Strain Fitness in Escherichia coli BW25113 around b3478
Experiment: LB Anaerobic with Irgasan 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Anaerobic with Irgasan 0.000000625 mM |
---|---|---|---|---|---|
remove | |||||
3,613,211 | - | -1.4 | |||
3,613,211 | - | +0.0 | |||
3,613,251 | - | -0.1 | |||
3,613,251 | - | +0.0 | |||
3,613,252 | + | -1.6 | |||
3,613,309 | + | -1.0 | |||
3,613,309 | + | -1.7 | |||
3,613,360 | - | nikB | b3477 | 0.10 | -1.6 |
3,613,403 | + | nikB | b3477 | 0.15 | +1.3 |
3,613,403 | + | nikB | b3477 | 0.15 | -1.9 |
3,613,445 | + | nikB | b3477 | 0.19 | +0.2 |
3,613,450 | + | nikB | b3477 | 0.20 | -0.3 |
3,613,461 | - | nikB | b3477 | 0.21 | +0.5 |
3,613,461 | - | nikB | b3477 | 0.21 | -0.6 |
3,613,467 | - | nikB | b3477 | 0.21 | +0.7 |
3,613,531 | + | nikB | b3477 | 0.28 | -1.2 |
3,613,539 | - | nikB | b3477 | 0.29 | -1.2 |
3,613,539 | - | nikB | b3477 | 0.29 | -0.8 |
3,613,539 | - | nikB | b3477 | 0.29 | -1.4 |
3,613,539 | - | nikB | b3477 | 0.29 | +0.7 |
3,613,539 | - | nikB | b3477 | 0.29 | -1.0 |
3,613,539 | - | nikB | b3477 | 0.29 | -3.3 |
3,613,539 | - | nikB | b3477 | 0.29 | +0.3 |
3,613,544 | + | nikB | b3477 | 0.30 | +0.6 |
3,613,551 | - | nikB | b3477 | 0.30 | -0.3 |
3,613,592 | - | nikB | b3477 | 0.35 | +0.2 |
3,613,607 | + | nikB | b3477 | 0.36 | +0.2 |
3,613,607 | + | nikB | b3477 | 0.36 | -1.1 |
3,613,607 | + | nikB | b3477 | 0.36 | -1.3 |
3,613,615 | - | nikB | b3477 | 0.37 | -1.2 |
3,613,615 | - | nikB | b3477 | 0.37 | -0.5 |
3,613,670 | + | nikB | b3477 | 0.43 | -0.4 |
3,613,670 | + | nikB | b3477 | 0.43 | -0.8 |
3,613,691 | + | nikB | b3477 | 0.45 | -0.6 |
3,613,773 | - | nikB | b3477 | 0.54 | -1.1 |
3,613,796 | - | nikB | b3477 | 0.56 | -0.1 |
3,613,848 | - | nikB | b3477 | 0.62 | -0.8 |
3,613,848 | - | nikB | b3477 | 0.62 | -0.2 |
3,613,869 | + | nikB | b3477 | 0.64 | -0.4 |
3,613,874 | - | nikB | b3477 | 0.65 | -0.1 |
3,613,878 | + | nikB | b3477 | 0.65 | -0.3 |
3,613,881 | - | nikB | b3477 | 0.65 | -0.7 |
3,613,932 | + | nikB | b3477 | 0.71 | -0.2 |
3,613,934 | + | nikB | b3477 | 0.71 | -1.1 |
3,613,942 | + | nikB | b3477 | 0.72 | -0.6 |
3,613,942 | - | nikB | b3477 | 0.72 | -0.7 |
3,613,947 | - | nikB | b3477 | 0.72 | -1.3 |
3,613,947 | - | nikB | b3477 | 0.72 | -0.2 |
3,613,988 | + | nikB | b3477 | 0.77 | -0.1 |
3,614,094 | + | nikB | b3477 | 0.88 | -0.8 |
3,614,249 | + | +0.0 | |||
3,614,249 | + | -1.3 | |||
3,614,272 | + | +0.0 | |||
3,614,273 | + | -2.1 | |||
3,614,286 | + | -0.2 | |||
3,614,290 | + | nikC | b3478 | 0.10 | -0.9 |
3,614,316 | - | nikC | b3478 | 0.13 | -0.9 |
3,614,352 | + | nikC | b3478 | 0.18 | -2.2 |
3,614,371 | - | nikC | b3478 | 0.20 | -0.7 |
3,614,376 | + | nikC | b3478 | 0.21 | -0.7 |
3,614,432 | - | nikC | b3478 | 0.27 | -0.3 |
3,614,432 | - | nikC | b3478 | 0.27 | +0.2 |
3,614,436 | + | nikC | b3478 | 0.28 | -0.4 |
3,614,443 | + | nikC | b3478 | 0.29 | -1.2 |
3,614,443 | + | nikC | b3478 | 0.29 | -0.1 |
3,614,444 | - | nikC | b3478 | 0.29 | +0.3 |
3,614,446 | + | nikC | b3478 | 0.29 | -1.4 |
3,614,543 | + | nikC | b3478 | 0.41 | -0.1 |
3,614,551 | - | nikC | b3478 | 0.41 | -0.9 |
3,614,559 | + | nikC | b3478 | 0.42 | +0.8 |
3,614,639 | - | nikC | b3478 | 0.52 | +0.6 |
3,614,661 | + | nikC | b3478 | 0.55 | +0.6 |
3,614,661 | + | nikC | b3478 | 0.55 | +0.9 |
3,614,688 | - | nikC | b3478 | 0.58 | +0.2 |
3,614,688 | - | nikC | b3478 | 0.58 | -0.6 |
3,614,739 | - | nikC | b3478 | 0.64 | -0.4 |
3,614,739 | - | nikC | b3478 | 0.64 | -1.5 |
3,614,751 | + | nikC | b3478 | 0.65 | +0.0 |
3,614,753 | - | nikC | b3478 | 0.66 | -0.5 |
3,614,809 | + | nikC | b3478 | 0.72 | +0.5 |
3,614,868 | + | nikC | b3478 | 0.79 | -2.2 |
3,614,907 | + | nikC | b3478 | 0.84 | -0.1 |
3,614,911 | - | nikC | b3478 | 0.85 | -2.1 |
3,614,938 | - | nikC | b3478 | 0.88 | -1.1 |
3,614,983 | + | -1.1 | |||
3,614,983 | + | -1.0 | |||
3,615,008 | - | +1.0 | |||
3,615,033 | - | -0.2 | |||
3,615,047 | - | -1.2 | |||
3,615,066 | - | -0.3 | |||
3,615,089 | + | -0.3 | |||
3,615,100 | - | -0.7 | |||
3,615,103 | + | -1.2 | |||
3,615,103 | + | -0.5 | |||
3,615,164 | + | nikD | b3479 | 0.16 | -0.8 |
3,615,327 | - | nikD | b3479 | 0.38 | +0.5 |
3,615,330 | - | nikD | b3479 | 0.38 | -0.1 |
3,615,336 | - | nikD | b3479 | 0.39 | -0.3 |
3,615,336 | - | nikD | b3479 | 0.39 | -1.3 |
3,615,336 | - | nikD | b3479 | 0.39 | -0.3 |
3,615,337 | - | nikD | b3479 | 0.39 | -0.6 |
3,615,337 | - | nikD | b3479 | 0.39 | -0.5 |
3,615,337 | - | nikD | b3479 | 0.39 | -0.6 |
3,615,364 | + | nikD | b3479 | 0.43 | -0.4 |
3,615,432 | - | nikD | b3479 | 0.52 | -0.1 |
3,615,443 | - | nikD | b3479 | 0.53 | -1.2 |
3,615,445 | + | nikD | b3479 | 0.53 | -0.3 |
3,615,445 | + | nikD | b3479 | 0.53 | -2.0 |
3,615,500 | + | nikD | b3479 | 0.60 | -1.7 |
3,615,500 | + | nikD | b3479 | 0.60 | -2.2 |
3,615,513 | - | nikD | b3479 | 0.62 | -0.3 |
3,615,528 | + | nikD | b3479 | 0.64 | +0.2 |
3,615,565 | + | nikD | b3479 | 0.69 | -1.6 |
3,615,565 | + | nikD | b3479 | 0.69 | -1.6 |
3,615,573 | + | nikD | b3479 | 0.70 | -0.2 |
3,615,706 | - | nikD | b3479 | 0.87 | -0.7 |
3,615,708 | + | nikD | b3479 | 0.88 | +0.2 |
3,615,708 | + | nikD | b3479 | 0.88 | -0.2 |
3,615,711 | - | nikD | b3479 | 0.88 | -0.1 |
3,615,729 | + | +0.3 | |||
3,615,735 | + | -1.2 | |||
3,615,735 | + | -1.4 | |||
3,615,783 | + | -2.4 | |||
3,615,783 | + | +0.6 | |||
3,615,820 | + | +0.0 | |||
3,615,892 | + | nikE | b3480 | 0.12 | +0.0 |
3,615,892 | + | nikE | b3480 | 0.12 | +0.5 |
3,615,899 | + | nikE | b3480 | 0.12 | +0.4 |
3,615,966 | + | nikE | b3480 | 0.21 | -0.3 |
3,615,966 | + | nikE | b3480 | 0.21 | -1.1 |
3,615,966 | + | nikE | b3480 | 0.21 | -0.5 |
3,615,977 | + | nikE | b3480 | 0.22 | -0.7 |
Or see this region's nucleotide sequence