Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdh and kbl are separated by 9 nucleotides kbl and htrL are separated by 274 nucleotides
b3616: tdh - L-threonine 3-dehydrogenase (NCBI), at 3,788,343 to 3,789,368
tdh
b3617: kbl - 2-amino-3-ketobutyrate coenzyme A ligase (NCBI), at 3,789,378 to 3,790,574
kbl
b3618: htrL - involved in lipopolysaccharide biosynthesis (VIMSS), at 3,790,849 to 3,791,706
htrL
Position (kb)
3789
3790
3791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3788.378 kb on - strand at 3788.378 kb on - strand at 3788.378 kb on - strand at 3788.383 kb on - strand at 3788.383 kb on - strand at 3788.389 kb on - strand at 3788.389 kb on - strand at 3788.392 kb on - strand at 3788.397 kb on - strand at 3788.398 kb on - strand at 3788.398 kb on - strand at 3788.399 kb on - strand at 3788.427 kb on - strand at 3788.427 kb on - strand at 3788.471 kb on + strand, within tdh at 3788.471 kb on + strand, within tdh at 3788.506 kb on - strand, within tdh at 3788.561 kb on + strand, within tdh at 3788.569 kb on - strand, within tdh at 3788.580 kb on + strand, within tdh at 3788.586 kb on - strand, within tdh at 3788.609 kb on - strand, within tdh at 3788.624 kb on - strand, within tdh at 3788.652 kb on - strand, within tdh at 3788.681 kb on - strand, within tdh at 3788.693 kb on - strand, within tdh at 3788.725 kb on + strand, within tdh at 3788.761 kb on + strand, within tdh at 3788.813 kb on + strand, within tdh at 3788.914 kb on + strand, within tdh at 3788.974 kb on + strand, within tdh at 3789.042 kb on - strand, within tdh at 3789.086 kb on + strand, within tdh at 3789.086 kb on + strand, within tdh at 3789.149 kb on + strand, within tdh at 3789.149 kb on + strand, within tdh at 3789.164 kb on + strand, within tdh at 3789.228 kb on - strand, within tdh at 3789.229 kb on + strand, within tdh at 3789.229 kb on + strand, within tdh at 3789.238 kb on - strand, within tdh at 3789.250 kb on + strand, within tdh at 3789.263 kb on - strand, within tdh at 3789.290 kb on + strand at 3789.300 kb on - strand at 3789.345 kb on + strand at 3789.374 kb on - strand at 3789.447 kb on + strand at 3789.447 kb on + strand at 3789.510 kb on - strand, within kbl at 3789.565 kb on + strand, within kbl at 3789.582 kb on - strand, within kbl at 3789.590 kb on + strand, within kbl at 3789.607 kb on + strand, within kbl at 3789.607 kb on + strand, within kbl at 3789.607 kb on + strand, within kbl at 3789.675 kb on + strand, within kbl at 3789.715 kb on - strand, within kbl at 3789.836 kb on - strand, within kbl at 3789.883 kb on - strand, within kbl at 3789.903 kb on + strand, within kbl at 3789.903 kb on + strand, within kbl at 3789.944 kb on + strand, within kbl at 3789.949 kb on + strand, within kbl at 3789.950 kb on - strand, within kbl at 3789.950 kb on - strand, within kbl at 3789.952 kb on - strand, within kbl at 3789.952 kb on - strand, within kbl at 3789.991 kb on + strand, within kbl at 3789.991 kb on + strand, within kbl at 3790.023 kb on + strand, within kbl at 3790.133 kb on - strand, within kbl at 3790.150 kb on - strand, within kbl at 3790.178 kb on - strand, within kbl at 3790.236 kb on - strand, within kbl at 3790.257 kb on - strand, within kbl at 3790.257 kb on - strand, within kbl at 3790.274 kb on - strand, within kbl at 3790.274 kb on - strand, within kbl at 3790.304 kb on + strand, within kbl at 3790.314 kb on + strand, within kbl at 3790.314 kb on + strand, within kbl at 3790.327 kb on + strand, within kbl at 3790.328 kb on + strand, within kbl at 3790.357 kb on - strand, within kbl at 3790.357 kb on - strand, within kbl at 3790.402 kb on - strand, within kbl at 3790.402 kb on - strand, within kbl at 3790.456 kb on - strand at 3790.459 kb on + strand at 3790.460 kb on - strand at 3790.460 kb on - strand at 3790.463 kb on + strand at 3790.463 kb on - strand at 3790.471 kb on - strand at 3790.473 kb on + strand at 3790.486 kb on - strand at 3790.486 kb on - strand at 3790.531 kb on + strand at 3790.531 kb on + strand at 3790.531 kb on + strand at 3790.531 kb on + strand at 3790.539 kb on - strand at 3790.539 kb on - strand at 3790.539 kb on - strand at 3790.539 kb on - strand at 3790.539 kb on - strand at 3790.539 kb on - strand at 3790.559 kb on - strand at 3790.581 kb on + strand at 3790.581 kb on + strand at 3790.581 kb on + strand at 3790.581 kb on + strand at 3790.582 kb on + strand at 3790.588 kb on + strand at 3790.589 kb on - strand at 3790.589 kb on - strand at 3790.617 kb on + strand at 3790.617 kb on + strand at 3790.625 kb on - strand at 3790.625 kb on - strand at 3790.641 kb on + strand at 3790.643 kb on + strand at 3790.643 kb on - strand at 3790.643 kb on - strand at 3790.691 kb on - strand at 3790.698 kb on + strand at 3790.698 kb on - strand at 3790.698 kb on - strand at 3790.807 kb on + strand at 3790.812 kb on + strand at 3790.824 kb on - strand at 3790.840 kb on - strand at 3790.844 kb on - strand at 3790.890 kb on + strand at 3790.898 kb on + strand at 3790.898 kb on + strand at 3790.903 kb on + strand at 3790.904 kb on + strand at 3790.906 kb on - strand at 3790.906 kb on - strand at 3790.912 kb on - strand at 3790.913 kb on - strand at 3790.920 kb on - strand at 3790.920 kb on - strand at 3790.945 kb on - strand, within htrL at 3790.950 kb on + strand, within htrL at 3790.950 kb on + strand, within htrL at 3790.954 kb on - strand, within htrL at 3790.954 kb on - strand, within htrL at 3790.955 kb on + strand, within htrL at 3790.955 kb on + strand, within htrL at 3790.955 kb on + strand, within htrL at 3790.957 kb on + strand, within htrL at 3790.957 kb on + strand, within htrL at 3790.959 kb on - strand, within htrL at 3790.960 kb on + strand, within htrL at 3790.960 kb on + strand, within htrL at 3790.963 kb on - strand, within htrL at 3790.963 kb on - strand, within htrL at 3790.963 kb on - strand, within htrL at 3790.965 kb on - strand, within htrL at 3790.968 kb on - strand, within htrL at 3790.968 kb on - strand, within htrL at 3790.984 kb on - strand, within htrL at 3790.987 kb on + strand, within htrL at 3790.992 kb on - strand, within htrL at 3790.992 kb on - strand, within htrL at 3791.016 kb on - strand, within htrL at 3791.016 kb on - strand, within htrL at 3791.156 kb on - strand, within htrL at 3791.156 kb on - strand, within htrL at 3791.164 kb on - strand, within htrL at 3791.183 kb on - strand, within htrL at 3791.191 kb on - strand, within htrL at 3791.191 kb on - strand, within htrL at 3791.193 kb on - strand, within htrL at 3791.203 kb on - strand, within htrL at 3791.246 kb on - strand, within htrL at 3791.247 kb on - strand, within htrL at 3791.270 kb on - strand, within htrL at 3791.271 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.316 kb on - strand, within htrL at 3791.316 kb on - strand, within htrL at 3791.347 kb on - strand, within htrL at 3791.359 kb on - strand, within htrL at 3791.360 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.419 kb on - strand, within htrL at 3791.571 kb on - strand, within htrL at 3791.571 kb on - strand, within htrL
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 3,788,378 - -2.7 3,788,378 - -1.4 3,788,378 - -2.1 3,788,383 - -2.3 3,788,383 - +0.3 3,788,389 - -0.9 3,788,389 - -1.1 3,788,392 - -0.9 3,788,397 - -0.7 3,788,398 - -1.3 3,788,398 - -2.3 3,788,399 - -2.3 3,788,427 - -0.7 3,788,427 - +2.3 3,788,471 + tdh b3616 0.12 -2.1 3,788,471 + tdh b3616 0.12 -0.7 3,788,506 - tdh b3616 0.16 -1.3 3,788,561 + tdh b3616 0.21 +1.3 3,788,569 - tdh b3616 0.22 -1.3 3,788,580 + tdh b3616 0.23 -1.0 3,788,586 - tdh b3616 0.24 +0.8 3,788,609 - tdh b3616 0.26 -0.7 3,788,624 - tdh b3616 0.27 -0.7 3,788,652 - tdh b3616 0.30 -1.0 3,788,681 - tdh b3616 0.33 -2.2 3,788,693 - tdh b3616 0.34 -1.1 3,788,725 + tdh b3616 0.37 -1.3 3,788,761 + tdh b3616 0.41 +1.3 3,788,813 + tdh b3616 0.46 -2.4 3,788,914 + tdh b3616 0.56 -2.1 3,788,974 + tdh b3616 0.62 +0.3 3,789,042 - tdh b3616 0.68 -3.4 3,789,086 + tdh b3616 0.72 -0.3 3,789,086 + tdh b3616 0.72 +2.3 3,789,149 + tdh b3616 0.79 -0.2 3,789,149 + tdh b3616 0.79 -0.1 3,789,164 + tdh b3616 0.80 +0.0 3,789,228 - tdh b3616 0.86 -0.7 3,789,229 + tdh b3616 0.86 -1.1 3,789,229 + tdh b3616 0.86 -0.7 3,789,238 - tdh b3616 0.87 -2.8 3,789,250 + tdh b3616 0.88 -0.1 3,789,263 - tdh b3616 0.90 -1.5 3,789,290 + -1.5 3,789,300 - -2.1 3,789,345 + -0.3 3,789,374 - +0.3 3,789,447 + +0.0 3,789,447 + -1.5 3,789,510 - kbl b3617 0.11 -1.5 3,789,565 + kbl b3617 0.16 +0.1 3,789,582 - kbl b3617 0.17 -1.8 3,789,590 + kbl b3617 0.18 +1.3 3,789,607 + kbl b3617 0.19 -0.7 3,789,607 + kbl b3617 0.19 -2.5 3,789,607 + kbl b3617 0.19 -0.1 3,789,675 + kbl b3617 0.25 -0.3 3,789,715 - kbl b3617 0.28 -1.2 3,789,836 - kbl b3617 0.38 -0.3 3,789,883 - kbl b3617 0.42 -3.5 3,789,903 + kbl b3617 0.44 -2.2 3,789,903 + kbl b3617 0.44 -1.1 3,789,944 + kbl b3617 0.47 -1.1 3,789,949 + kbl b3617 0.48 -1.5 3,789,950 - kbl b3617 0.48 -1.7 3,789,950 - kbl b3617 0.48 -1.1 3,789,952 - kbl b3617 0.48 -2.3 3,789,952 - kbl b3617 0.48 +1.3 3,789,991 + kbl b3617 0.51 -1.1 3,789,991 + kbl b3617 0.51 -2.2 3,790,023 + kbl b3617 0.54 -1.1 3,790,133 - kbl b3617 0.63 -1.7 3,790,150 - kbl b3617 0.64 -2.1 3,790,178 - kbl b3617 0.67 -2.8 3,790,236 - kbl b3617 0.72 -3.2 3,790,257 - kbl b3617 0.73 +1.3 3,790,257 - kbl b3617 0.73 -0.3 3,790,274 - kbl b3617 0.75 -2.7 3,790,274 - kbl b3617 0.75 -2.2 3,790,304 + kbl b3617 0.77 -1.9 3,790,314 + kbl b3617 0.78 -1.5 3,790,314 + kbl b3617 0.78 +0.3 3,790,327 + kbl b3617 0.79 -1.2 3,790,328 + kbl b3617 0.79 -1.5 3,790,357 - kbl b3617 0.82 -0.7 3,790,357 - kbl b3617 0.82 -0.5 3,790,402 - kbl b3617 0.86 -0.9 3,790,402 - kbl b3617 0.86 -0.7 3,790,456 - -0.7 3,790,459 + +0.3 3,790,460 - +1.3 3,790,460 - +2.6 3,790,463 + -1.1 3,790,463 - -1.7 3,790,471 - -0.1 3,790,473 + +0.3 3,790,486 - +1.3 3,790,486 - -2.6 3,790,531 + +0.3 3,790,531 + -1.7 3,790,531 + -0.3 3,790,531 + -1.1 3,790,539 - -2.4 3,790,539 - -1.7 3,790,539 - -1.5 3,790,539 - -2.1 3,790,539 - -1.1 3,790,539 - -2.1 3,790,559 - -0.5 3,790,581 + -0.7 3,790,581 + -0.9 3,790,581 + -1.6 3,790,581 + -1.9 3,790,582 + -2.6 3,790,588 + -0.3 3,790,589 - -2.3 3,790,589 - -1.2 3,790,617 + +0.3 3,790,617 + -1.1 3,790,625 - -0.4 3,790,625 - +0.0 3,790,641 + -1.3 3,790,643 + -1.0 3,790,643 - -1.1 3,790,643 - +1.3 3,790,691 - +1.0 3,790,698 + -0.7 3,790,698 - +0.8 3,790,698 - +1.0 3,790,807 + +0.5 3,790,812 + +0.3 3,790,824 - +1.1 3,790,840 - -0.7 3,790,844 - -0.7 3,790,890 + -2.4 3,790,898 + +0.3 3,790,898 + -1.1 3,790,903 + +0.7 3,790,904 + +0.7 3,790,906 - +1.0 3,790,906 - +1.3 3,790,912 - +0.4 3,790,913 - +0.4 3,790,920 - -2.1 3,790,920 - -1.3 3,790,945 - htrL b3618 0.11 +0.4 3,790,950 + htrL b3618 0.12 -1.0 3,790,950 + htrL b3618 0.12 -0.3 3,790,954 - htrL b3618 0.12 -2.0 3,790,954 - htrL b3618 0.12 +0.6 3,790,955 + htrL b3618 0.12 +0.6 3,790,955 + htrL b3618 0.12 -0.1 3,790,955 + htrL b3618 0.12 -0.8 3,790,957 + htrL b3618 0.13 +1.3 3,790,957 + htrL b3618 0.13 -0.8 3,790,959 - htrL b3618 0.13 +0.3 3,790,960 + htrL b3618 0.13 -0.7 3,790,960 + htrL b3618 0.13 +0.1 3,790,963 - htrL b3618 0.13 +0.3 3,790,963 - htrL b3618 0.13 +0.3 3,790,963 - htrL b3618 0.13 +0.5 3,790,965 - htrL b3618 0.14 -0.5 3,790,968 - htrL b3618 0.14 -0.3 3,790,968 - htrL b3618 0.14 +1.3 3,790,984 - htrL b3618 0.16 +1.0 3,790,987 + htrL b3618 0.16 -1.2 3,790,992 - htrL b3618 0.17 +0.7 3,790,992 - htrL b3618 0.17 -2.2 3,791,016 - htrL b3618 0.19 +0.1 3,791,016 - htrL b3618 0.19 -0.5 3,791,156 - htrL b3618 0.36 +0.3 3,791,156 - htrL b3618 0.36 -0.3 3,791,164 - htrL b3618 0.37 -0.3 3,791,183 - htrL b3618 0.39 +1.3 3,791,191 - htrL b3618 0.40 +0.3 3,791,191 - htrL b3618 0.40 -0.3 3,791,193 - htrL b3618 0.40 +0.3 3,791,203 - htrL b3618 0.41 -0.3 3,791,246 - htrL b3618 0.46 +1.3 3,791,247 - htrL b3618 0.46 +1.3 3,791,270 - htrL b3618 0.49 +1.3 3,791,271 - htrL b3618 0.49 +2.3 3,791,304 - htrL b3618 0.53 +0.3 3,791,304 - htrL b3618 0.53 +1.3 3,791,304 - htrL b3618 0.53 +1.3 3,791,316 - htrL b3618 0.54 +1.3 3,791,316 - htrL b3618 0.54 +1.3 3,791,347 - htrL b3618 0.58 +1.3 3,791,359 - htrL b3618 0.59 +1.3 3,791,360 - htrL b3618 0.60 +1.3 3,791,361 - htrL b3618 0.60 -0.7 3,791,361 - htrL b3618 0.60 +0.3 3,791,361 - htrL b3618 0.60 +0.3 3,791,361 - htrL b3618 0.60 +0.3 3,791,361 - htrL b3618 0.60 +1.3 3,791,419 - htrL b3618 0.66 +1.3 3,791,571 - htrL b3618 0.84 +1.3 3,791,571 - htrL b3618 0.84 +1.3
Or see this region's nucleotide sequence