Experiment: LB Anaerobic with Irgasan 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nikA and nikB overlap by 1 nucleotides nikB and nikC overlap by 4 nucleotides nikC and nikD overlap by 1 nucleotides nikD and nikE overlap by 4 nucleotides
b3476: nikA - nickel transporter subunit (NCBI), at 3,611,690 to 3,613,264
nikA
b3477: nikB - nickel transporter subunit (NCBI), at 3,613,264 to 3,614,208
nikB
b3478: nikC - nickel transporter subunit (NCBI), at 3,614,205 to 3,615,038
nikC
b3479: nikD - nickel transporter subunit (NCBI), at 3,615,038 to 3,615,802
nikD
b3480: nikE - nickel transporter subunit (NCBI), at 3,615,799 to 3,616,605
nikE
Position (kb)
3614
3615
3616 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3613.211 kb on - strand at 3613.211 kb on - strand at 3613.251 kb on - strand at 3613.251 kb on - strand at 3613.252 kb on + strand at 3613.309 kb on + strand at 3613.309 kb on + strand at 3613.360 kb on - strand, within nikB at 3613.403 kb on + strand, within nikB at 3613.403 kb on + strand, within nikB at 3613.445 kb on + strand, within nikB at 3613.450 kb on + strand, within nikB at 3613.461 kb on - strand, within nikB at 3613.461 kb on - strand, within nikB at 3613.467 kb on - strand, within nikB at 3613.531 kb on + strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.544 kb on + strand, within nikB at 3613.551 kb on - strand, within nikB at 3613.592 kb on - strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.615 kb on - strand, within nikB at 3613.615 kb on - strand, within nikB at 3613.670 kb on + strand, within nikB at 3613.670 kb on + strand, within nikB at 3613.691 kb on + strand, within nikB at 3613.773 kb on - strand, within nikB at 3613.796 kb on - strand, within nikB at 3613.848 kb on - strand, within nikB at 3613.848 kb on - strand, within nikB at 3613.869 kb on + strand, within nikB at 3613.874 kb on - strand, within nikB at 3613.878 kb on + strand, within nikB at 3613.881 kb on - strand, within nikB at 3613.932 kb on + strand, within nikB at 3613.934 kb on + strand, within nikB at 3613.942 kb on + strand, within nikB at 3613.942 kb on - strand, within nikB at 3613.947 kb on - strand, within nikB at 3613.947 kb on - strand, within nikB at 3613.988 kb on + strand, within nikB at 3614.094 kb on + strand, within nikB at 3614.249 kb on + strand at 3614.249 kb on + strand at 3614.272 kb on + strand at 3614.273 kb on + strand at 3614.286 kb on + strand at 3614.290 kb on + strand, within nikC at 3614.316 kb on - strand, within nikC at 3614.352 kb on + strand, within nikC at 3614.371 kb on - strand, within nikC at 3614.376 kb on + strand, within nikC at 3614.432 kb on - strand, within nikC at 3614.432 kb on - strand, within nikC at 3614.436 kb on + strand, within nikC at 3614.443 kb on + strand, within nikC at 3614.443 kb on + strand, within nikC at 3614.444 kb on - strand, within nikC at 3614.446 kb on + strand, within nikC at 3614.543 kb on + strand, within nikC at 3614.551 kb on - strand, within nikC at 3614.559 kb on + strand, within nikC at 3614.639 kb on - strand, within nikC at 3614.661 kb on + strand, within nikC at 3614.661 kb on + strand, within nikC at 3614.688 kb on - strand, within nikC at 3614.688 kb on - strand, within nikC at 3614.739 kb on - strand, within nikC at 3614.739 kb on - strand, within nikC at 3614.751 kb on + strand, within nikC at 3614.753 kb on - strand, within nikC at 3614.809 kb on + strand, within nikC at 3614.868 kb on + strand, within nikC at 3614.907 kb on + strand, within nikC at 3614.911 kb on - strand, within nikC at 3614.938 kb on - strand, within nikC at 3614.983 kb on + strand at 3614.983 kb on + strand at 3615.008 kb on - strand at 3615.033 kb on - strand at 3615.047 kb on - strand at 3615.066 kb on - strand at 3615.089 kb on + strand at 3615.100 kb on - strand at 3615.103 kb on + strand at 3615.103 kb on + strand at 3615.164 kb on + strand, within nikD at 3615.327 kb on - strand, within nikD at 3615.330 kb on - strand, within nikD at 3615.336 kb on - strand, within nikD at 3615.336 kb on - strand, within nikD at 3615.336 kb on - strand, within nikD at 3615.337 kb on - strand, within nikD at 3615.337 kb on - strand, within nikD at 3615.337 kb on - strand, within nikD at 3615.364 kb on + strand, within nikD at 3615.432 kb on - strand, within nikD at 3615.443 kb on - strand, within nikD at 3615.445 kb on + strand, within nikD at 3615.445 kb on + strand, within nikD at 3615.500 kb on + strand, within nikD at 3615.500 kb on + strand, within nikD at 3615.513 kb on - strand, within nikD at 3615.528 kb on + strand, within nikD at 3615.565 kb on + strand, within nikD at 3615.565 kb on + strand, within nikD at 3615.573 kb on + strand, within nikD at 3615.706 kb on - strand, within nikD at 3615.708 kb on + strand, within nikD at 3615.708 kb on + strand, within nikD at 3615.711 kb on - strand, within nikD at 3615.729 kb on + strand at 3615.735 kb on + strand at 3615.735 kb on + strand at 3615.783 kb on + strand at 3615.783 kb on + strand at 3615.820 kb on + strand at 3615.892 kb on + strand, within nikE at 3615.892 kb on + strand, within nikE at 3615.899 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.977 kb on + strand, within nikE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Irgasan 0.000000625 mM remove 3,613,211 - -1.4 3,613,211 - +0.0 3,613,251 - -0.1 3,613,251 - +0.0 3,613,252 + -1.6 3,613,309 + -1.0 3,613,309 + -1.7 3,613,360 - nikB b3477 0.10 -1.6 3,613,403 + nikB b3477 0.15 +1.3 3,613,403 + nikB b3477 0.15 -1.9 3,613,445 + nikB b3477 0.19 +0.2 3,613,450 + nikB b3477 0.20 -0.3 3,613,461 - nikB b3477 0.21 +0.5 3,613,461 - nikB b3477 0.21 -0.6 3,613,467 - nikB b3477 0.21 +0.7 3,613,531 + nikB b3477 0.28 -1.2 3,613,539 - nikB b3477 0.29 -1.2 3,613,539 - nikB b3477 0.29 -0.8 3,613,539 - nikB b3477 0.29 -1.4 3,613,539 - nikB b3477 0.29 +0.7 3,613,539 - nikB b3477 0.29 -1.0 3,613,539 - nikB b3477 0.29 -3.3 3,613,539 - nikB b3477 0.29 +0.3 3,613,544 + nikB b3477 0.30 +0.6 3,613,551 - nikB b3477 0.30 -0.3 3,613,592 - nikB b3477 0.35 +0.2 3,613,607 + nikB b3477 0.36 +0.2 3,613,607 + nikB b3477 0.36 -1.1 3,613,607 + nikB b3477 0.36 -1.3 3,613,615 - nikB b3477 0.37 -1.2 3,613,615 - nikB b3477 0.37 -0.5 3,613,670 + nikB b3477 0.43 -0.4 3,613,670 + nikB b3477 0.43 -0.8 3,613,691 + nikB b3477 0.45 -0.6 3,613,773 - nikB b3477 0.54 -1.1 3,613,796 - nikB b3477 0.56 -0.1 3,613,848 - nikB b3477 0.62 -0.8 3,613,848 - nikB b3477 0.62 -0.2 3,613,869 + nikB b3477 0.64 -0.4 3,613,874 - nikB b3477 0.65 -0.1 3,613,878 + nikB b3477 0.65 -0.3 3,613,881 - nikB b3477 0.65 -0.7 3,613,932 + nikB b3477 0.71 -0.2 3,613,934 + nikB b3477 0.71 -1.1 3,613,942 + nikB b3477 0.72 -0.6 3,613,942 - nikB b3477 0.72 -0.7 3,613,947 - nikB b3477 0.72 -1.3 3,613,947 - nikB b3477 0.72 -0.2 3,613,988 + nikB b3477 0.77 -0.1 3,614,094 + nikB b3477 0.88 -0.8 3,614,249 + +0.0 3,614,249 + -1.3 3,614,272 + +0.0 3,614,273 + -2.1 3,614,286 + -0.2 3,614,290 + nikC b3478 0.10 -0.9 3,614,316 - nikC b3478 0.13 -0.9 3,614,352 + nikC b3478 0.18 -2.2 3,614,371 - nikC b3478 0.20 -0.7 3,614,376 + nikC b3478 0.21 -0.7 3,614,432 - nikC b3478 0.27 -0.3 3,614,432 - nikC b3478 0.27 +0.2 3,614,436 + nikC b3478 0.28 -0.4 3,614,443 + nikC b3478 0.29 -1.2 3,614,443 + nikC b3478 0.29 -0.1 3,614,444 - nikC b3478 0.29 +0.3 3,614,446 + nikC b3478 0.29 -1.4 3,614,543 + nikC b3478 0.41 -0.1 3,614,551 - nikC b3478 0.41 -0.9 3,614,559 + nikC b3478 0.42 +0.8 3,614,639 - nikC b3478 0.52 +0.6 3,614,661 + nikC b3478 0.55 +0.6 3,614,661 + nikC b3478 0.55 +0.9 3,614,688 - nikC b3478 0.58 +0.2 3,614,688 - nikC b3478 0.58 -0.6 3,614,739 - nikC b3478 0.64 -0.4 3,614,739 - nikC b3478 0.64 -1.5 3,614,751 + nikC b3478 0.65 +0.0 3,614,753 - nikC b3478 0.66 -0.5 3,614,809 + nikC b3478 0.72 +0.5 3,614,868 + nikC b3478 0.79 -2.2 3,614,907 + nikC b3478 0.84 -0.1 3,614,911 - nikC b3478 0.85 -2.1 3,614,938 - nikC b3478 0.88 -1.1 3,614,983 + -1.1 3,614,983 + -1.0 3,615,008 - +1.0 3,615,033 - -0.2 3,615,047 - -1.2 3,615,066 - -0.3 3,615,089 + -0.3 3,615,100 - -0.7 3,615,103 + -1.2 3,615,103 + -0.5 3,615,164 + nikD b3479 0.16 -0.8 3,615,327 - nikD b3479 0.38 +0.5 3,615,330 - nikD b3479 0.38 -0.1 3,615,336 - nikD b3479 0.39 -0.3 3,615,336 - nikD b3479 0.39 -1.3 3,615,336 - nikD b3479 0.39 -0.3 3,615,337 - nikD b3479 0.39 -0.6 3,615,337 - nikD b3479 0.39 -0.5 3,615,337 - nikD b3479 0.39 -0.6 3,615,364 + nikD b3479 0.43 -0.4 3,615,432 - nikD b3479 0.52 -0.1 3,615,443 - nikD b3479 0.53 -1.2 3,615,445 + nikD b3479 0.53 -0.3 3,615,445 + nikD b3479 0.53 -2.0 3,615,500 + nikD b3479 0.60 -1.7 3,615,500 + nikD b3479 0.60 -2.2 3,615,513 - nikD b3479 0.62 -0.3 3,615,528 + nikD b3479 0.64 +0.2 3,615,565 + nikD b3479 0.69 -1.6 3,615,565 + nikD b3479 0.69 -1.6 3,615,573 + nikD b3479 0.70 -0.2 3,615,706 - nikD b3479 0.87 -0.7 3,615,708 + nikD b3479 0.88 +0.2 3,615,708 + nikD b3479 0.88 -0.2 3,615,711 - nikD b3479 0.88 -0.1 3,615,729 + +0.3 3,615,735 + -1.2 3,615,735 + -1.4 3,615,783 + -2.4 3,615,783 + +0.6 3,615,820 + +0.0 3,615,892 + nikE b3480 0.12 +0.0 3,615,892 + nikE b3480 0.12 +0.5 3,615,899 + nikE b3480 0.12 +0.4 3,615,966 + nikE b3480 0.21 -0.3 3,615,966 + nikE b3480 0.21 -1.1 3,615,966 + nikE b3480 0.21 -0.5 3,615,977 + nikE b3480 0.22 -0.7
Or see this region's nucleotide sequence