Strain Fitness in Escherichia coli BW25113 around b2135

Experiment: LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpbpG and yohC are separated by 173 nucleotidesyohC and yohD are separated by 169 nucleotidesyohD and yohF are separated by 129 nucleotides b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892 pbpG b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653 yohC b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401 yohD b2137: yohF - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,224,531 to 2,225,292 yohF Position (kb) 2223 2224Strain fitness (log2 ratio) -2 -1 0 1at 2222.154 kb on + strand, within pbpGat 2222.163 kb on + strand, within pbpGat 2222.171 kb on - strand, within pbpGat 2222.183 kb on + strand, within pbpGat 2222.254 kb on - strand, within pbpGat 2222.306 kb on + strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.602 kb on - strand, within pbpGat 2222.804 kb on - strandat 2222.806 kb on - strandat 2222.976 kb on - strandat 2223.193 kb on - strand, within yohCat 2223.323 kb on - strand, within yohCat 2223.457 kb on - strand, within yohCat 2223.476 kb on - strand, within yohCat 2223.531 kb on + strand, within yohCat 2223.573 kb on - strand, within yohCat 2223.647 kb on + strandat 2223.831 kb on + strandat 2223.879 kb on + strandat 2224.007 kb on - strand, within yohDat 2224.171 kb on - strand, within yohDat 2224.249 kb on - strand, within yohDat 2224.348 kb on - strandat 2224.358 kb on + strandat 2224.417 kb on - strandat 2224.529 kb on - strandat 2224.541 kb on - strandat 2224.578 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
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2,222,154 + pbpG b2134 0.21 +0.7
2,222,163 + pbpG b2134 0.22 +0.6
2,222,171 - pbpG b2134 0.23 +0.5
2,222,183 + pbpG b2134 0.24 -2.1
2,222,254 - pbpG b2134 0.32 +1.0
2,222,306 + pbpG b2134 0.37 -0.1
2,222,314 - pbpG b2134 0.38 -0.2
2,222,314 - pbpG b2134 0.38 +0.0
2,222,314 - pbpG b2134 0.38 -0.4
2,222,314 - pbpG b2134 0.38 +0.6
2,222,552 - pbpG b2134 0.63 +0.7
2,222,552 - pbpG b2134 0.63 +0.3
2,222,602 - pbpG b2134 0.69 -0.3
2,222,804 - +1.6
2,222,806 - +0.6
2,222,976 - +0.2
2,223,193 - yohC b2135 0.22 -1.1
2,223,323 - yohC b2135 0.44 -0.2
2,223,457 - yohC b2135 0.66 +0.7
2,223,476 - yohC b2135 0.70 +0.7
2,223,531 + yohC b2135 0.79 -0.2
2,223,573 - yohC b2135 0.86 -1.8
2,223,647 + -1.4
2,223,831 + +0.4
2,223,879 + +0.3
2,224,007 - yohD b2136 0.32 -0.2
2,224,171 - yohD b2136 0.60 +1.1
2,224,249 - yohD b2136 0.74 -0.5
2,224,348 - +0.6
2,224,358 + -0.4
2,224,417 - -2.0
2,224,529 - +1.2
2,224,541 - +0.3
2,224,578 + +0.8

Or see this region's nucleotide sequence