Experiment: LB Anaerobic with Irgasan 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpA and glpB overlap by 11 nucleotides glpB and glpC overlap by 4 nucleotides
b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297
glpA
b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546
glpB
b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733
glpC
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2351.298 kb on + strand, within glpA at 2351.353 kb on + strand, within glpA at 2351.353 kb on + strand, within glpA at 2351.392 kb on - strand, within glpA at 2351.608 kb on - strand, within glpA at 2351.686 kb on - strand, within glpA at 2351.686 kb on - strand, within glpA at 2351.773 kb on + strand, within glpA at 2351.773 kb on + strand, within glpA at 2351.792 kb on + strand, within glpA at 2351.792 kb on + strand, within glpA at 2351.801 kb on + strand, within glpA at 2351.801 kb on + strand, within glpA at 2351.802 kb on + strand, within glpA at 2351.802 kb on + strand, within glpA at 2351.809 kb on - strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.816 kb on + strand, within glpA at 2351.826 kb on + strand, within glpA at 2351.947 kb on + strand, within glpA at 2351.947 kb on + strand, within glpA at 2351.955 kb on - strand, within glpA at 2351.955 kb on - strand, within glpA at 2352.006 kb on - strand, within glpA at 2352.059 kb on + strand, within glpA at 2352.059 kb on + strand, within glpA at 2352.067 kb on - strand, within glpA at 2352.074 kb on - strand, within glpA at 2352.104 kb on + strand, within glpA at 2352.104 kb on + strand, within glpA at 2352.122 kb on + strand, within glpA at 2352.122 kb on + strand, within glpA at 2352.265 kb on + strand at 2352.300 kb on - strand at 2352.326 kb on + strand at 2352.347 kb on + strand at 2352.369 kb on + strand at 2352.440 kb on - strand, within glpB at 2352.447 kb on + strand, within glpB at 2352.461 kb on - strand, within glpB at 2352.463 kb on + strand, within glpB at 2352.463 kb on + strand, within glpB at 2352.506 kb on + strand, within glpB at 2352.506 kb on + strand, within glpB at 2352.547 kb on + strand, within glpB at 2352.631 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.787 kb on - strand, within glpB at 2353.281 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.379 kb on - strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.436 kb on + strand at 2353.436 kb on + strand at 2353.446 kb on + strand at 2353.575 kb on - strand at 2353.575 kb on - strand at 2353.577 kb on + strand at 2353.577 kb on + strand at 2353.615 kb on - strand at 2353.684 kb on + strand, within glpC at 2353.686 kb on + strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.767 kb on + strand, within glpC at 2353.812 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.871 kb on - strand, within glpC at 2354.115 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.169 kb on + strand, within glpC at 2354.278 kb on - strand, within glpC at 2354.438 kb on - strand, within glpC at 2354.534 kb on + strand, within glpC at 2354.542 kb on - strand, within glpC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Irgasan 0.000000625 mM remove 2,351,298 + glpA b2241 0.39 +0.4 2,351,353 + glpA b2241 0.42 -2.5 2,351,353 + glpA b2241 0.42 -1.9 2,351,392 - glpA b2241 0.44 -0.9 2,351,608 - glpA b2241 0.58 -0.9 2,351,686 - glpA b2241 0.62 -0.6 2,351,686 - glpA b2241 0.62 -1.4 2,351,773 + glpA b2241 0.68 -1.9 2,351,773 + glpA b2241 0.68 -0.1 2,351,792 + glpA b2241 0.69 -1.6 2,351,792 + glpA b2241 0.69 -2.3 2,351,801 + glpA b2241 0.69 -0.3 2,351,801 + glpA b2241 0.69 -2.0 2,351,802 + glpA b2241 0.70 -1.3 2,351,802 + glpA b2241 0.70 -0.8 2,351,809 - glpA b2241 0.70 -1.9 2,351,816 + glpA b2241 0.70 -0.9 2,351,816 + glpA b2241 0.70 +0.0 2,351,816 + glpA b2241 0.70 -2.6 2,351,816 + glpA b2241 0.70 -0.2 2,351,826 + glpA b2241 0.71 -1.7 2,351,947 + glpA b2241 0.78 -0.1 2,351,947 + glpA b2241 0.78 -1.1 2,351,955 - glpA b2241 0.79 -1.0 2,351,955 - glpA b2241 0.79 -1.3 2,352,006 - glpA b2241 0.82 -0.8 2,352,059 + glpA b2241 0.85 +0.7 2,352,059 + glpA b2241 0.85 -0.4 2,352,067 - glpA b2241 0.86 -0.5 2,352,074 - glpA b2241 0.86 -0.9 2,352,104 + glpA b2241 0.88 -0.9 2,352,104 + glpA b2241 0.88 -0.6 2,352,122 + glpA b2241 0.89 -3.1 2,352,122 + glpA b2241 0.89 -1.4 2,352,265 + -0.4 2,352,300 - -2.7 2,352,326 + -1.1 2,352,347 + -0.7 2,352,369 + -2.1 2,352,440 - glpB b2242 0.12 -1.0 2,352,447 + glpB b2242 0.13 -1.9 2,352,461 - glpB b2242 0.14 -1.3 2,352,463 + glpB b2242 0.14 -1.7 2,352,463 + glpB b2242 0.14 -3.0 2,352,506 + glpB b2242 0.17 -3.0 2,352,506 + glpB b2242 0.17 -0.6 2,352,547 + glpB b2242 0.21 -0.4 2,352,631 + glpB b2242 0.27 -1.6 2,352,650 + glpB b2242 0.29 -2.1 2,352,650 + glpB b2242 0.29 -0.6 2,352,787 - glpB b2242 0.40 -2.5 2,353,281 + glpB b2242 0.79 -2.3 2,353,291 + glpB b2242 0.80 -3.1 2,353,291 + glpB b2242 0.80 -0.6 2,353,326 - glpB b2242 0.82 -1.7 2,353,326 - glpB b2242 0.82 +0.4 2,353,379 - glpB b2242 0.87 -3.0 2,353,400 + glpB b2242 0.88 -2.4 2,353,400 + glpB b2242 0.88 +0.7 2,353,436 + -1.1 2,353,436 + -1.3 2,353,446 + +0.2 2,353,575 - +2.0 2,353,575 - -0.2 2,353,577 + -0.8 2,353,577 + -2.7 2,353,615 - -0.7 2,353,684 + glpC b2243 0.12 -0.9 2,353,686 + glpC b2243 0.12 -1.2 2,353,696 - glpC b2243 0.13 -0.1 2,353,696 - glpC b2243 0.13 -1.9 2,353,767 + glpC b2243 0.19 -1.6 2,353,812 + glpC b2243 0.23 -0.6 2,353,853 + glpC b2243 0.26 -0.9 2,353,853 + glpC b2243 0.26 -2.2 2,353,871 - glpC b2243 0.28 -3.1 2,354,115 - glpC b2243 0.48 -1.3 2,354,125 - glpC b2243 0.49 -0.1 2,354,125 - glpC b2243 0.49 -2.4 2,354,169 + glpC b2243 0.53 -2.2 2,354,278 - glpC b2243 0.62 -0.8 2,354,438 - glpC b2243 0.75 -1.5 2,354,534 + glpC b2243 0.83 -2.1 2,354,542 - glpC b2243 0.84 -0.6
Or see this region's nucleotide sequence