Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnA and yieM are separated by 4 nucleotides yieM and yieN overlap by 7 nucleotides
b3744: asnA - asparagine synthetase AsnA (NCBI), at 3,925,178 to 3,926,170
asnA
b3745: yieM - predicted von Willibrand factor containing protein (NCBI), at 3,926,175 to 3,927,626
yieM
b3746: yieN - putative 2-component regulator (VIMSS), at 3,927,620 to 3,929,116
yieN
Position (kb)
3926
3927
3928 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3925.206 kb on + strand at 3925.206 kb on + strand at 3925.210 kb on + strand at 3925.254 kb on + strand at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.305 kb on - strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.348 kb on - strand, within asnA at 3925.358 kb on + strand, within asnA at 3925.388 kb on - strand, within asnA at 3925.411 kb on + strand, within asnA at 3925.417 kb on - strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.458 kb on - strand, within asnA at 3925.462 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA at 3925.489 kb on - strand, within asnA at 3925.520 kb on + strand, within asnA at 3925.536 kb on - strand, within asnA at 3925.543 kb on - strand, within asnA at 3925.592 kb on - strand, within asnA at 3925.592 kb on - strand, within asnA at 3925.606 kb on + strand, within asnA at 3925.607 kb on - strand, within asnA at 3925.632 kb on + strand, within asnA at 3925.632 kb on + strand, within asnA at 3925.637 kb on - strand, within asnA at 3925.644 kb on + strand, within asnA at 3925.675 kb on + strand, within asnA at 3925.675 kb on + strand, within asnA at 3925.684 kb on - strand, within asnA at 3925.686 kb on + strand, within asnA at 3925.692 kb on - strand, within asnA at 3925.693 kb on - strand, within asnA at 3925.789 kb on + strand, within asnA at 3925.797 kb on - strand, within asnA at 3925.808 kb on - strand, within asnA at 3925.808 kb on - strand, within asnA at 3925.823 kb on + strand, within asnA at 3925.842 kb on - strand, within asnA at 3925.845 kb on - strand at 3925.845 kb on - strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.853 kb on + strand, within asnA at 3925.853 kb on + strand, within asnA at 3925.860 kb on + strand, within asnA at 3925.868 kb on + strand, within asnA at 3925.868 kb on + strand, within asnA at 3925.941 kb on + strand, within asnA at 3925.954 kb on - strand, within asnA at 3925.986 kb on - strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.005 kb on + strand, within asnA at 3926.010 kb on + strand, within asnA at 3926.058 kb on + strand, within asnA at 3926.076 kb on + strand at 3926.080 kb on - strand at 3926.080 kb on - strand at 3926.093 kb on - strand at 3926.125 kb on + strand at 3926.127 kb on - strand at 3926.133 kb on - strand at 3926.135 kb on + strand at 3926.135 kb on + strand at 3926.148 kb on + strand at 3926.148 kb on + strand at 3926.165 kb on - strand at 3926.165 kb on - strand at 3926.261 kb on - strand at 3926.295 kb on + strand at 3926.308 kb on - strand at 3926.308 kb on - strand at 3926.308 kb on - strand at 3926.398 kb on + strand, within yieM at 3926.423 kb on - strand, within yieM at 3926.496 kb on + strand, within yieM at 3926.506 kb on - strand, within yieM at 3926.513 kb on + strand, within yieM at 3926.554 kb on - strand, within yieM at 3926.583 kb on + strand, within yieM at 3926.605 kb on + strand, within yieM at 3926.613 kb on - strand, within yieM at 3926.615 kb on + strand, within yieM at 3926.656 kb on + strand, within yieM at 3926.733 kb on - strand, within yieM at 3926.735 kb on - strand, within yieM at 3926.739 kb on + strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.801 kb on - strand, within yieM at 3926.807 kb on + strand, within yieM at 3926.807 kb on + strand, within yieM at 3926.839 kb on - strand, within yieM at 3926.874 kb on - strand, within yieM at 3926.874 kb on - strand, within yieM at 3926.927 kb on - strand, within yieM at 3926.942 kb on + strand, within yieM at 3926.942 kb on + strand, within yieM at 3927.009 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.070 kb on + strand, within yieM at 3927.070 kb on + strand, within yieM at 3927.071 kb on + strand, within yieM at 3927.071 kb on + strand, within yieM at 3927.104 kb on - strand, within yieM at 3927.104 kb on - strand, within yieM at 3927.129 kb on + strand, within yieM at 3927.154 kb on - strand, within yieM at 3927.157 kb on + strand, within yieM at 3927.157 kb on + strand, within yieM at 3927.159 kb on + strand, within yieM at 3927.167 kb on - strand, within yieM at 3927.180 kb on + strand, within yieM at 3927.199 kb on - strand, within yieM at 3927.260 kb on - strand, within yieM at 3927.260 kb on - strand, within yieM at 3927.291 kb on + strand, within yieM at 3927.291 kb on + strand, within yieM at 3927.297 kb on + strand, within yieM at 3927.297 kb on + strand, within yieM at 3927.305 kb on - strand, within yieM at 3927.315 kb on - strand, within yieM at 3927.329 kb on + strand, within yieM at 3927.336 kb on + strand, within yieM at 3927.336 kb on + strand, within yieM at 3927.344 kb on - strand, within yieM at 3927.359 kb on + strand, within yieM at 3927.380 kb on + strand, within yieM at 3927.392 kb on + strand, within yieM at 3927.410 kb on - strand, within yieM at 3927.417 kb on - strand, within yieM at 3927.417 kb on - strand, within yieM at 3927.427 kb on - strand, within yieM at 3927.427 kb on - strand, within yieM at 3927.427 kb on - strand, within yieM at 3927.456 kb on - strand, within yieM at 3927.543 kb on + strand at 3927.543 kb on + strand at 3927.543 kb on + strand at 3927.551 kb on + strand at 3927.551 kb on + strand at 3927.551 kb on + strand at 3927.551 kb on - strand at 3927.551 kb on - strand at 3927.551 kb on - strand at 3927.587 kb on - strand at 3927.589 kb on - strand at 3927.610 kb on - strand at 3927.631 kb on - strand at 3927.636 kb on - strand at 3927.636 kb on - strand at 3927.653 kb on + strand at 3927.653 kb on + strand at 3927.654 kb on + strand at 3927.666 kb on - strand at 3927.696 kb on + strand at 3927.725 kb on + strand at 3927.738 kb on + strand at 3927.771 kb on - strand, within yieN at 3927.791 kb on - strand, within yieN at 3927.802 kb on - strand, within yieN at 3927.803 kb on - strand, within yieN at 3927.820 kb on + strand, within yieN at 3927.820 kb on + strand, within yieN at 3927.825 kb on - strand, within yieN at 3927.838 kb on - strand, within yieN at 3927.855 kb on + strand, within yieN at 3927.908 kb on + strand, within yieN at 3927.908 kb on + strand, within yieN at 3927.909 kb on - strand, within yieN at 3927.909 kb on - strand, within yieN at 3927.919 kb on - strand, within yieN at 3927.919 kb on - strand, within yieN at 3927.919 kb on - strand, within yieN at 3927.946 kb on + strand, within yieN at 3927.946 kb on + strand, within yieN at 3927.948 kb on + strand, within yieN at 3927.967 kb on + strand, within yieN at 3927.975 kb on - strand, within yieN at 3927.985 kb on - strand, within yieN at 3927.996 kb on - strand, within yieN at 3928.033 kb on + strand, within yieN at 3928.035 kb on - strand, within yieN at 3928.035 kb on - strand, within yieN at 3928.069 kb on + strand, within yieN at 3928.069 kb on + strand, within yieN at 3928.127 kb on + strand, within yieN at 3928.127 kb on + strand, within yieN at 3928.177 kb on + strand, within yieN at 3928.177 kb on + strand, within yieN at 3928.268 kb on - strand, within yieN at 3928.275 kb on - strand, within yieN at 3928.275 kb on - strand, within yieN at 3928.286 kb on - strand, within yieN at 3928.291 kb on + strand, within yieN at 3928.305 kb on - strand, within yieN at 3928.305 kb on - strand, within yieN at 3928.305 kb on - strand, within yieN at 3928.336 kb on + strand, within yieN at 3928.336 kb on + strand, within yieN at 3928.344 kb on - strand, within yieN at 3928.375 kb on + strand, within yieN at 3928.383 kb on - strand, within yieN at 3928.383 kb on - strand, within yieN at 3928.400 kb on - strand, within yieN at 3928.400 kb on - strand, within yieN at 3928.442 kb on - strand, within yieN at 3928.484 kb on + strand, within yieN at 3928.484 kb on - strand, within yieN at 3928.491 kb on + strand, within yieN at 3928.507 kb on - strand, within yieN at 3928.544 kb on + strand, within yieN at 3928.544 kb on + strand, within yieN at 3928.552 kb on - strand, within yieN at 3928.552 kb on - strand, within yieN at 3928.552 kb on - strand, within yieN at 3928.557 kb on - strand, within yieN at 3928.566 kb on + strand, within yieN at 3928.566 kb on + strand, within yieN at 3928.581 kb on + strand, within yieN at 3928.608 kb on + strand, within yieN at 3928.608 kb on + strand, within yieN at 3928.618 kb on - strand, within yieN at 3928.618 kb on - strand, within yieN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 3,925,206 + -0.7 3,925,206 + +0.7 3,925,210 + +0.5 3,925,254 + -0.7 3,925,301 - asnA b3744 0.12 +1.8 3,925,301 - asnA b3744 0.12 +0.1 3,925,301 - asnA b3744 0.12 -0.5 3,925,305 - asnA b3744 0.13 +1.2 3,925,312 + asnA b3744 0.13 -0.6 3,925,312 + asnA b3744 0.13 +0.5 3,925,312 + asnA b3744 0.13 +0.4 3,925,320 - asnA b3744 0.14 +0.5 3,925,320 - asnA b3744 0.14 +0.8 3,925,320 - asnA b3744 0.14 +0.6 3,925,348 - asnA b3744 0.17 +0.7 3,925,358 + asnA b3744 0.18 -0.8 3,925,388 - asnA b3744 0.21 -1.3 3,925,411 + asnA b3744 0.23 -1.4 3,925,417 - asnA b3744 0.24 +0.0 3,925,443 + asnA b3744 0.27 +1.5 3,925,443 + asnA b3744 0.27 -1.8 3,925,458 - asnA b3744 0.28 -0.2 3,925,462 - asnA b3744 0.29 -1.9 3,925,470 - asnA b3744 0.29 -0.5 3,925,470 - asnA b3744 0.29 +0.9 3,925,489 - asnA b3744 0.31 -0.3 3,925,520 + asnA b3744 0.34 +0.8 3,925,536 - asnA b3744 0.36 +0.1 3,925,543 - asnA b3744 0.37 +0.9 3,925,592 - asnA b3744 0.42 -0.9 3,925,592 - asnA b3744 0.42 +0.6 3,925,606 + asnA b3744 0.43 +0.6 3,925,607 - asnA b3744 0.43 -0.6 3,925,632 + asnA b3744 0.46 -0.5 3,925,632 + asnA b3744 0.46 +1.4 3,925,637 - asnA b3744 0.46 -0.4 3,925,644 + asnA b3744 0.47 +0.7 3,925,675 + asnA b3744 0.50 +0.6 3,925,675 + asnA b3744 0.50 +0.7 3,925,684 - asnA b3744 0.51 -0.1 3,925,686 + asnA b3744 0.51 +0.5 3,925,692 - asnA b3744 0.52 -1.2 3,925,693 - asnA b3744 0.52 +0.6 3,925,789 + asnA b3744 0.62 -1.1 3,925,797 - asnA b3744 0.62 -0.4 3,925,808 - asnA b3744 0.63 +0.1 3,925,808 - asnA b3744 0.63 -0.4 3,925,823 + asnA b3744 0.65 +0.5 3,925,842 - asnA b3744 0.67 +0.5 3,925,845 - +0.2 3,925,845 - asnA b3744 0.67 +0.6 3,925,848 + asnA b3744 0.67 +0.5 3,925,848 + asnA b3744 0.67 -0.4 3,925,848 + asnA b3744 0.67 +1.2 3,925,848 + asnA b3744 0.67 -0.1 3,925,853 + asnA b3744 0.68 -0.0 3,925,853 + asnA b3744 0.68 -0.1 3,925,860 + asnA b3744 0.69 +0.4 3,925,868 + asnA b3744 0.69 +1.1 3,925,868 + asnA b3744 0.69 -1.8 3,925,941 + asnA b3744 0.77 -0.5 3,925,954 - asnA b3744 0.78 +0.6 3,925,986 - asnA b3744 0.81 +1.1 3,926,000 + asnA b3744 0.83 +0.1 3,926,000 + asnA b3744 0.83 -0.8 3,926,000 + asnA b3744 0.83 +0.4 3,926,005 + asnA b3744 0.83 +0.5 3,926,010 + asnA b3744 0.84 +0.5 3,926,058 + asnA b3744 0.89 -0.0 3,926,076 + +0.2 3,926,080 - +0.0 3,926,080 - -0.2 3,926,093 - -1.7 3,926,125 + -0.4 3,926,127 - +0.5 3,926,133 - +0.2 3,926,135 + -0.8 3,926,135 + -0.4 3,926,148 + -0.4 3,926,148 + -1.5 3,926,165 - -0.4 3,926,165 - -1.9 3,926,261 - +0.8 3,926,295 + +2.2 3,926,308 - +1.8 3,926,308 - -1.1 3,926,308 - -0.1 3,926,398 + yieM b3745 0.15 +1.1 3,926,423 - yieM b3745 0.17 +0.9 3,926,496 + yieM b3745 0.22 +1.4 3,926,506 - yieM b3745 0.23 +1.3 3,926,513 + yieM b3745 0.23 +2.3 3,926,554 - yieM b3745 0.26 +1.4 3,926,583 + yieM b3745 0.28 -0.1 3,926,605 + yieM b3745 0.30 +1.9 3,926,613 - yieM b3745 0.30 +0.6 3,926,615 + yieM b3745 0.30 +1.3 3,926,656 + yieM b3745 0.33 +1.4 3,926,733 - yieM b3745 0.38 +0.5 3,926,735 - yieM b3745 0.39 +1.4 3,926,739 + yieM b3745 0.39 +1.6 3,926,752 - yieM b3745 0.40 +1.1 3,926,752 - yieM b3745 0.40 +1.1 3,926,752 - yieM b3745 0.40 +0.5 3,926,752 - yieM b3745 0.40 +1.2 3,926,752 - yieM b3745 0.40 +1.2 3,926,752 - yieM b3745 0.40 +1.9 3,926,752 - yieM b3745 0.40 +0.1 3,926,801 - yieM b3745 0.43 +2.0 3,926,807 + yieM b3745 0.44 +1.0 3,926,807 + yieM b3745 0.44 +1.2 3,926,839 - yieM b3745 0.46 +1.5 3,926,874 - yieM b3745 0.48 +2.0 3,926,874 - yieM b3745 0.48 +1.4 3,926,927 - yieM b3745 0.52 +0.7 3,926,942 + yieM b3745 0.53 +0.6 3,926,942 + yieM b3745 0.53 +0.7 3,927,009 + yieM b3745 0.57 +1.8 3,927,024 + yieM b3745 0.58 +0.2 3,927,024 + yieM b3745 0.58 +0.4 3,927,024 + yieM b3745 0.58 +1.4 3,927,070 + yieM b3745 0.62 +0.7 3,927,070 + yieM b3745 0.62 +1.6 3,927,071 + yieM b3745 0.62 +0.9 3,927,071 + yieM b3745 0.62 +1.0 3,927,104 - yieM b3745 0.64 +0.8 3,927,104 - yieM b3745 0.64 +0.9 3,927,129 + yieM b3745 0.66 +0.8 3,927,154 - yieM b3745 0.67 +2.0 3,927,157 + yieM b3745 0.68 +0.7 3,927,157 + yieM b3745 0.68 +1.1 3,927,159 + yieM b3745 0.68 +1.4 3,927,167 - yieM b3745 0.68 +1.3 3,927,180 + yieM b3745 0.69 +1.9 3,927,199 - yieM b3745 0.71 +1.2 3,927,260 - yieM b3745 0.75 +1.1 3,927,260 - yieM b3745 0.75 +1.0 3,927,291 + yieM b3745 0.77 +0.6 3,927,291 + yieM b3745 0.77 +2.0 3,927,297 + yieM b3745 0.77 +0.9 3,927,297 + yieM b3745 0.77 +0.0 3,927,305 - yieM b3745 0.78 +0.2 3,927,315 - yieM b3745 0.79 +1.2 3,927,329 + yieM b3745 0.79 +0.7 3,927,336 + yieM b3745 0.80 +1.1 3,927,336 + yieM b3745 0.80 +1.3 3,927,344 - yieM b3745 0.81 +0.2 3,927,359 + yieM b3745 0.82 +0.2 3,927,380 + yieM b3745 0.83 +0.4 3,927,392 + yieM b3745 0.84 +1.7 3,927,410 - yieM b3745 0.85 +0.8 3,927,417 - yieM b3745 0.86 +1.4 3,927,417 - yieM b3745 0.86 +0.6 3,927,427 - yieM b3745 0.86 +0.6 3,927,427 - yieM b3745 0.86 +1.7 3,927,427 - yieM b3745 0.86 +2.2 3,927,456 - yieM b3745 0.88 +1.2 3,927,543 + +1.0 3,927,543 + +0.6 3,927,543 + +1.2 3,927,551 + +0.9 3,927,551 + +1.0 3,927,551 + +1.0 3,927,551 - +1.7 3,927,551 - +1.6 3,927,551 - +0.6 3,927,587 - +1.2 3,927,589 - +1.0 3,927,610 - +1.2 3,927,631 - -0.6 3,927,636 - -1.8 3,927,636 - -0.4 3,927,653 + +0.6 3,927,653 + +1.6 3,927,654 + +1.6 3,927,666 - +0.9 3,927,696 + +0.4 3,927,725 + +1.1 3,927,738 + +1.4 3,927,771 - yieN b3746 0.10 +0.6 3,927,791 - yieN b3746 0.11 +2.0 3,927,802 - yieN b3746 0.12 +1.4 3,927,803 - yieN b3746 0.12 +1.3 3,927,820 + yieN b3746 0.13 +0.9 3,927,820 + yieN b3746 0.13 +0.9 3,927,825 - yieN b3746 0.14 +0.4 3,927,838 - yieN b3746 0.15 +2.3 3,927,855 + yieN b3746 0.16 +0.9 3,927,908 + yieN b3746 0.19 +0.8 3,927,908 + yieN b3746 0.19 +0.3 3,927,909 - yieN b3746 0.19 -0.3 3,927,909 - yieN b3746 0.19 +2.7 3,927,919 - yieN b3746 0.20 +0.5 3,927,919 - yieN b3746 0.20 +1.3 3,927,919 - yieN b3746 0.20 +1.6 3,927,946 + yieN b3746 0.22 +1.8 3,927,946 + yieN b3746 0.22 +1.0 3,927,948 + yieN b3746 0.22 +0.6 3,927,967 + yieN b3746 0.23 +2.0 3,927,975 - yieN b3746 0.24 +0.5 3,927,985 - yieN b3746 0.24 +1.4 3,927,996 - yieN b3746 0.25 +1.2 3,928,033 + yieN b3746 0.28 +0.6 3,928,035 - yieN b3746 0.28 +2.4 3,928,035 - yieN b3746 0.28 -0.8 3,928,069 + yieN b3746 0.30 +0.6 3,928,069 + yieN b3746 0.30 +1.2 3,928,127 + yieN b3746 0.34 +1.7 3,928,127 + yieN b3746 0.34 +1.6 3,928,177 + yieN b3746 0.37 +1.2 3,928,177 + yieN b3746 0.37 +0.5 3,928,268 - yieN b3746 0.43 -0.4 3,928,275 - yieN b3746 0.44 +0.5 3,928,275 - yieN b3746 0.44 +1.1 3,928,286 - yieN b3746 0.44 +1.9 3,928,291 + yieN b3746 0.45 +0.0 3,928,305 - yieN b3746 0.46 +0.2 3,928,305 - yieN b3746 0.46 +0.1 3,928,305 - yieN b3746 0.46 +1.4 3,928,336 + yieN b3746 0.48 +1.7 3,928,336 + yieN b3746 0.48 -0.5 3,928,344 - yieN b3746 0.48 +1.1 3,928,375 + yieN b3746 0.50 +1.6 3,928,383 - yieN b3746 0.51 +1.3 3,928,383 - yieN b3746 0.51 +0.5 3,928,400 - yieN b3746 0.52 +1.8 3,928,400 - yieN b3746 0.52 +0.7 3,928,442 - yieN b3746 0.55 +0.8 3,928,484 + yieN b3746 0.58 +0.9 3,928,484 - yieN b3746 0.58 +0.9 3,928,491 + yieN b3746 0.58 +0.3 3,928,507 - yieN b3746 0.59 +1.1 3,928,544 + yieN b3746 0.62 +1.7 3,928,544 + yieN b3746 0.62 +1.1 3,928,552 - yieN b3746 0.62 +1.7 3,928,552 - yieN b3746 0.62 +1.2 3,928,552 - yieN b3746 0.62 +2.4 3,928,557 - yieN b3746 0.63 +1.1 3,928,566 + yieN b3746 0.63 +0.8 3,928,566 + yieN b3746 0.63 +0.7 3,928,581 + yieN b3746 0.64 +0.4 3,928,608 + yieN b3746 0.66 +0.4 3,928,608 + yieN b3746 0.66 +1.0 3,928,618 - yieN b3746 0.67 -0.1 3,928,618 - yieN b3746 0.67 +1.0
Or see this region's nucleotide sequence