Strain Fitness in Escherichia coli BW25113 around b3833

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrmuC and ubiE are separated by 94 nucleotidesubiE and yigP are separated by 13 nucleotidesyigP and ubiB overlap by 4 nucleotides b3832: rmuC - predicted recombination limiting protein (NCBI), at 4,015,356 to 4,016,783 rmuC b3833: ubiE - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI), at 4,016,878 to 4,017,633 ubiE b3834: yigP - hypothetical protein (NCBI), at 4,017,647 to 4,018,252 yigP b3835: ubiB - putative ubiquinone biosynthesis protein UbiB (NCBI), at 4,018,249 to 4,019,889 ubiB Position (kb) 4016 4017 4018Strain fitness (log2 ratio) -2 -1 0 1 2at 4015.882 kb on + strand, within rmuCat 4015.882 kb on + strand, within rmuCat 4015.890 kb on - strand, within rmuCat 4015.922 kb on - strand, within rmuCat 4015.922 kb on - strand, within rmuCat 4015.933 kb on - strand, within rmuCat 4015.938 kb on + strand, within rmuCat 4015.956 kb on + strand, within rmuCat 4015.956 kb on + strand, within rmuCat 4015.964 kb on + strand, within rmuCat 4015.964 kb on + strand, within rmuCat 4015.975 kb on + strand, within rmuCat 4015.975 kb on + strand, within rmuCat 4015.988 kb on - strand, within rmuCat 4015.988 kb on - strand, within rmuCat 4015.989 kb on + strand, within rmuCat 4015.989 kb on + strand, within rmuCat 4015.992 kb on + strand, within rmuCat 4015.992 kb on + strand, within rmuCat 4016.010 kb on - strand, within rmuCat 4016.012 kb on + strand, within rmuCat 4016.012 kb on + strand, within rmuCat 4016.027 kb on - strand, within rmuCat 4016.027 kb on - strand, within rmuCat 4016.034 kb on + strand, within rmuCat 4016.034 kb on + strand, within rmuCat 4016.039 kb on + strand, within rmuCat 4016.039 kb on + strand, within rmuCat 4016.077 kb on - strand, within rmuCat 4016.082 kb on - strand, within rmuCat 4016.084 kb on + strand, within rmuCat 4016.202 kb on - strand, within rmuCat 4016.222 kb on - strand, within rmuCat 4016.294 kb on + strand, within rmuCat 4016.294 kb on + strand, within rmuCat 4016.309 kb on - strand, within rmuCat 4016.309 kb on - strand, within rmuCat 4016.338 kb on + strand, within rmuCat 4016.338 kb on + strand, within rmuCat 4016.343 kb on + strand, within rmuCat 4016.394 kb on + strand, within rmuCat 4016.394 kb on + strand, within rmuCat 4016.416 kb on + strand, within rmuCat 4016.419 kb on - strand, within rmuCat 4016.439 kb on + strand, within rmuCat 4016.439 kb on + strand, within rmuCat 4016.447 kb on - strand, within rmuCat 4016.563 kb on - strand, within rmuCat 4016.563 kb on - strand, within rmuCat 4016.632 kb on + strand, within rmuCat 4016.632 kb on + strand, within rmuCat 4016.632 kb on + strand, within rmuCat 4016.671 kb on + strandat 4016.671 kb on + strandat 4016.687 kb on + strandat 4016.687 kb on + strandat 4016.691 kb on - strandat 4016.699 kb on + strandat 4016.702 kb on + strandat 4016.712 kb on - strandat 4016.797 kb on + strandat 4016.797 kb on + strandat 4016.841 kb on - strandat 4016.841 kb on - strandat 4017.662 kb on + strandat 4017.674 kb on + strandat 4017.674 kb on + strandat 4017.698 kb on + strandat 4017.698 kb on + strandat 4017.711 kb on + strand, within yigPat 4017.732 kb on + strand, within yigPat 4017.752 kb on + strand, within yigPat 4017.797 kb on + strand, within yigPat 4017.797 kb on + strand, within yigPat 4017.816 kb on + strand, within yigPat 4017.816 kb on + strand, within yigPat 4017.843 kb on + strand, within yigPat 4017.843 kb on + strand, within yigPat 4017.916 kb on + strand, within yigPat 4017.992 kb on + strand, within yigPat 4017.992 kb on + strand, within yigPat 4018.018 kb on + strand, within yigPat 4018.018 kb on + strand, within yigPat 4018.038 kb on + strand, within yigP

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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4,015,882 + rmuC b3832 0.37 -0.3
4,015,882 + rmuC b3832 0.37 +0.6
4,015,890 - rmuC b3832 0.37 -0.8
4,015,922 - rmuC b3832 0.40 -0.2
4,015,922 - rmuC b3832 0.40 -1.0
4,015,933 - rmuC b3832 0.40 +0.4
4,015,938 + rmuC b3832 0.41 +0.1
4,015,956 + rmuC b3832 0.42 -0.6
4,015,956 + rmuC b3832 0.42 +0.4
4,015,964 + rmuC b3832 0.43 +0.6
4,015,964 + rmuC b3832 0.43 -2.2
4,015,975 + rmuC b3832 0.43 +0.3
4,015,975 + rmuC b3832 0.43 -0.8
4,015,988 - rmuC b3832 0.44 +0.8
4,015,988 - rmuC b3832 0.44 +0.3
4,015,989 + rmuC b3832 0.44 -0.9
4,015,989 + rmuC b3832 0.44 +0.3
4,015,992 + rmuC b3832 0.45 +1.2
4,015,992 + rmuC b3832 0.45 +0.2
4,016,010 - rmuC b3832 0.46 -0.1
4,016,012 + rmuC b3832 0.46 +0.3
4,016,012 + rmuC b3832 0.46 +0.1
4,016,027 - rmuC b3832 0.47 -1.2
4,016,027 - rmuC b3832 0.47 -1.8
4,016,034 + rmuC b3832 0.47 +0.4
4,016,034 + rmuC b3832 0.47 -0.7
4,016,039 + rmuC b3832 0.48 +0.6
4,016,039 + rmuC b3832 0.48 -0.3
4,016,077 - rmuC b3832 0.50 -0.5
4,016,082 - rmuC b3832 0.51 -1.0
4,016,084 + rmuC b3832 0.51 -0.4
4,016,202 - rmuC b3832 0.59 +0.5
4,016,222 - rmuC b3832 0.61 +0.4
4,016,294 + rmuC b3832 0.66 -0.6
4,016,294 + rmuC b3832 0.66 -0.3
4,016,309 - rmuC b3832 0.67 +0.2
4,016,309 - rmuC b3832 0.67 -0.8
4,016,338 + rmuC b3832 0.69 +0.4
4,016,338 + rmuC b3832 0.69 +0.4
4,016,343 + rmuC b3832 0.69 -0.7
4,016,394 + rmuC b3832 0.73 +0.6
4,016,394 + rmuC b3832 0.73 -1.8
4,016,416 + rmuC b3832 0.74 -0.7
4,016,419 - rmuC b3832 0.74 +0.1
4,016,439 + rmuC b3832 0.76 -0.2
4,016,439 + rmuC b3832 0.76 +1.6
4,016,447 - rmuC b3832 0.76 -0.2
4,016,563 - rmuC b3832 0.85 +0.5
4,016,563 - rmuC b3832 0.85 -0.1
4,016,632 + rmuC b3832 0.89 +0.2
4,016,632 + rmuC b3832 0.89 -0.7
4,016,632 + rmuC b3832 0.89 -1.2
4,016,671 + -0.2
4,016,671 + +0.5
4,016,687 + +1.0
4,016,687 + +0.4
4,016,691 - -1.6
4,016,699 + -0.3
4,016,702 + -0.5
4,016,712 - -0.2
4,016,797 + +0.6
4,016,797 + -0.2
4,016,841 - -0.4
4,016,841 - -0.4
4,017,662 + -0.2
4,017,674 + +1.3
4,017,674 + -0.3
4,017,698 + +0.9
4,017,698 + -0.1
4,017,711 + yigP b3834 0.11 +0.3
4,017,732 + yigP b3834 0.14 -0.2
4,017,752 + yigP b3834 0.17 -0.6
4,017,797 + yigP b3834 0.25 +1.2
4,017,797 + yigP b3834 0.25 +0.9
4,017,816 + yigP b3834 0.28 +0.8
4,017,816 + yigP b3834 0.28 +1.8
4,017,843 + yigP b3834 0.32 +0.1
4,017,843 + yigP b3834 0.32 -0.7
4,017,916 + yigP b3834 0.44 +0.2
4,017,992 + yigP b3834 0.57 +1.2
4,017,992 + yigP b3834 0.57 +0.9
4,018,018 + yigP b3834 0.61 -0.8
4,018,018 + yigP b3834 0.61 +1.6
4,018,038 + yigP b3834 0.65 +1.9

Or see this region's nucleotide sequence