Experiment: LB Anaerobic with Levofloxacin 0.0000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfP and ymfQ overlap by 10 nucleotides ymfQ and ycfK are separated by 3 nucleotides ycfK and ymfS are separated by 1 nucleotides ymfS and tfaE overlap by 29 nucleotides tfaE and stfE overlap by 1 nucleotides
b1152: ymfP - orf, hypothetical protein (VIMSS), at 1,205,366 to 1,206,145
ymfP
b1153: ymfQ - e14 prophage; conserved protein (NCBI), at 1,206,136 to 1,206,720
ymfQ
b1154: ycfK - e14 prophage; predicted protein (NCBI), at 1,206,724 to 1,207,353
ycfK
b1155: ymfS - e14 prophage; predicted protein (NCBI), at 1,207,355 to 1,207,768
ymfS
b1156: tfaE - e14 prophage; predicted tail fiber assembly protein (NCBI), at 1,207,740 to 1,208,342
tfaE
b1157: stfE - no description, at 1,208,342 to 1,208,842
stfE
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1205.740 kb on - strand, within ymfP at 1205.869 kb on - strand, within ymfP at 1206.162 kb on - strand at 1206.273 kb on + strand, within ymfQ at 1206.457 kb on + strand, within ymfQ at 1206.660 kb on - strand, within ymfQ at 1206.672 kb on - strand at 1207.069 kb on + strand, within ycfK at 1207.070 kb on + strand, within ycfK at 1207.076 kb on + strand, within ycfK at 1207.084 kb on - strand, within ycfK at 1207.097 kb on + strand, within ycfK at 1207.147 kb on + strand, within ycfK at 1207.155 kb on + strand, within ycfK at 1207.156 kb on + strand, within ycfK at 1207.164 kb on + strand, within ycfK at 1207.169 kb on + strand, within ycfK at 1207.177 kb on + strand, within ycfK at 1207.177 kb on + strand, within ycfK at 1207.192 kb on + strand, within ycfK at 1207.246 kb on + strand, within ycfK at 1207.248 kb on + strand, within ycfK at 1207.248 kb on + strand, within ycfK at 1207.258 kb on + strand, within ycfK at 1207.258 kb on + strand, within ycfK at 1207.260 kb on + strand, within ycfK at 1207.267 kb on + strand, within ycfK at 1207.270 kb on + strand, within ycfK at 1207.291 kb on + strand at 1207.291 kb on + strand at 1207.339 kb on + strand at 1207.390 kb on + strand at 1207.390 kb on + strand at 1207.540 kb on - strand, within ymfS at 1207.545 kb on - strand, within ymfS at 1207.563 kb on + strand, within ymfS at 1207.605 kb on + strand, within ymfS at 1207.617 kb on + strand, within ymfS at 1207.617 kb on + strand, within ymfS at 1207.624 kb on - strand, within ymfS at 1207.624 kb on - strand, within ymfS at 1207.626 kb on + strand, within ymfS at 1207.633 kb on + strand, within ymfS at 1207.649 kb on - strand, within ymfS at 1207.681 kb on + strand, within ymfS at 1207.703 kb on - strand, within ymfS at 1207.746 kb on - strand at 1207.746 kb on - strand at 1207.746 kb on - strand at 1207.746 kb on - strand at 1207.772 kb on - strand at 1207.780 kb on + strand at 1207.780 kb on + strand at 1207.792 kb on + strand at 1207.803 kb on - strand, within tfaE at 1207.815 kb on + strand, within tfaE at 1207.815 kb on + strand, within tfaE at 1207.859 kb on - strand, within tfaE at 1207.959 kb on + strand, within tfaE at 1207.962 kb on - strand, within tfaE at 1207.964 kb on - strand, within tfaE at 1207.967 kb on + strand, within tfaE at 1207.972 kb on + strand, within tfaE at 1208.100 kb on - strand, within tfaE at 1208.167 kb on - strand, within tfaE at 1208.167 kb on - strand, within tfaE at 1208.168 kb on - strand, within tfaE at 1208.168 kb on - strand, within tfaE at 1208.170 kb on + strand, within tfaE at 1208.172 kb on + strand, within tfaE at 1208.177 kb on + strand, within tfaE at 1208.178 kb on - strand, within tfaE at 1208.178 kb on - strand, within tfaE at 1208.180 kb on - strand, within tfaE at 1208.180 kb on - strand, within tfaE at 1208.184 kb on + strand, within tfaE at 1208.185 kb on - strand, within tfaE at 1208.187 kb on - strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Levofloxacin 0.0000000625 mM remove 1,205,740 - ymfP b1152 0.48 +1.3 1,205,869 - ymfP b1152 0.64 +2.1 1,206,162 - +1.3 1,206,273 + ymfQ b1153 0.23 +1.6 1,206,457 + ymfQ b1153 0.55 +1.9 1,206,660 - ymfQ b1153 0.90 +1.0 1,206,672 - -1.2 1,207,069 + ycfK b1154 0.55 +1.8 1,207,070 + ycfK b1154 0.55 +0.7 1,207,076 + ycfK b1154 0.56 +2.1 1,207,084 - ycfK b1154 0.57 +2.8 1,207,097 + ycfK b1154 0.59 -0.5 1,207,147 + ycfK b1154 0.67 +1.0 1,207,155 + ycfK b1154 0.68 +2.7 1,207,156 + ycfK b1154 0.69 -0.9 1,207,164 + ycfK b1154 0.70 +2.9 1,207,169 + ycfK b1154 0.71 +1.3 1,207,177 + ycfK b1154 0.72 -1.4 1,207,177 + ycfK b1154 0.72 +1.5 1,207,192 + ycfK b1154 0.74 +0.6 1,207,246 + ycfK b1154 0.83 +2.5 1,207,248 + ycfK b1154 0.83 +0.7 1,207,248 + ycfK b1154 0.83 -0.0 1,207,258 + ycfK b1154 0.85 +1.2 1,207,258 + ycfK b1154 0.85 +1.6 1,207,260 + ycfK b1154 0.85 +1.4 1,207,267 + ycfK b1154 0.86 +1.8 1,207,270 + ycfK b1154 0.87 +0.5 1,207,291 + +0.6 1,207,291 + +0.9 1,207,339 + +1.7 1,207,390 + +0.2 1,207,390 + -0.2 1,207,540 - ymfS b1155 0.45 -0.2 1,207,545 - ymfS b1155 0.46 +3.8 1,207,563 + ymfS b1155 0.50 +0.8 1,207,605 + ymfS b1155 0.60 -1.8 1,207,617 + ymfS b1155 0.63 +1.0 1,207,617 + ymfS b1155 0.63 -0.0 1,207,624 - ymfS b1155 0.65 +2.5 1,207,624 - ymfS b1155 0.65 +0.8 1,207,626 + ymfS b1155 0.65 +1.1 1,207,633 + ymfS b1155 0.67 -2.0 1,207,649 - ymfS b1155 0.71 +0.8 1,207,681 + ymfS b1155 0.79 -0.2 1,207,703 - ymfS b1155 0.84 +0.8 1,207,746 - +1.5 1,207,746 - +1.0 1,207,746 - +2.3 1,207,746 - +0.3 1,207,772 - +2.2 1,207,780 + -0.2 1,207,780 + -0.8 1,207,792 + +2.1 1,207,803 - tfaE b1156 0.10 -0.2 1,207,815 + tfaE b1156 0.12 +1.8 1,207,815 + tfaE b1156 0.12 -0.2 1,207,859 - tfaE b1156 0.20 +2.2 1,207,959 + tfaE b1156 0.36 -0.2 1,207,962 - tfaE b1156 0.37 +2.1 1,207,964 - tfaE b1156 0.37 -0.2 1,207,967 + tfaE b1156 0.38 -0.8 1,207,972 + tfaE b1156 0.38 +0.8 1,208,100 - tfaE b1156 0.60 +1.8 1,208,167 - tfaE b1156 0.71 -0.7 1,208,167 - tfaE b1156 0.71 +0.8 1,208,168 - tfaE b1156 0.71 +0.8 1,208,168 - tfaE b1156 0.71 +2.0 1,208,170 + tfaE b1156 0.71 +0.8 1,208,172 + tfaE b1156 0.72 +0.8 1,208,177 + tfaE b1156 0.72 -0.2 1,208,178 - tfaE b1156 0.73 +2.4 1,208,178 - tfaE b1156 0.73 +1.4 1,208,180 - tfaE b1156 0.73 -0.8 1,208,180 - tfaE b1156 0.73 -0.2 1,208,184 + tfaE b1156 0.74 +0.8 1,208,185 - tfaE b1156 0.74 -0.8 1,208,187 - tfaE b1156 0.74 +1.4 1,208,194 + tfaE b1156 0.75 +0.8 1,208,194 + tfaE b1156 0.75 +0.8 1,208,194 + tfaE b1156 0.75 +0.8 1,208,352 - +0.8
Or see this region's nucleotide sequence