Experiment: LB Anaerobic with Levofloxacin 0.0000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aaaE and ymfR are separated by 11 nucleotides ymfR and ymfO overlap by 1 nucleotides ymfO and ymfP are separated by 0 nucleotides ymfP and ymfQ overlap by 10 nucleotides ymfQ and ycfK are separated by 3 nucleotides
b4693: aaaE - no description, at 1,203,627 to 1,204,760
aaaE
b1150: ymfR - e14 prophage; predicted protein (NCBI), at 1,204,772 to 1,204,954
ymfR
b1151: ymfO - e14 prophage; conserved protein (RefSeq), at 1,204,954 to 1,205,365
ymfO
b1152: ymfP - orf, hypothetical protein (VIMSS), at 1,205,366 to 1,206,145
ymfP
b1153: ymfQ - e14 prophage; conserved protein (NCBI), at 1,206,136 to 1,206,720
ymfQ
b1154: ycfK - e14 prophage; predicted protein (NCBI), at 1,206,724 to 1,207,353
ycfK
Position (kb)
1205
1206
1207 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1204.391 kb on - strand, within aaaE at 1204.420 kb on - strand, within aaaE at 1204.462 kb on + strand, within aaaE at 1204.841 kb on - strand, within ymfR at 1204.996 kb on + strand, within ymfO at 1205.042 kb on - strand, within ymfO at 1205.128 kb on + strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.409 kb on + strand at 1205.409 kb on + strand at 1205.435 kb on - strand at 1205.437 kb on - strand at 1205.474 kb on + strand, within ymfP at 1205.474 kb on + strand, within ymfP at 1205.630 kb on + strand, within ymfP at 1205.661 kb on - strand, within ymfP at 1205.709 kb on - strand, within ymfP at 1205.740 kb on - strand, within ymfP at 1205.869 kb on - strand, within ymfP at 1206.162 kb on - strand at 1206.273 kb on + strand, within ymfQ at 1206.457 kb on + strand, within ymfQ at 1206.660 kb on - strand, within ymfQ at 1206.672 kb on - strand at 1207.069 kb on + strand, within ycfK at 1207.070 kb on + strand, within ycfK at 1207.076 kb on + strand, within ycfK at 1207.084 kb on - strand, within ycfK at 1207.097 kb on + strand, within ycfK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Levofloxacin 0.0000000625 mM remove 1,204,391 - aaaE b4693 0.67 +0.9 1,204,420 - aaaE b4693 0.70 +0.5 1,204,462 + aaaE b4693 0.74 +2.1 1,204,841 - ymfR b1150 0.38 +1.7 1,204,996 + ymfO b1151 0.10 +0.8 1,205,042 - ymfO b1151 0.21 +1.0 1,205,128 + ymfO b1151 0.42 +0.9 1,205,226 - ymfO b1151 0.66 +1.7 1,205,226 - ymfO b1151 0.66 +0.8 1,205,409 + +0.3 1,205,409 + +0.3 1,205,435 - +1.9 1,205,437 - +0.4 1,205,474 + ymfP b1152 0.14 +3.4 1,205,474 + ymfP b1152 0.14 +0.9 1,205,630 + ymfP b1152 0.34 +1.0 1,205,661 - ymfP b1152 0.38 +1.9 1,205,709 - ymfP b1152 0.44 +0.5 1,205,740 - ymfP b1152 0.48 +1.3 1,205,869 - ymfP b1152 0.64 +2.1 1,206,162 - +1.3 1,206,273 + ymfQ b1153 0.23 +1.6 1,206,457 + ymfQ b1153 0.55 +1.9 1,206,660 - ymfQ b1153 0.90 +1.0 1,206,672 - -1.2 1,207,069 + ycfK b1154 0.55 +1.8 1,207,070 + ycfK b1154 0.55 +0.7 1,207,076 + ycfK b1154 0.56 +2.1 1,207,084 - ycfK b1154 0.57 +2.8 1,207,097 + ycfK b1154 0.59 -0.5
Or see this region's nucleotide sequence