Strain Fitness in Escherichia coli BW25113 around b0978

Experiment: LB Anaerobic with Levofloxacin 0.00000003125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 nthyaE and hyaF overlap by 4 nucleotideshyaF and appC are separated by 133 nucleotidesappC and appB are separated by 11 nucleotides b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975 hyaE b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829 hyaF b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507 appC b0979: appB - cytochrome bd-II oxidase, subunit II (NCBI), at 1,038,519 to 1,039,655 appB Position (kb) 1036 1037 1038 1039Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1035.990 kb on + strandat 1036.062 kb on - strand, within hyaFat 1036.216 kb on + strand, within hyaFat 1036.216 kb on + strand, within hyaFat 1036.275 kb on + strand, within hyaFat 1036.294 kb on + strand, within hyaFat 1036.394 kb on - strand, within hyaFat 1036.463 kb on + strandat 1036.690 kb on - strand, within hyaFat 1036.690 kb on - strand, within hyaFat 1036.774 kb on - strandat 1036.791 kb on - strandat 1036.863 kb on - strandat 1036.863 kb on - strandat 1037.041 kb on - strandat 1037.175 kb on + strand, within appCat 1037.240 kb on - strand, within appCat 1037.240 kb on - strand, within appCat 1037.262 kb on + strand, within appCat 1037.266 kb on + strand, within appCat 1037.367 kb on - strand, within appCat 1037.378 kb on + strand, within appCat 1037.396 kb on - strand, within appCat 1037.766 kb on + strand, within appCat 1037.904 kb on - strand, within appCat 1037.904 kb on - strand, within appCat 1037.934 kb on - strand, within appCat 1037.988 kb on - strand, within appCat 1037.988 kb on - strand, within appCat 1038.009 kb on - strand, within appCat 1038.093 kb on - strand, within appCat 1038.110 kb on - strand, within appCat 1038.170 kb on - strand, within appCat 1038.224 kb on - strand, within appCat 1038.248 kb on - strand, within appCat 1038.293 kb on - strand, within appCat 1038.293 kb on - strand, within appCat 1038.327 kb on - strand, within appCat 1038.336 kb on + strand, within appCat 1038.377 kb on + strandat 1038.395 kb on - strandat 1038.805 kb on - strand, within appBat 1039.201 kb on + strand, within appBat 1039.201 kb on + strand, within appBat 1039.201 kb on + strand, within appBat 1039.209 kb on - strand, within appBat 1039.209 kb on - strand, within appBat 1039.263 kb on + strand, within appBat 1039.453 kb on - strand, within appBat 1039.453 kb on - strand, within appB

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Levofloxacin 0.00000003125 mM
remove
1,035,990 + -0.2
1,036,062 - hyaF b0977 0.10 -1.2
1,036,216 + hyaF b0977 0.28 -0.8
1,036,216 + hyaF b0977 0.28 -0.0
1,036,275 + hyaF b0977 0.35 -0.0
1,036,294 + hyaF b0977 0.38 +0.3
1,036,394 - hyaF b0977 0.49 +0.6
1,036,463 + +0.6
1,036,690 - hyaF b0977 0.84 -1.1
1,036,690 - hyaF b0977 0.84 -1.1
1,036,774 - -0.3
1,036,791 - -0.1
1,036,863 - +0.6
1,036,863 - +0.2
1,037,041 - +0.1
1,037,175 + appC b0978 0.14 +0.8
1,037,240 - appC b0978 0.18 -0.0
1,037,240 - appC b0978 0.18 -0.8
1,037,262 + appC b0978 0.19 +0.4
1,037,266 + appC b0978 0.20 -1.4
1,037,367 - appC b0978 0.26 +0.2
1,037,378 + appC b0978 0.27 +1.0
1,037,396 - appC b0978 0.28 -0.1
1,037,766 + appC b0978 0.52 -1.0
1,037,904 - appC b0978 0.61 +1.3
1,037,904 - appC b0978 0.61 +2.7
1,037,934 - appC b0978 0.63 +0.9
1,037,988 - appC b0978 0.66 +0.8
1,037,988 - appC b0978 0.66 +0.7
1,038,009 - appC b0978 0.68 -0.7
1,038,093 - appC b0978 0.73 +0.1
1,038,110 - appC b0978 0.74 +0.3
1,038,170 - appC b0978 0.78 +0.4
1,038,224 - appC b0978 0.82 -1.0
1,038,248 - appC b0978 0.83 +1.1
1,038,293 - appC b0978 0.86 +0.8
1,038,293 - appC b0978 0.86 -0.0
1,038,327 - appC b0978 0.88 +0.4
1,038,336 + appC b0978 0.89 -0.5
1,038,377 + -1.9
1,038,395 - -0.1
1,038,805 - appB b0979 0.25 +0.0
1,039,201 + appB b0979 0.60 +0.5
1,039,201 + appB b0979 0.60 -0.1
1,039,201 + appB b0979 0.60 +0.4
1,039,209 - appB b0979 0.61 +1.3
1,039,209 - appB b0979 0.61 +1.1
1,039,263 + appB b0979 0.65 +2.9
1,039,453 - appB b0979 0.82 -0.3
1,039,453 - appB b0979 0.82 +1.0

Or see this region's nucleotide sequence