Experiment: KB with Usnic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0246 and Psyr_0247 are separated by 43 nucleotides Psyr_0247 and Psyr_0248 are separated by 69 nucleotides Psyr_0248 and Psyr_0249 are separated by 141 nucleotides Psyr_0249 and Psyr_0250 are separated by 110 nucleotides Psyr_0250 and Psyr_0251 are separated by 89 nucleotides
Psyr_0246: Psyr_0246 - aminomethyltransferase, at 259,665 to 260,747
_0246
Psyr_0247: Psyr_0247 - glycine cleavage system H protein, at 260,791 to 261,177
_0247
Psyr_0248: Psyr_0248 - conserved hypothetical protein, at 261,247 to 261,567
_0248
Psyr_0249: Psyr_0249 - type II secretion system protein E:General secretory system II, protein E, N-terminal, at 261,709 to 263,493
_0249
Psyr_0250: Psyr_0250 - conserved hypothetical protein, at 263,604 to 263,987
_0250
Psyr_0251: Psyr_0251 - transcriptional regulator, AsnC family, at 264,077 to 264,547
_0251
Position (kb) 261
262
263
264 Strain fitness (log2 ratio) -2
-1
0
1
2 at 260.746 kb on - strand at 260.746 kb on - strand at 260.746 kb on - strand at 260.746 kb on - strand at 260.746 kb on - strand at 260.750 kb on + strand at 260.751 kb on - strand at 260.751 kb on - strand at 260.788 kb on + strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.800 kb on - strand at 260.800 kb on - strand at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.079 kb on + strand, within Psyr_0247 at 261.154 kb on + strand at 261.155 kb on - strand at 261.155 kb on - strand at 261.180 kb on + strand at 261.223 kb on - strand at 261.249 kb on - strand at 261.274 kb on - strand at 261.450 kb on - strand, within Psyr_0248 at 261.450 kb on - strand, within Psyr_0248 at 261.486 kb on + strand, within Psyr_0248 at 261.539 kb on - strand at 261.575 kb on + strand at 261.576 kb on - strand at 261.576 kb on - strand at 261.576 kb on - strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.612 kb on - strand at 261.612 kb on - strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.757 kb on - strand at 261.757 kb on - strand at 261.757 kb on - strand at 262.106 kb on + strand, within Psyr_0249 at 262.106 kb on + strand, within Psyr_0249 at 262.107 kb on - strand, within Psyr_0249 at 262.415 kb on + strand, within Psyr_0249 at 262.415 kb on + strand, within Psyr_0249 at 262.415 kb on + strand, within Psyr_0249 at 262.416 kb on - strand, within Psyr_0249 at 262.416 kb on - strand, within Psyr_0249 at 262.416 kb on - strand, within Psyr_0249 at 262.461 kb on + strand, within Psyr_0249 at 262.462 kb on - strand, within Psyr_0249 at 262.546 kb on + strand, within Psyr_0249 at 262.547 kb on - strand, within Psyr_0249 at 262.547 kb on - strand, within Psyr_0249 at 262.547 kb on - strand, within Psyr_0249 at 262.845 kb on - strand, within Psyr_0249 at 263.102 kb on + strand, within Psyr_0249 at 263.103 kb on - strand, within Psyr_0249 at 263.103 kb on - strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.237 kb on + strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.238 kb on - strand, within Psyr_0249 at 263.414 kb on + strand at 263.414 kb on + strand at 263.415 kb on - strand at 263.538 kb on - strand at 263.538 kb on - strand at 263.568 kb on + strand at 263.569 kb on - strand at 263.569 kb on - strand at 263.569 kb on - strand at 263.569 kb on - strand at 263.569 kb on - strand at 263.803 kb on + strand, within Psyr_0250 at 263.804 kb on - strand, within Psyr_0250 at 263.804 kb on - strand, within Psyr_0250 at 263.804 kb on - strand, within Psyr_0250 at 263.906 kb on + strand, within Psyr_0250 at 263.907 kb on - strand, within Psyr_0250 at 263.907 kb on - strand, within Psyr_0250 at 263.957 kb on + strand at 263.957 kb on + strand at 263.957 kb on + strand at 264.033 kb on - strand at 264.059 kb on + strand at 264.060 kb on - strand at 264.072 kb on + strand at 264.077 kb on - strand at 264.098 kb on + strand at 264.099 kb on - strand at 264.099 kb on - strand at 264.282 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.374 kb on + strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251 at 264.375 kb on - strand, within Psyr_0251
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Usnic Acid 10 mM remove 260,746 - +0.7 260,746 - -0.3 260,746 - -0.4 260,746 - -0.5 260,746 - +0.3 260,750 + +2.0 260,751 - +0.4 260,751 - +0.3 260,788 + +0.3 260,789 - +1.2 260,789 - +0.9 260,789 - -0.8 260,789 - +0.7 260,789 - -0.7 260,800 - +0.5 260,800 - -0.5 261,001 + Psyr_0247 0.54 +0.3 261,001 + Psyr_0247 0.54 -0.1 261,001 + Psyr_0247 0.54 -0.3 261,002 - Psyr_0247 0.55 +0.6 261,002 - Psyr_0247 0.55 +0.3 261,002 - Psyr_0247 0.55 +0.2 261,079 + Psyr_0247 0.74 -0.1 261,154 + -0.5 261,155 - -0.7 261,155 - -0.7 261,180 + -1.3 261,223 - -0.5 261,249 - +0.1 261,274 - +1.1 261,450 - Psyr_0248 0.63 +0.9 261,450 - Psyr_0248 0.63 +1.2 261,486 + Psyr_0248 0.74 +0.2 261,539 - -1.3 261,575 + -0.7 261,576 - -0.0 261,576 - -0.9 261,576 - +0.4 261,611 + +0.5 261,611 + -0.4 261,611 + +0.3 261,611 + -0.5 261,611 + +0.4 261,611 + +1.3 261,611 + +0.5 261,611 + -0.1 261,611 + -0.6 261,612 - +0.3 261,612 - +0.9 261,624 + -0.4 261,624 + -0.1 261,624 + +0.7 261,624 + -1.0 261,624 + +0.3 261,624 + +0.1 261,624 + +0.3 261,624 + +1.3 261,624 + -0.9 261,625 - +1.3 261,625 - -0.6 261,625 - +0.4 261,625 - +0.1 261,625 - -0.3 261,625 - +0.6 261,625 - -0.2 261,625 - -0.1 261,757 - +1.1 261,757 - +1.0 261,757 - +0.1 262,106 + Psyr_0249 0.22 -0.2 262,106 + Psyr_0249 0.22 +0.5 262,107 - Psyr_0249 0.22 +2.3 262,415 + Psyr_0249 0.40 -0.6 262,415 + Psyr_0249 0.40 -0.3 262,415 + Psyr_0249 0.40 -1.3 262,416 - Psyr_0249 0.40 +0.7 262,416 - Psyr_0249 0.40 -0.7 262,416 - Psyr_0249 0.40 +1.1 262,461 + Psyr_0249 0.42 +0.5 262,462 - Psyr_0249 0.42 -0.7 262,546 + Psyr_0249 0.47 -0.0 262,547 - Psyr_0249 0.47 -0.2 262,547 - Psyr_0249 0.47 +0.1 262,547 - Psyr_0249 0.47 +0.4 262,845 - Psyr_0249 0.64 +0.4 263,102 + Psyr_0249 0.78 -0.6 263,103 - Psyr_0249 0.78 -1.1 263,103 - Psyr_0249 0.78 +0.3 263,237 + Psyr_0249 0.86 +0.4 263,237 + Psyr_0249 0.86 -0.2 263,237 + Psyr_0249 0.86 +0.0 263,237 + Psyr_0249 0.86 -0.3 263,237 + Psyr_0249 0.86 -1.0 263,237 + Psyr_0249 0.86 +0.8 263,237 + Psyr_0249 0.86 +0.9 263,237 + Psyr_0249 0.86 +1.1 263,237 + Psyr_0249 0.86 +0.2 263,237 + Psyr_0249 0.86 -0.2 263,237 + Psyr_0249 0.86 -0.1 263,237 + Psyr_0249 0.86 -1.0 263,237 + Psyr_0249 0.86 +2.3 263,237 + Psyr_0249 0.86 -0.1 263,237 + Psyr_0249 0.86 +1.3 263,237 + Psyr_0249 0.86 -0.5 263,237 + Psyr_0249 0.86 +0.2 263,238 - Psyr_0249 0.86 +0.8 263,238 - Psyr_0249 0.86 -0.1 263,238 - Psyr_0249 0.86 -0.2 263,238 - Psyr_0249 0.86 +0.6 263,238 - Psyr_0249 0.86 +0.1 263,238 - Psyr_0249 0.86 -0.7 263,238 - Psyr_0249 0.86 -0.0 263,238 - Psyr_0249 0.86 -0.5 263,238 - Psyr_0249 0.86 -0.7 263,238 - Psyr_0249 0.86 -0.0 263,414 + -0.2 263,414 + -0.1 263,415 - -1.3 263,538 - +0.3 263,538 - +0.5 263,568 + +1.5 263,569 - +0.1 263,569 - +0.6 263,569 - -0.4 263,569 - +0.9 263,569 - -0.7 263,803 + Psyr_0250 0.52 -0.2 263,804 - Psyr_0250 0.52 +0.1 263,804 - Psyr_0250 0.52 +0.9 263,804 - Psyr_0250 0.52 -0.2 263,906 + Psyr_0250 0.79 +0.7 263,907 - Psyr_0250 0.79 +0.2 263,907 - Psyr_0250 0.79 -1.0 263,957 + +0.2 263,957 + +0.4 263,957 + -0.4 264,033 - -0.3 264,059 + +0.6 264,060 - -0.6 264,072 + -0.5 264,077 - -0.3 264,098 + -0.7 264,099 - -1.0 264,099 - +1.3 264,282 + Psyr_0251 0.44 -0.3 264,374 + Psyr_0251 0.63 -0.2 264,374 + Psyr_0251 0.63 -0.8 264,374 + Psyr_0251 0.63 -0.4 264,374 + Psyr_0251 0.63 -1.4 264,374 + Psyr_0251 0.63 -0.0 264,374 + Psyr_0251 0.63 +0.6 264,374 + Psyr_0251 0.63 -0.6 264,374 + Psyr_0251 0.63 -0.6 264,374 + Psyr_0251 0.63 -0.0 264,375 - Psyr_0251 0.63 -2.5 264,375 - Psyr_0251 0.63 +0.3 264,375 - Psyr_0251 0.63 +0.2 264,375 - Psyr_0251 0.63 -0.6 264,375 - Psyr_0251 0.63 -0.5 264,375 - Psyr_0251 0.63 -0.2 264,375 - Psyr_0251 0.63 -0.0 264,375 - Psyr_0251 0.63 +1.3 264,375 - Psyr_0251 0.63 +0.3 264,375 - Psyr_0251 0.63 +0.0
Or see this region's nucleotide sequence