hemX b3803 0.61 +0.6 3,986,628 + hemX b3803 0.61 -2.3 3,986,638 + hemX b3803 0.62 -1.3 3,986,638 + hemX b3803 0.62 -1.0 3,986,812 - hemX b3803 0.76 +0.6 3,986,846 - hemX b3803 0.79 -2.0 3,986,897 + hemX b3803 0.84 -2.5 3,986,897 + hemX b3803 0.84 -2.1 3,986,922 + hemX b3803 0.86 -2.3 3,986,948 + hemX b3803 0.88 -1.0 3,986,964 - hemX b3803 0.89 -0.1 3,986,964 - hemX b3803 0.89 +0.5 3,987,023 + -2.3 3,987,023 + -1.1 3,987,027 - -0.8 3,987,027 - +0.1 3,987,031 + -0.7 3,987,033 + -2.3 3,987,048 + -2.3 3,987,048 - -0.2 3,987,051 - -0.4 3,987,059 - -2.1 3,987,101 + +0.2 3,987,114 + -0.5 3,988,789 - +1.3 3,988,800 - +0.4 3,988,800 - -0.2 3,988,830 - -0.7 3,988,830 - +0.7
Or see this region's nucleotide sequence