Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt otsA and otsB overlap by 26 nucleotides otsB and araH are separated by 166 nucleotides araH and araG are separated by 14 nucleotides
b1896: otsA - trehalose-6-phosphate synthase (NCBI), at 1,978,212 to 1,979,636
otsA
b1897: otsB - trehalose-6-phosphate phosphatase, biosynthetic (NCBI), at 1,979,611 to 1,980,411
otsB
b4460: araH - fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq), at 1,980,578 to 1,981,564
araH
b1900: araG - fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,981,579 to 1,983,093
araG
Position (kb)
1980
1981
1982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1979.748 kb on - strand, within otsB at 1979.885 kb on + strand, within otsB at 1979.998 kb on - strand, within otsB at 1980.189 kb on - strand, within otsB at 1980.229 kb on - strand, within otsB at 1980.256 kb on - strand, within otsB at 1980.274 kb on + strand, within otsB at 1980.341 kb on + strand at 1980.827 kb on - strand at 1980.984 kb on + strand, within araH at 1981.002 kb on - strand, within araH at 1981.002 kb on - strand, within araH at 1981.480 kb on + strand at 1981.487 kb on + strand at 1981.505 kb on - strand at 1981.531 kb on - strand at 1981.800 kb on + strand, within araG at 1982.173 kb on - strand, within araG at 1982.173 kb on - strand, within araG at 1982.180 kb on + strand, within araG at 1982.406 kb on + strand, within araG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 1,979,748 - otsB b1897 0.17 +0.6 1,979,885 + otsB b1897 0.34 -0.3 1,979,998 - otsB b1897 0.48 +0.8 1,980,189 - otsB b1897 0.72 -0.4 1,980,229 - otsB b1897 0.77 -0.3 1,980,256 - otsB b1897 0.81 +1.6 1,980,274 + otsB b1897 0.83 +0.3 1,980,341 + +1.2 1,980,827 - -0.8 1,980,984 + araH b4460 0.41 -0.7 1,981,002 - araH b4460 0.43 -0.9 1,981,002 - araH b4460 0.43 -2.0 1,981,480 + -2.7 1,981,487 + -0.0 1,981,505 - +0.1 1,981,531 - -0.8 1,981,800 + araG b1900 0.15 +0.2 1,982,173 - araG b1900 0.39 -0.7 1,982,173 - araG b1900 0.39 +0.2 1,982,180 + araG b1900 0.40 +2.3 1,982,406 + araG b1900 0.55 -0.5
Or see this region's nucleotide sequence