Experiment: LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgS and insB-3 are separated by 15 nucleotides insB-3 and insA-3 overlap by 82 nucleotides insA-3 and yagJ are separated by 57 nucleotides yagJ and yagK are separated by 90 nucleotides yagK and yagL are separated by 271 nucleotides
b4688: ykgS - no description, at 289,653 to 289,857
ykgS
b0274: insB-3 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 289,873 to 290,376
insB-3
b0275: insA-3 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 290,295 to 290,570
insA-3
b0276: yagJ - CP4-6 prophage; predicted protein (RefSeq), at 290,628 to 291,455
yagJ
b0277: yagK - CP4-6 prophage; conserved protein (NCBI), at 291,546 to 292,172
yagK
b0278: yagL - CP4-6 prophage; DNA-binding protein (NCBI), at 292,444 to 293,142
yagL
Position (kb) 290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 289.636 kb on - strand at 289.687 kb on - strand, within ykgS at 290.734 kb on + strand, within yagJ at 290.749 kb on + strand, within yagJ at 290.762 kb on - strand, within yagJ at 290.767 kb on - strand, within yagJ at 290.767 kb on - strand, within yagJ at 290.824 kb on + strand, within yagJ at 290.832 kb on - strand, within yagJ at 290.916 kb on - strand, within yagJ at 290.943 kb on - strand, within yagJ at 290.997 kb on + strand, within yagJ at 291.015 kb on + strand, within yagJ at 291.015 kb on + strand, within yagJ at 291.025 kb on + strand, within yagJ at 291.041 kb on - strand, within yagJ at 291.110 kb on + strand, within yagJ at 291.115 kb on + strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.159 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.169 kb on - strand at 291.169 kb on - strand, within yagJ at 291.178 kb on - strand, within yagJ at 291.249 kb on - strand, within yagJ at 291.249 kb on - strand, within yagJ at 291.268 kb on + strand, within yagJ at 291.344 kb on - strand, within yagJ at 291.374 kb on + strand at 291.435 kb on - strand at 291.435 kb on - strand at 291.446 kb on - strand at 291.446 kb on - strand at 291.449 kb on + strand at 291.514 kb on - strand at 291.545 kb on + strand at 291.545 kb on + strand at 291.630 kb on + strand, within yagK at 291.656 kb on - strand, within yagK at 291.656 kb on - strand, within yagK at 291.664 kb on + strand, within yagK at 291.664 kb on + strand, within yagK at 291.667 kb on + strand, within yagK at 291.673 kb on + strand, within yagK at 291.681 kb on - strand, within yagK at 291.708 kb on + strand, within yagK at 291.713 kb on + strand, within yagK at 291.713 kb on + strand, within yagK at 291.721 kb on - strand, within yagK at 291.721 kb on - strand, within yagK at 291.725 kb on + strand, within yagK at 291.735 kb on - strand, within yagK at 291.737 kb on + strand, within yagK at 291.737 kb on + strand, within yagK at 291.737 kb on + strand, within yagK at 291.737 kb on + strand, within yagK at 291.737 kb on + strand, within yagK at 291.747 kb on - strand, within yagK at 291.747 kb on - strand, within yagK at 291.750 kb on + strand, within yagK at 291.758 kb on - strand, within yagK at 291.758 kb on - strand, within yagK at 291.758 kb on - strand, within yagK at 291.766 kb on - strand, within yagK at 291.768 kb on - strand, within yagK at 291.768 kb on - strand, within yagK at 291.773 kb on + strand, within yagK at 291.773 kb on + strand, within yagK at 291.781 kb on - strand, within yagK at 291.781 kb on - strand, within yagK at 291.800 kb on - strand, within yagK at 291.807 kb on + strand, within yagK at 291.807 kb on + strand, within yagK at 291.809 kb on + strand, within yagK at 291.819 kb on - strand, within yagK at 291.856 kb on - strand, within yagK at 291.868 kb on + strand, within yagK at 291.868 kb on + strand, within yagK at 291.891 kb on - strand, within yagK at 291.906 kb on + strand, within yagK at 291.919 kb on + strand, within yagK at 291.921 kb on - strand, within yagK at 291.927 kb on + strand, within yagK at 291.929 kb on + strand, within yagK at 291.929 kb on + strand, within yagK at 291.929 kb on + strand, within yagK at 291.932 kb on + strand, within yagK at 291.935 kb on + strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.937 kb on - strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.946 kb on + strand, within yagK at 291.948 kb on + strand, within yagK at 291.948 kb on + strand, within yagK at 291.951 kb on + strand, within yagK at 291.951 kb on + strand, within yagK at 291.953 kb on + strand, within yagK at 291.954 kb on - strand, within yagK at 291.956 kb on - strand, within yagK at 291.956 kb on - strand, within yagK at 291.958 kb on + strand, within yagK at 291.958 kb on + strand, within yagK at 291.961 kb on - strand, within yagK at 291.961 kb on - strand, within yagK at 291.961 kb on - strand, within yagK at 291.969 kb on - strand, within yagK at 291.977 kb on + strand, within yagK at 291.977 kb on + strand, within yagK at 291.977 kb on + strand, within yagK at 291.981 kb on + strand, within yagK at 291.984 kb on + strand, within yagK at 291.984 kb on + strand, within yagK at 291.995 kb on + strand, within yagK at 292.050 kb on + strand, within yagK at 292.055 kb on + strand, within yagK at 292.055 kb on + strand, within yagK at 292.064 kb on + strand, within yagK at 292.067 kb on + strand, within yagK at 292.067 kb on + strand, within yagK at 292.067 kb on + strand, within yagK at 292.076 kb on + strand, within yagK at 292.140 kb on + strand at 292.140 kb on + strand at 292.145 kb on - strand at 292.150 kb on - strand at 292.160 kb on + strand at 292.160 kb on + strand at 292.174 kb on + strand at 292.182 kb on - strand at 292.191 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on + strand at 292.199 kb on - strand at 292.201 kb on + strand at 292.201 kb on + strand at 292.201 kb on - strand at 292.201 kb on - strand at 292.203 kb on + strand at 292.206 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.207 kb on - strand at 292.209 kb on - strand at 292.209 kb on - strand at 292.209 kb on - strand at 292.210 kb on + strand at 292.210 kb on + strand at 292.211 kb on + strand at 292.211 kb on + strand at 292.211 kb on + strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.211 kb on - strand at 292.213 kb on + strand at 292.213 kb on + strand at 292.213 kb on + strand at 292.218 kb on + strand at 292.218 kb on - strand at 292.218 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.219 kb on - strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on + strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.221 kb on - strand at 292.223 kb on + strand at 292.224 kb on + strand at 292.225 kb on + strand at 292.225 kb on + strand at 292.226 kb on + strand at 292.226 kb on - strand at 292.226 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.229 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.231 kb on - strand at 292.232 kb on - strand at 292.232 kb on - strand at 292.233 kb on - strand at 292.235 kb on + strand at 292.235 kb on + strand at 292.238 kb on - strand at 292.244 kb on - strand at 292.260 kb on + strand at 292.260 kb on + strand at 292.260 kb on + strand at 292.288 kb on + strand at 292.291 kb on + strand at 292.291 kb on + strand at 292.296 kb on + strand at 292.301 kb on + strand at 292.301 kb on + strand at 292.312 kb on + strand at 292.317 kb on + strand at 292.317 kb on + strand at 292.334 kb on + strand at 292.388 kb on + strand at 292.395 kb on - strand at 292.395 kb on - strand at 292.400 kb on - strand at 292.400 kb on - strand at 292.410 kb on - strand at 292.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM remove 289,636 - +0.5 289,687 - ykgS b4688 0.17 -1.6 290,734 + yagJ b0276 0.13 +0.6 290,749 + yagJ b0276 0.15 +1.2 290,762 - yagJ b0276 0.16 -0.8 290,767 - yagJ b0276 0.17 -0.4 290,767 - yagJ b0276 0.17 +0.7 290,824 + yagJ b0276 0.24 +0.7 290,832 - yagJ b0276 0.25 +0.4 290,916 - yagJ b0276 0.35 -0.8 290,943 - yagJ b0276 0.38 -0.6 290,997 + yagJ b0276 0.45 +0.5 291,015 + yagJ b0276 0.47 +1.2 291,015 + yagJ b0276 0.47 -0.0 291,025 + yagJ b0276 0.48 +0.6 291,041 - yagJ b0276 0.50 -0.0 291,110 + yagJ b0276 0.58 +0.8 291,115 + yagJ b0276 0.59 +0.5 291,118 - yagJ b0276 0.59 +2.0 291,118 - yagJ b0276 0.59 +0.7 291,118 - yagJ b0276 0.59 -0.8 291,159 + yagJ b0276 0.64 -0.1 291,161 + yagJ b0276 0.64 +1.0 291,161 + yagJ b0276 0.64 +0.2 291,161 + yagJ b0276 0.64 +0.6 291,161 + yagJ b0276 0.64 +1.4 291,161 + yagJ b0276 0.64 +0.8 291,169 - +1.0 291,169 - yagJ b0276 0.65 -0.8 291,178 - yagJ b0276 0.66 -0.2 291,249 - yagJ b0276 0.75 -1.3 291,249 - yagJ b0276 0.75 +1.0 291,268 + yagJ b0276 0.77 +0.6 291,344 - yagJ b0276 0.86 +0.5 291,374 + +0.4 291,435 - -0.6 291,435 - -0.2 291,446 - -0.3 291,446 - -0.1 291,449 + -0.3 291,514 - -0.9 291,545 + +1.0 291,545 + +0.2 291,630 + yagK b0277 0.13 +0.2 291,656 - yagK b0277 0.18 -1.0 291,656 - yagK b0277 0.18 -1.0 291,664 + yagK b0277 0.19 -0.3 291,664 + yagK b0277 0.19 +2.8 291,667 + yagK b0277 0.19 -1.0 291,673 + yagK b0277 0.20 -0.0 291,681 - yagK b0277 0.22 +0.2 291,708 + yagK b0277 0.26 +0.8 291,713 + yagK b0277 0.27 +0.8 291,713 + yagK b0277 0.27 -0.7 291,721 - yagK b0277 0.28 +1.0 291,721 - yagK b0277 0.28 -0.0 291,725 + yagK b0277 0.29 +0.2 291,735 - yagK b0277 0.30 -0.4 291,737 + yagK b0277 0.30 -2.1 291,737 + yagK b0277 0.30 -1.1 291,737 + yagK b0277 0.30 +1.0 291,737 + yagK b0277 0.30 +1.0 291,737 + yagK b0277 0.30 -0.0 291,747 - yagK b0277 0.32 +0.1 291,747 - yagK b0277 0.32 +0.7 291,750 + yagK b0277 0.33 +0.7 291,758 - yagK b0277 0.34 +0.7 291,758 - yagK b0277 0.34 +0.5 291,758 - yagK b0277 0.34 +0.9 291,766 - yagK b0277 0.35 +0.2 291,768 - yagK b0277 0.35 +0.1 291,768 - yagK b0277 0.35 +0.1 291,773 + yagK b0277 0.36 -0.4 291,773 + yagK b0277 0.36 -1.2 291,781 - yagK b0277 0.37 -0.6 291,781 - yagK b0277 0.37 +0.6 291,800 - yagK b0277 0.41 -0.9 291,807 + yagK b0277 0.42 +0.6 291,807 + yagK b0277 0.42 +0.3 291,809 + yagK b0277 0.42 +1.0 291,819 - yagK b0277 0.44 -3.3 291,856 - yagK b0277 0.49 -1.2 291,868 + yagK b0277 0.51 +0.5 291,868 + yagK b0277 0.51 +0.5 291,891 - yagK b0277 0.55 -0.4 291,906 + yagK b0277 0.57 -0.4 291,919 + yagK b0277 0.59 -0.7 291,921 - yagK b0277 0.60 +0.6 291,927 + yagK b0277 0.61 -0.6 291,929 + yagK b0277 0.61 -0.9 291,929 + yagK b0277 0.61 -1.9 291,929 + yagK b0277 0.61 -0.0 291,932 + yagK b0277 0.62 -1.6 291,935 + yagK b0277 0.62 +0.6 291,937 - yagK b0277 0.62 +0.2 291,937 - yagK b0277 0.62 -0.3 291,937 - yagK b0277 0.62 +1.1 291,937 - yagK b0277 0.62 +0.7 291,937 - yagK b0277 0.62 -0.6 291,937 - yagK b0277 0.62 +0.5 291,937 - yagK b0277 0.62 -0.4 291,946 + yagK b0277 0.64 -0.0 291,946 + yagK b0277 0.64 -0.0 291,946 + yagK b0277 0.64 -0.5 291,946 + yagK b0277 0.64 +1.0 291,946 + yagK b0277 0.64 -1.9 291,946 + yagK b0277 0.64 +0.8 291,946 + yagK b0277 0.64 +1.3 291,946 + yagK b0277 0.64 -1.6 291,948 + yagK b0277 0.64 -1.3 291,948 + yagK b0277 0.64 +2.3 291,951 + yagK b0277 0.65 -0.2 291,951 + yagK b0277 0.65 -1.0 291,953 + yagK b0277 0.65 +0.1 291,954 - yagK b0277 0.65 +1.0 291,956 - yagK b0277 0.65 +0.7 291,956 - yagK b0277 0.65 -0.6 291,958 + yagK b0277 0.66 +0.7 291,958 + yagK b0277 0.66 +0.6 291,961 - yagK b0277 0.66 -1.5 291,961 - yagK b0277 0.66 -0.0 291,961 - yagK b0277 0.66 +2.0 291,969 - yagK b0277 0.67 -0.3 291,977 + yagK b0277 0.69 +0.1 291,977 + yagK b0277 0.69 -0.2 291,977 + yagK b0277 0.69 +0.7 291,981 + yagK b0277 0.69 +1.0 291,984 + yagK b0277 0.70 -0.2 291,984 + yagK b0277 0.70 +0.6 291,995 + yagK b0277 0.72 +2.6 292,050 + yagK b0277 0.80 -0.6 292,055 + yagK b0277 0.81 +0.2 292,055 + yagK b0277 0.81 +0.7 292,064 + yagK b0277 0.83 -1.9 292,067 + yagK b0277 0.83 +1.0 292,067 + yagK b0277 0.83 -1.3 292,067 + yagK b0277 0.83 +1.3 292,076 + yagK b0277 0.85 +0.6 292,140 + +0.4 292,140 + +1.7 292,145 - +1.0 292,150 - +1.0 292,160 + +1.0 292,160 + +1.7 292,174 + -0.0 292,182 - +1.0 292,191 + -1.3 292,199 + +1.0 292,199 + +1.0 292,199 + -0.0 292,199 + +1.0 292,199 + +1.0 292,199 - -0.2 292,201 + +1.0 292,201 + -0.3 292,201 - -0.5 292,201 - -0.0 292,203 + -1.0 292,206 - +1.0 292,207 - +0.9 292,207 - +0.8 292,207 - -0.6 292,207 - -1.8 292,207 - -0.0 292,207 - +0.4 292,207 - -1.1 292,207 - -0.2 292,207 - -0.0 292,207 - +0.3 292,207 - +1.6 292,207 - +0.6 292,207 - +1.1 292,207 - -1.0 292,207 - +0.6 292,207 - -0.0 292,207 - -0.6 292,207 - -0.5 292,207 - -0.0 292,207 - -0.0 292,207 - +0.3 292,207 - +0.7 292,207 - +0.2 292,207 - -0.6 292,207 - +0.4 292,207 - +0.0 292,207 - +0.5 292,209 - -1.1 292,209 - +1.0 292,209 - -1.0 292,210 + -0.0 292,210 + -0.0 292,211 + +1.0 292,211 + +1.0 292,211 + -0.6 292,211 - +0.2 292,211 - +2.4 292,211 - +0.8 292,211 - +0.1 292,211 - +0.7 292,211 - +0.1 292,211 - -0.7 292,211 - -0.2 292,211 - -0.2 292,211 - -0.6 292,211 - +0.2 292,211 - -1.3 292,213 + +1.0 292,213 + +1.0 292,213 + +1.0 292,218 + -0.0 292,218 - -2.0 292,218 - -0.3 292,219 - -2.3 292,219 - -0.0 292,219 - -0.3 292,219 - +1.0 292,219 - -1.2 292,221 + +0.3 292,221 + +1.6 292,221 + +2.0 292,221 + -0.6 292,221 + +1.2 292,221 + -1.3 292,221 + +0.4 292,221 - +2.6 292,221 - +0.8 292,221 - -0.6 292,221 - -0.0 292,223 + -0.0 292,224 + +1.0 292,225 + +1.0 292,225 + +1.0 292,226 + +1.0 292,226 - -1.6 292,226 - +2.0 292,229 - -0.6 292,229 - +1.7 292,229 - +0.4 292,229 - -1.6 292,231 - +1.0 292,231 - +1.7 292,231 - -0.6 292,231 - +0.8 292,232 - -0.0 292,232 - +2.0 292,233 - -0.4 292,235 + +1.0 292,235 + +1.0 292,238 - +1.0 292,244 - +1.0 292,260 + +1.0 292,260 + +1.0 292,260 + +1.0 292,288 + +1.0 292,291 + +1.0 292,291 + -0.0 292,296 + +1.0 292,301 + +1.0 292,301 + -0.6 292,312 + +1.0 292,317 + +1.0 292,317 + +1.0 292,334 + +1.0 292,388 + +1.0 292,395 - +1.0 292,395 - +1.0 292,400 - +1.0 292,400 - +1.0 292,410 - +1.0 292,410 - +1.0
Or see this region's nucleotide sequence