Strain Fitness in Escherichia coli BW25113 around b3449

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntggt and yhhA are separated by 119 nucleotidesyhhA and ugpQ overlap by 14 nucleotidesugpQ and ugpC overlap by 4 nucleotides b3447: ggt - gamma-glutamyltranspeptidase periplasmic precursor (NCBI), at 3,583,104 to 3,584,846 ggt b3448: yhhA - hypothetical protein (NCBI), at 3,584,966 to 3,585,406 yhhA b3449: ugpQ - cytoplasmic glycerophosphodiester phosphodiesterase (NCBI), at 3,585,393 to 3,586,136 ugpQ b3450: ugpC - ATP-binding component of sn-glycerol 3-phosphate transport system (VIMSS), at 3,586,133 to 3,587,203 ugpC Position (kb) 3585 3586 3587Strain fitness (log2 ratio) -2 -1 0 1 2at 3584.403 kb on - strand, within ggtat 3584.462 kb on - strand, within ggtat 3584.552 kb on - strand, within ggtat 3584.574 kb on - strand, within ggtat 3584.659 kb on + strand, within ggtat 3584.659 kb on + strand, within ggtat 3584.701 kb on - strandat 3584.701 kb on - strandat 3584.735 kb on + strandat 3584.735 kb on + strandat 3584.735 kb on + strandat 3584.741 kb on - strandat 3584.804 kb on + strandat 3584.861 kb on + strandat 3584.867 kb on + strandat 3584.874 kb on + strandat 3584.894 kb on - strandat 3584.901 kb on + strandat 3584.901 kb on + strandat 3584.993 kb on + strandat 3585.047 kb on - strand, within yhhAat 3585.047 kb on - strand, within yhhAat 3585.069 kb on + strand, within yhhAat 3585.099 kb on - strand, within yhhAat 3585.164 kb on - strand, within yhhAat 3585.166 kb on + strand, within yhhAat 3585.166 kb on + strand, within yhhAat 3585.316 kb on + strand, within yhhAat 3585.366 kb on - strandat 3585.369 kb on - strandat 3585.383 kb on + strandat 3585.431 kb on + strandat 3585.436 kb on + strandat 3585.436 kb on + strandat 3585.436 kb on + strandat 3585.462 kb on - strandat 3585.485 kb on - strand, within ugpQat 3585.485 kb on - strand, within ugpQat 3585.503 kb on + strand, within ugpQat 3585.508 kb on - strand, within ugpQat 3585.508 kb on - strand, within ugpQat 3585.531 kb on - strand, within ugpQat 3585.547 kb on + strand, within ugpQat 3585.673 kb on + strand, within ugpQat 3585.673 kb on + strand, within ugpQat 3585.702 kb on - strand, within ugpQat 3585.702 kb on - strand, within ugpQat 3585.838 kb on - strand, within ugpQat 3585.838 kb on - strand, within ugpQat 3585.859 kb on - strand, within ugpQat 3586.093 kb on - strandat 3586.198 kb on - strandat 3586.198 kb on - strandat 3586.312 kb on - strand, within ugpCat 3586.320 kb on - strand, within ugpCat 3586.396 kb on + strand, within ugpCat 3586.396 kb on + strand, within ugpCat 3586.418 kb on - strand, within ugpCat 3586.418 kb on - strand, within ugpCat 3586.551 kb on + strand, within ugpCat 3586.564 kb on - strand, within ugpCat 3586.564 kb on - strand, within ugpCat 3586.600 kb on - strand, within ugpCat 3586.619 kb on - strand, within ugpCat 3586.619 kb on - strand, within ugpCat 3586.635 kb on - strand, within ugpCat 3586.635 kb on - strand, within ugpCat 3586.675 kb on + strand, within ugpCat 3586.699 kb on + strand, within ugpCat 3586.699 kb on + strand, within ugpCat 3586.718 kb on - strand, within ugpCat 3586.773 kb on + strand, within ugpCat 3586.773 kb on + strand, within ugpCat 3586.834 kb on + strand, within ugpCat 3586.834 kb on + strand, within ugpCat 3586.924 kb on - strand, within ugpCat 3586.929 kb on + strand, within ugpCat 3586.936 kb on + strand, within ugpCat 3586.936 kb on + strand, within ugpCat 3586.952 kb on + strand, within ugpCat 3586.952 kb on + strand, within ugpC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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3,584,403 - ggt b3447 0.75 -0.2
3,584,462 - ggt b3447 0.78 +0.8
3,584,552 - ggt b3447 0.83 +0.9
3,584,574 - ggt b3447 0.84 +0.2
3,584,659 + ggt b3447 0.89 +0.3
3,584,659 + ggt b3447 0.89 +0.6
3,584,701 - +0.1
3,584,701 - +0.3
3,584,735 + +0.8
3,584,735 + +0.2
3,584,735 + -0.1
3,584,741 - +0.5
3,584,804 + -0.3
3,584,861 + +0.7
3,584,867 + +1.1
3,584,874 + +0.2
3,584,894 - +0.2
3,584,901 + +1.0
3,584,901 + +0.1
3,584,993 + -0.1
3,585,047 - yhhA b3448 0.18 -1.0
3,585,047 - yhhA b3448 0.18 -0.1
3,585,069 + yhhA b3448 0.23 -2.3
3,585,099 - yhhA b3448 0.30 +0.2
3,585,164 - yhhA b3448 0.45 +0.4
3,585,166 + yhhA b3448 0.45 +0.2
3,585,166 + yhhA b3448 0.45 -0.0
3,585,316 + yhhA b3448 0.79 +0.4
3,585,366 - -1.0
3,585,369 - -1.3
3,585,383 + -0.0
3,585,431 + +0.0
3,585,436 + +0.2
3,585,436 + +0.8
3,585,436 + +1.5
3,585,462 - -0.6
3,585,485 - ugpQ b3449 0.12 -0.4
3,585,485 - ugpQ b3449 0.12 +0.7
3,585,503 + ugpQ b3449 0.15 -1.2
3,585,508 - ugpQ b3449 0.15 +0.1
3,585,508 - ugpQ b3449 0.15 -0.6
3,585,531 - ugpQ b3449 0.19 -0.6
3,585,547 + ugpQ b3449 0.21 +1.5
3,585,673 + ugpQ b3449 0.38 -0.2
3,585,673 + ugpQ b3449 0.38 +1.1
3,585,702 - ugpQ b3449 0.42 +0.4
3,585,702 - ugpQ b3449 0.42 +0.2
3,585,838 - ugpQ b3449 0.60 +0.6
3,585,838 - ugpQ b3449 0.60 +0.6
3,585,859 - ugpQ b3449 0.63 +0.6
3,586,093 - +0.3
3,586,198 - -1.9
3,586,198 - +0.7
3,586,312 - ugpC b3450 0.17 -0.1
3,586,320 - ugpC b3450 0.17 -0.0
3,586,396 + ugpC b3450 0.25 -0.7
3,586,396 + ugpC b3450 0.25 -0.8
3,586,418 - ugpC b3450 0.27 -1.7
3,586,418 - ugpC b3450 0.27 -2.1
3,586,551 + ugpC b3450 0.39 +0.0
3,586,564 - ugpC b3450 0.40 -0.1
3,586,564 - ugpC b3450 0.40 +0.3
3,586,600 - ugpC b3450 0.44 +0.4
3,586,619 - ugpC b3450 0.45 +1.2
3,586,619 - ugpC b3450 0.45 -1.6
3,586,635 - ugpC b3450 0.47 +0.1
3,586,635 - ugpC b3450 0.47 +0.9
3,586,675 + ugpC b3450 0.51 +2.2
3,586,699 + ugpC b3450 0.53 -2.0
3,586,699 + ugpC b3450 0.53 -1.3
3,586,718 - ugpC b3450 0.55 +0.6
3,586,773 + ugpC b3450 0.60 -1.4
3,586,773 + ugpC b3450 0.60 +0.5
3,586,834 + ugpC b3450 0.65 +1.5
3,586,834 + ugpC b3450 0.65 -0.3
3,586,924 - ugpC b3450 0.74 -0.4
3,586,929 + ugpC b3450 0.74 +0.8
3,586,936 + ugpC b3450 0.75 +0.1
3,586,936 + ugpC b3450 0.75 -1.1
3,586,952 + ugpC b3450 0.76 -0.1
3,586,952 + ugpC b3450 0.76 -0.7

Or see this region's nucleotide sequence