Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_0729

Experiment: KB with Gossypol-Acetic Acid Complex 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_RNA9 and Psyr_RNA10 are separated by 29 nucleotidesPsyr_RNA10 and Psyr_RNA11 are separated by 5 nucleotidesPsyr_RNA11 and Psyr_RNA12 are separated by 95 nucleotidesPsyr_RNA12 and Psyr_0729 are separated by 326 nucleotidesPsyr_0729 and Psyr_0730 overlap by 59 nucleotidesPsyr_0730 and Psyr_0731 overlap by 4 nucleotides Psyr_RNA9: Psyr_RNA9 - tRNA-Asn, at 835,185 to 835,260 _RNA9 Psyr_RNA10: Psyr_RNA10 - tRNA-Pro, at 835,290 to 835,366 _RNA10 Psyr_RNA11: Psyr_RNA11 - tRNA-Lys, at 835,372 to 835,447 _RNA11 Psyr_RNA12: Psyr_RNA12 - tRNA-Pro, at 835,543 to 835,619 _RNA12 Psyr_0729: Psyr_0729 - CDS, at 835,946 to 837,109 _0729 Psyr_0730: Psyr_0730 - CDS, at 837,051 to 837,347 _0730 Psyr_0731: Psyr_0731 - CDS, at 837,344 to 838,102 _0731 Position (kb) 835 836 837 838Strain fitness (log2 ratio) -2 -1 0 1at 834.960 kb on - strandat 835.021 kb on + strandat 835.367 kb on + strandat 835.367 kb on + strandat 835.421 kb on + strand, within Psyr_RNA11at 835.421 kb on + strand, within Psyr_RNA11at 835.736 kb on + strandat 835.845 kb on + strandat 835.859 kb on + strandat 835.872 kb on + strandat 835.876 kb on + strandat 835.876 kb on + strandat 835.925 kb on - strandat 835.930 kb on + strandat 835.931 kb on - strandat 835.931 kb on - strandat 835.995 kb on - strandat 835.995 kb on - strandat 836.050 kb on + strandat 836.050 kb on + strandat 836.050 kb on + strandat 836.050 kb on + strandat 836.202 kb on - strand, within Psyr_0729at 836.248 kb on + strand, within Psyr_0729at 836.248 kb on + strand, within Psyr_0729at 836.248 kb on + strand, within Psyr_0729at 836.249 kb on - strand, within Psyr_0729at 836.342 kb on - strand, within Psyr_0729at 836.355 kb on - strand, within Psyr_0729at 836.355 kb on - strand, within Psyr_0729at 836.492 kb on + strand, within Psyr_0729at 836.492 kb on + strand, within Psyr_0729at 836.530 kb on + strand, within Psyr_0729at 836.687 kb on + strand, within Psyr_0729at 836.687 kb on + strand, within Psyr_0729at 836.696 kb on + strand, within Psyr_0729at 836.696 kb on + strand, within Psyr_0729at 836.751 kb on - strand, within Psyr_0729at 836.938 kb on + strand, within Psyr_0729at 836.938 kb on + strand, within Psyr_0729at 836.938 kb on + strand, within Psyr_0729at 837.065 kb on + strandat 837.065 kb on + strandat 837.065 kb on + strandat 837.074 kb on + strandat 837.110 kb on - strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.581 kb on - strand, within Psyr_0731at 837.593 kb on + strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 838.036 kb on + strandat 838.037 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Gossypol-Acetic Acid Complex 10 mM
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834,960 - +0.7
835,021 + +0.3
835,367 + -0.4
835,367 + +0.1
835,421 + Psyr_RNA11 0.64 +0.6
835,421 + Psyr_RNA11 0.64 -2.5
835,736 + -0.6
835,845 + +0.4
835,859 + +0.7
835,872 + -0.8
835,876 + -2.1
835,876 + -1.4
835,925 - -0.6
835,930 + +0.6
835,931 - -0.3
835,931 - -0.6
835,995 - +0.1
835,995 - +0.1
836,050 + -1.8
836,050 + +0.2
836,050 + +1.0
836,050 + -0.3
836,202 - Psyr_0729 0.22 -0.6
836,248 + Psyr_0729 0.26 +0.1
836,248 + Psyr_0729 0.26 -0.6
836,248 + Psyr_0729 0.26 -0.4
836,249 - Psyr_0729 0.26 -0.6
836,342 - Psyr_0729 0.34 -0.3
836,355 - Psyr_0729 0.35 +0.9
836,355 - Psyr_0729 0.35 -0.6
836,492 + Psyr_0729 0.47 +1.2
836,492 + Psyr_0729 0.47 +0.3
836,530 + Psyr_0729 0.50 -1.4
836,687 + Psyr_0729 0.64 -0.2
836,687 + Psyr_0729 0.64 +0.6
836,696 + Psyr_0729 0.64 -2.2
836,696 + Psyr_0729 0.64 +0.1
836,751 - Psyr_0729 0.69 -0.2
836,938 + Psyr_0729 0.85 +1.4
836,938 + Psyr_0729 0.85 +0.6
836,938 + Psyr_0729 0.85 +0.7
837,065 + -0.2
837,065 + +0.6
837,065 + -0.2
837,074 + +1.2
837,110 - Psyr_0730 0.20 +0.1
837,115 + Psyr_0730 0.22 +0.6
837,115 + Psyr_0730 0.22 +0.2
837,115 + Psyr_0730 0.22 -0.6
837,115 + Psyr_0730 0.22 +1.2
837,115 + Psyr_0730 0.22 +0.1
837,115 + Psyr_0730 0.22 +0.3
837,116 - Psyr_0730 0.22 -1.2
837,116 - Psyr_0730 0.22 -1.8
837,116 - Psyr_0730 0.22 +0.3
837,116 - Psyr_0730 0.22 -0.8
837,116 - Psyr_0730 0.22 +0.5
837,116 - Psyr_0730 0.22 -0.2
837,116 - Psyr_0730 0.22 +0.4
837,116 - Psyr_0730 0.22 -0.3
837,116 - Psyr_0730 0.22 +0.1
837,116 - Psyr_0730 0.22 -0.2
837,116 - Psyr_0730 0.22 -0.6
837,116 - Psyr_0730 0.22 -0.8
837,116 - Psyr_0730 0.22 -0.2
837,116 - Psyr_0730 0.22 +0.7
837,116 - Psyr_0730 0.22 -0.2
837,116 - Psyr_0730 0.22 -0.6
837,117 + Psyr_0730 0.22 +0.9
837,117 + Psyr_0730 0.22 +0.4
837,117 + Psyr_0730 0.22 -0.0
837,117 + Psyr_0730 0.22 +0.2
837,118 - Psyr_0730 0.23 -0.7
837,118 - Psyr_0730 0.23 -0.4
837,118 - Psyr_0730 0.23 +0.1
837,118 - Psyr_0730 0.23 +1.2
837,118 - Psyr_0730 0.23 +0.1
837,118 - Psyr_0730 0.23 -0.5
837,118 - Psyr_0730 0.23 +0.4
837,140 - Psyr_0730 0.30 -0.1
837,581 - Psyr_0731 0.31 -1.6
837,593 + Psyr_0731 0.33 +0.5
837,594 - Psyr_0731 0.33 -0.3
837,594 - Psyr_0731 0.33 -0.3
837,594 - Psyr_0731 0.33 -0.8
837,594 - Psyr_0731 0.33 -0.4
838,036 + +0.0
838,037 - -0.4

Or see this region's nucleotide sequence